Preparation of glucose sensors using the Langmuir–Blodgett technique

Yasuzawa, Mikito; Hashimoto, Masataka; Fujii, Satoshi; Kunugi, Akira; Nakaya, Tadao

doi:10.1016/s0925-4005(99)00454-2

Cited by 25 publications

(13 citation statements)

References 6 publications

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“…Other applications including biomolecular separation [2][3][4], drug delivery [5], biosensors [6][7][8][9][10], and biomimetic catalysis can also be performed by using supported lipid membranes. Phospholipids, the essential components of biological membranes, are the most widely used materials in preparing lipid films with monolayer or bilayer structures to mimic the biomembrane structures.…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic characteristics and Langmuir–Blodgett deposition behavior of mixed DPPA/DPPC monolayers at air/liquid interfaces

Lee

Lin

Chang

2006

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

Thermodynamic characteristics and Langmuir–Blodgett deposition behavior of mixed DPPA/DPPC monolayers at air/liquid interfaces

Lee

Lin

Chang

2006

Journal of Colloid and Interface Science

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“…Yasuzawa et al also reported that a novel glucose sensor was fabricated using the LB technique with a phospholipid analogous vinyl polymer, 2-[2-methacryloyloxy)-ethyldimethyl-ammonium]ethyl-5¯-cholesten-3-yl phosphate polymer (PCP) [130]. The ordered monolayers of PCP were easily prepared at the interface of air-aqueous solution containing glucose oxidase, and could be transferred onto the platinum wire electrode.…”

Section: Langmuir-blodgett (Lb) Techniquesmentioning

confidence: 99%

Recent progress of phospholipid polymers

Nakaya¹,

Li²

2003

Designed Monomers and Polymers

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Since in 1982 the rst phosphatidylcholineanalogues-containingvinyl monomer 2-(methacryloyloxy)ethyl-2-(trimethylammonium)ethyl phosphate (MTP) was reported, more and more attention has focused on its polymers, as phospholipid polymers have been found to show excellent biocompatibility. We reviewed the progress of phospholipid polymers in 1997 which covered literature from 1974 to 1997; and mainly focused on phosphatidylcholineanalogues-containingvinyl polymers. This review re ects mainly recent research (i.e., mostly later than 1997) and important development progress on polymeric polyphospholipid analogues. A variety of approaches for design, synthesis, characterization, property investigation and potential applications for phospholipid-analoguecontaining polyurethanes and 2-methacryloyloxyethylphosphorylcholine-containing polymers have been extensivelydiscussed. The polymeric phospholipid analogues include polymers such as side-and main-chain types of phosphatidylcholine-analogue-containing polyurethanes, side-and main-chain types of phosphatidylcholine-analogue-containing segmented polyurethanes, phosphatidylcholineanalogue-grafted polyurethanes, 2-methacryloyloxyethylphosphorylcholine-containing polymers, 2-methacryloyloxyethylphosphorylcholine-modi ed acrylamide hydrogel, etc. Design and synthesis of phosphatidylcholine-analogue-containing diols and their polyurethanes with various commercially available diisocyanates and phosphatidylcholine-analogue surface-modi ed polyurethanes are described. New ring-opening reagents such as 2-dimethylaminoethanol for the main-chain type of phosphatidylcholine-analogue-containing polyurethanes, N -methyldiethanolamine for the sidechain type of phosphatidylcholine-analogue-containing polyurethanesand trimethylamine for grafting phosphatidylcholine-analogue-containing vinyl monomers are discussed. A general reaction route to design and synthesize phosphatidylcholine-analogue-containing diols and polyurethanes is summarized. Various phosphatidylcholine-analogue-containing segmented polyurethanes are also discussed. Various phosphatidylcholine-analogue-containing diols, together with 1,4-butanediol or ethylenediamine as chain extender were used to synthesize phosphatidylcholine-analogue-containing segmented polyurethanes or polyurethaneureas, which are based on diphenylmethane diisocyanate and various types of soft segments such as a polycarbonate,polyether, polyester and hydrocarbon diols. Character-310 T. Nakaya and Y. Li ization techniques for phosphatidylcholine-analogue-containing polyurethanes and polymers are also described. These include infra-red spectroscopy, 1 H-NMR, viscosity and gel-permeation chromatography measurements for bulk property; X-ray diffraction methods for structure; dynamic viscoelasticity and tensile measurements for mechanical properties. Moreover, attenuated total re ectanceFourier transform infrared spectroscopy,electron spectroscopy for chemical analysis and contact angle measurements for surface properties are all described. Hemocompatibility ...

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“…Lipids and proteins are the main components of cell membranes. The interaction between protein and lipids is an issue of fundamental interest that has been studied for decades. − These studies are important not only to the understanding of biological systems but also to the development of biomolecular devices such as biosensors. − …”

Section: Introductionmentioning

confidence: 99%

Headgroup Effects of Template Monolayers on the Adsorption Behavior and Conformation of Glucose Oxidase Adsorbed at Air/Liquid Interfaces

et al. 2011

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Stearic acid (SA) and octadecylamine (ODA) monolayers at the air/liquid interface were used as template layers to adsorb glucose oxidase (GOx) from aqueous solution. The effect of the template monolayers on the adsorption behavior of GOx was studied in terms of the variation of surface pressure, the evolution of surface morphology observed by BAM and AFM, and the conformation of adsorbed GOx. The results show that the presence of a template monolayer can enhance the adsorption rate of GOx; furthermore, ODA has a higher ability, compared to SA, to adsorb GOx, which is attributed to the electrostatic attractive interaction between ODA and GOx. For adsorption performed on a bare surface or on an SA monolayer, the surface pressure approaches an equilibrium value (ca. 8 mN/m) after 2 to 3 h of adsorption and remains nearly constant in the following adsorption process. For the adsorption on an ODA monolayer, the surface pressure will increase further 1 to 2 h after approaching the first equilibrium pressure, which is termed the second adsorption stage. The measurement of circular dichroism (CD) spectroscopy indicates that the Langmuir-Blodgett films of adsorbed GOx transferred at the first equilibrium state (π = 8 mN/m) have mainly a β-sheet conformation, which is independent of the type of template monolayers. However, the ODA/GOx LB film transferred at the second adsorption stage has mainly an α-helix conformation. It is concluded that the specific interaction between ODA and GOx not only leads to a higher adsorption rate and adsorbed amount of GOx but also induces a conformation change in adsorbed GOx from β-sheet to α-helix. The present results indicate that is possible to control the conformation of adsorbed protein by selecting the appropriate template monolayer.

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Preparation of glucose sensors using the Langmuir–Blodgett technique

Cited by 25 publications

References 6 publications

Thermodynamic characteristics and Langmuir–Blodgett deposition behavior of mixed DPPA/DPPC monolayers at air/liquid interfaces

Thermodynamic characteristics and Langmuir–Blodgett deposition behavior of mixed DPPA/DPPC monolayers at air/liquid interfaces

Recent progress of phospholipid polymers

Headgroup Effects of Template Monolayers on the Adsorption Behavior and Conformation of Glucose Oxidase Adsorbed at Air/Liquid Interfaces

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