Preparation of Glycopolymer-Modified Gold Nanoparticles and a New Approach for a Lateral Flow Assay

Ishii, Jin; Toyoshima, Masayuki; Chikae, Miyuki; Takamura, Yuzuru; Miura, Yoshiko

doi:10.1246/bcsj.20100303

Cited by 25 publications

(28 citation statements)

References 19 publications

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“…This method has the advantage that the polymer can be fully characterized and preliminary tests can be performed before the immobilization step is conducted. The immobilization is achieved often via reaction of a thiol endgroup with a gold surface . However, for the synthesis of functionalized surfaces for biological applications like affinity chromatography, flat gold surface or gold nanoparticles are not the best choice due to the low surface area and the high costs, respectively.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Thermoresponsive Glycopolymers Combining RAFT Polymerization, Thiol‐Ene Reaction, and Subsequent Immobilization onto Solid Supports

Ehe

Weber

Wagner

et al. 2014

Macro Chemistry & Physics

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A new monomer, N‐hexenylacrylamide, is copolymerized with N‐isopropylacrylamide by reversible addition‐fragmentation chain transfer (RAFT) polymerization using a carboxylic‐acid‐functionalized RAFT agent. Subsequently, the resulting functional copolymers are reacted with acetylated thiogalactose via a thiol‐ene reaction. After deprotection of the sugar acetyl groups, the glycopolymers show a lower critical solution temperature (LCST) behavior, as confirmed by turbidity measurements. One of these glycopolymers is subsequently immobilized onto solid supports. The obtained products are characterized by attenuated total reflection Fourier transform IR (ATR FTIR) spectroscopy, thermal gravimmetric analysis (TGA), and elemental analysis, confirming the immobilization of the glycopolymer. Additionally, the glycopolymer‐functionalized support shows interaction with the lectin RCA120.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Thermoresponsive Glycopolymers Combining RAFT Polymerization, Thiol‐Ene Reaction, and Subsequent Immobilization onto Solid Supports

Ehe

Weber

Wagner

et al. 2014

Macro Chemistry & Physics

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“…The obtained gold nanoparticles and substrates are used as biosensors in lateral-flow assays and electrochemistry. 123,124 The glycopolymer-modified substances showed large bioaffinities as a result of their multivalency, and they exhibited high specificities because the glycopolymer is bioinert, except toward lectin because of its high hydrophilicity.…”

Section: Synthesis Of Functional Glyco-materials Via Living Polymerizmentioning

confidence: 99%

Design and synthesis of well-defined glycopolymers for the control of biological functionalities

Miura

2012

Polym J

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131

104

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This review addresses the design and synthesis of synthetic glycopolymers. Glycopolymers with pendant saccharides exhibit high affinities for proteins owing to their multivalency. Glycopolymers have molecular recognition abilities and amphiphilicity and can be applied as biomaterials and in bioassays. Most synthetic glycopolymers are prepared from polymerizable saccharide derivatives, and the rest are prepared by saccharide addition to a polymer backbone. Because sugar-recognizing proteins have precise structures, the living polymerization of glycopolymers is important in the fabrication of well-defined multiple ligands of saccharides. Glycopolymers with narrow polydispersity are useful for determining the interactions between saccharides and proteins. The living polymerization of glycopolymers provides hybrid materials by the terminal modification of the polymers. The glycopolymers of block polymers, star polymers and polymer brushes have been investigated for use in novel biomaterials.

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“…GNPs functionalized with poly(M76-stat-AM) were also employed in a lateral flow assay for the detection of proteins: A test solution of ConA at 0.01 μg mL −1 was readily detectable with the glycopolymer-modified GNPs with a sugar ratio of 6% (Entry 184, Table 3) [176]. Finally, the same type of GNPs were used in an electrochemical assay for the detection of ConA [222].…”

Section: Scheme 23mentioning

confidence: 99%

Synthesis of Glycopolymer Architectures by Reversible-Deactivation Radical Polymerization

Ghadban

Albertin

2013

Polymers

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This review summarizes the state of the art in the synthesis of well-defined glycopolymers by Reversible-Deactivation Radical Polymerization (RDRP) from its inception in 1998 until August 2012. Glycopolymers architectures have been successfully synthesized with four major RDRP techniques: Nitroxide-mediated radical polymerization (NMP), cyanoxyl-mediated radical polymerization (CMRP), atom transfer radical polymerization (ATRP) and reversible addition-fragmentation chain transfer (RAFT) polymerization. Over 140 publications were analyzed and their results summarized according to the technique used and the type of monomer(s) and carbohydrates involved. Particular emphasis was placed on the experimental conditions used, the structure obtained (comonomer distribution, topology), the degree of control achieved and the (potential) applications sought. A list of representative examples for each polymerization process can be found in tables placed at the beginning of each section covering a particular RDRP technique.

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Preparation of Glycopolymer-Modified Gold Nanoparticles and a New Approach for a Lateral Flow Assay

Cited by 25 publications

References 19 publications

Synthesis of Thermoresponsive Glycopolymers Combining RAFT Polymerization, Thiol‐Ene Reaction, and Subsequent Immobilization onto Solid Supports

Synthesis of Thermoresponsive Glycopolymers Combining RAFT Polymerization, Thiol‐Ene Reaction, and Subsequent Immobilization onto Solid Supports

Design and synthesis of well-defined glycopolymers for the control of biological functionalities

Synthesis of Glycopolymer Architectures by Reversible-Deactivation Radical Polymerization

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