Preparation of High-quality Glabridin Extract from Glycyrrhiza glabra

Hong, Jongki; Jung, In Il; Cho, Yun Kyoung; Haam, Seungjoo; Lee, Sang-Yun; Lim, Gio‐Bin; Ryu, Jong Hoon

doi:10.1007/s12257-019-0121-7

Cited by 9 publications

(3 citation statements)

References 35 publications

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“…Lv et al describe the extraction of glabridin using EA with a yield of 0.23% (Lv, 2010). Our present reporting ~0.29% yield of glabridin from licorice with EA is highest to date with the time of extraction to a maximum of 6 h as against 24 h and 48 h extraction times reported earlier (Tian, 2008;Hong, 2019). Since the solvent volumes used in this study is close to the volumes of solvents used by other workers, the higher yield of glabridin with EA seen is most probably due to the property of the EA solvent used for extraction.…”

Section: Discussionsupporting

confidence: 63%

“…In the context of glabridin extraction from licorice, solvents that have been used to date include water, methanol, ethanol, acetonitrile and chloroform (Tian, 2008). Acetone (Ao, 2015), EA (Xu, 2009;Lv, 2010), supercritical carbon dioxide (SC-CO 2 ) with ethanol (Hong, 2019) and 95% ethanol (Nakagawa, 2002) are some of the solvents used for glabridin extraction.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Solvent Polarity on Extraction Yield of Total Flavonoids with Special Emphasis to Glabridin from Glycyrrhiza glabra Roots

Yewale¹,

FARASH²,

Kulkarni³

et al. 2022

Fabad Journal of Pharmaceutical Sciences

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Different organic solvents (ethanol, dichloromethane, ethyl acetate and acetone) were studied for their effects on the extraction efficiency of glabridin and total flavonoids (TF) from Glycyrrhiza glabra roots. The extract yield of Glycyrrhiza glabra roots was in the range of 3% to 6% following the extraction efficiency in the order ethanol>acetone>ethyl acetate>dichloromethane. A higher extraction yield of TF and glabridin was obtained with dichloromethane, followed by ethyl acetate, acetone and ethanol, indicating that the non-polar solvents help in optimal extraction of TF and glabridin. We also demonstrate for the first time, that the extraction efficiency of the flavonoids is not significantly affected by the use of the recovered solvents except in case of ethanol which reflects that the moistureabsorbing capacity of the solvent dictates the extraction efficiency of such compounds. The glycyrrhizin content in all the extract types was rather low (0.1 % to 1%) except for extract prepared with water, where the glycyrrhizin content was ~10% as expected since glycyrrhizin is a polar compound. Interestingly, we observed that ethyl acetate selectively isolated only glabridin with no traces of glycyrrhizin, which is a finding reported for the first time.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Effect of Solvent Polarity on Extraction Yield of Total Flavonoids with Special Emphasis to Glabridin from Glycyrrhiza glabra Roots

Yewale¹,

FARASH²,

Kulkarni³

et al. 2022

Fabad Journal of Pharmaceutical Sciences

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“…Glabridin (GBN) is a flavonoid and one of the main active ingredients of Glycyrrhiza glabra L [ 10 ]. At present, GBN has a variety of pharmacological activities, including antiatherosclerosis [ 11 ], blood lipid reduction [ 12 ], nervous system protection [ 13 ], and anti-inflammatory [ 14 ] actions.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Effects of Glabridin on the Proliferation, Apoptosis, and Migration of the Human Colon Cancer Cell Lines SW480 and SW620 and Its Mechanism Based on Reverse Virtual Screening and Proteomics

Xia

et al. 2023

Oxidative Medicine and Cellular Longevity

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Colon cancer is a relatively common malignant tumor of the digestive tract. Currently, most colon cancers originate from adenoma carcinogenesis. By screening various licorice flavonoids with anticancer effects, we found that glabridin (GBN) has a prominent anticolon cancer effect. First, we initially explored whether GBN can inhibit proliferation, migration, and invasion and induce apoptosis in SW480 and SW620 cells. Next, we exploited reverse virtual and proteomics technologies to screen out closely related target pathways on the basis of a drug and target database. At the same time, we constructed the structure of the GBN target pathway in colon cancer. We predicted that GBN can regulate the phosphatidylinositol 3-kinase (PI3K)–protein kinase B (AKT)–mammalian target of the rapamycin pathway (mTOR) pathway to fight colon cancer. Finally, through Western blot analysis and qRT-PCR, we verified that the expression levels of the PI3K, AKT, and mTOR proteins and genes in this pathway were significantly reduced after GBN administration. In short, the promising discovery of the anticolon cancer mechanism of GBN provides a reliable experimental basis for subsequent new drug development.

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Quaternary solvent system for highly efficient separation and purification of glabridin by fractional extraction

Wang

Yang

Tang

2022

Chemical Engineering Science

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Preparation of High-quality Glabridin Extract from Glycyrrhiza glabra

Cited by 9 publications

References 35 publications

Effect of Solvent Polarity on Extraction Yield of Total Flavonoids with Special Emphasis to Glabridin from Glycyrrhiza glabra Roots

Effect of Solvent Polarity on Extraction Yield of Total Flavonoids with Special Emphasis to Glabridin from Glycyrrhiza glabra Roots

Investigation of the Effects of Glabridin on the Proliferation, Apoptosis, and Migration of the Human Colon Cancer Cell Lines SW480 and SW620 and Its Mechanism Based on Reverse Virtual Screening and Proteomics

Quaternary solvent system for highly efficient separation and purification of glabridin by fractional extraction

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