Preparation of human milk fat substitutes similar to human milk fat by enzymatic acidolysis and physical blending

Wang, Xiaosan; Huang, Zhuoneng; Lee, Hua; Feng, Zhiyong; Cheng, Xinyi

doi:10.1016/j.lwt.2020.110818

Cited by 15 publications

(7 citation statements)

References 30 publications

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“…Yuan et al (2020) only focused on the addition of MCFAs and the content of PA at the sn-2 position, and the content of sn-2 PA in the final product was only 30%. Wang et al (2021) prepared a product with high sn-2 PA content (87%), but without MCFAs and essential fatty acids. Compared with these products, HMFS prepared in this study contained both FAs sn-2 PA.…”

Section: Fatty Acid Profilementioning

confidence: 99%

“…The unique TAG structure of HMF is associated with digestion, absorption, and metabolism of fats and nutrients in infants. It was reported that more than 60% sn-2 fatty acids (mainly PA) were remained after digestion, which could improve the absorption and utilization of essential fatty acids and calcium to prevent infant constipation (Wang et al, 2021). However, for a variety of reasons, many infants cannot be breastfed, in which case the infants require other sources of energy and nutrition, such as infant formula.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Zou et al (2016a) prepared 1,3-dioleoyl-2-palmitoylglycerol (OPO)-rich HMFS by Lipozyme RM IM-catalyzed acidolysis of basa catfish oil (solid fraction) with oleic acid containing high content of sn-2 PA (57.80%). Wang et al (2021) developed a physical blending model to prepare HMFS rich in 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL), and the contents of OPL and sn-2 PA in the synthesized OPL product were 47.93% and 87.90%, respectively. However, to our knowledge, these studies mainly focused on the major TAG structures (OPO and OPL) and sn-2 PA content in HMFS.…”

Section: Introductionmentioning

confidence: 99%

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Construction and in vitro digestibility evaluation of a novel human milk fat substitute rich in structured triglycerides

et al. 2022

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The infants who cannot be breastfeed need to obtain energy and nutrition from other sources, such as formula. In this study, a human milk fat substitute (HMFS) rich in structured triglycerides (STG) and with a variety of fatty acids was prepared by one-step transesterification reaction. The optimum conditions for the lipase-catalyzed transesterification between basa catfish oil solid fraction, Cinnamomum camphora seed kernel oil, linseed oil, microbial oil and algae oil were studied and the in vitro digestibility of the constructed HMFS was investigated. Under the optimal conditions, the obtained HMFS contained 58.86% STG and 39.73% sn-2 palmitic acid (PA). Fatty acid composition analysis showed that HMFS was composed of capric acid (Ca), lauric acid (La), oleic acid (O), linoleic acid (L), linolenic acid (Ln), arachidonic acid (ARA) and docosahexaenoic acid (DHA). LC-MS/MS analysis found that 142 new triglycerides were generated, such as Ca-P-ARA, Ca-P-Ln, La-P-ARA, La-P-L and P-O-ARA. Moreover, compared with physical mixture (PM), HMFS had better melting and crystallization temperature and the fatty acid release rate of HMFS was significantly improved. These results suggested that HMFS may have a great application in infant food as a nutritional ingredient.

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Section: Fatty Acid Profilementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Construction and in vitro digestibility evaluation of a novel human milk fat substitute rich in structured triglycerides

et al. 2022

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“…The first two classic scoring models have been applied for evaluations by researchers [200][201][202][203][204]. Zou et al [205] evaluated the potential of mammalian milk fats for HMFSs based on the total and sn-2 fatty acid, TAG, phospholipid and phospholipid fatty acid compositions.…”

Section: Evaluation Of Hmfssmentioning

confidence: 99%

Preparation of Human Milk Fat Substitutes: A Review

Jiang

Zou

Chao

et al. 2022

Life

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Human milk is generally regarded as the best choice for infant feeding. Human milk fat (HMF) is one of the most complex natural lipids, with a unique fatty acid composition and distribution and complex lipid composition. Lipid intake in infants not only affects their energy intake but also affects their metabolic mode and overall development. Infant formula is the best substitute for human milk when breastfeeding is not possible. As the main energy source in infant formula, human milk fat substitutes (HMFSs) should have a composition similar to that of HMF in order to meet the nutritional needs of infant growth and development. At present, HMFS preparation mainly focuses on the simulation of fatty acid composition, the application of structured lipids and the addition of milk fat globule membrane (MFGM) supplements. This paper first reviews the composition and structure of HMF, and then the preparation development of structured lipids and MFGM supplements are summarized. Additionally, the evaluation and regulation of HMFSs in infant formula are also presented.

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“…The significance of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol has been recognized due to its high content in human milk fat. In specific regions, the content of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol in human milk fat is even higher than that of 1,3-dioleoyl-2-palmitoylglycerol as a result of different dietary habits, such as in China. , However, compared to the synthesis of 1,3-dioleoyl-2-palmitoylglycerol, 1-oleoyl-2-palmitoyl-3-linoleoylglycerol has relatively complex fatty acid composition, resulting in less research and greater difficulty in synthesis . By systematically studying the synthesis, stability, and performance of SOM–CuBTC, as well as the effects of hydrophobic modification on lipase immobilization, this study aims to provide valuable insights into the design and development of efficient and stable MOF-based carriers for lipase-catalyzed reactions.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Stability and Performance of Immobilized Lipase Using Hydrophobically Modified Single-Crystalline Ordered Macro–Microporous CuBTC as a Carrier Material

Liu,

Dai,

Liu

et al. 2024

ACS Sustainable Chem. Eng.

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Single-crystalline ordered macro−microporous CuBTC (SOM−CuBTC) is a promising carrier for lipase immobilization due to enhanced mass transfer and stability toward fatty acids. However, the low yield per mass of the template during the preparation process and the water instability of the SOM−CuBTC carrier have posed significant limitations on its practical applications. In this study, we addressed these challenges by introducing a novel dual-solvent system consisting of dimethyl sulfoxide (DMSO) and ethanol to obtain a stable precursor solution with a concentration approximately 10 times higher than that in previous literature, yielding 21.4 mg of SOM−CuBTC per gram of the polystyrene template. However, the decomposition of SOM− CuBTC in an aqueous system of lipase immobilization was observed. We explored chemical vapor deposition and sol−gel methods for hydrophobic modification on SOM−CuBTC. SOM−CuBTC coated by hydrophobic polydimethylsiloxane (PDMS) via the sol− gel method possessed excellent chemical stability and exhibited great potential for lipase immobilization with a significant increase by 98.7% in the specific activity. The obtained immobilized lipase not only showed improved thermal stability and pH tolerance but also displayed excellent catalytic performance in the synthesis process of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) by acidolysis. This work reveals the great potential of SOM−CuBTC and provides new insights into the rational design of metal− organic frameworks for enzyme immobilization in extensive applications.

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Preparation of human milk fat substitutes similar to human milk fat by enzymatic acidolysis and physical blending

Cited by 15 publications

References 30 publications

Construction and in vitro digestibility evaluation of a novel human milk fat substitute rich in structured triglycerides

Construction and in vitro digestibility evaluation of a novel human milk fat substitute rich in structured triglycerides

Preparation of Human Milk Fat Substitutes: A Review

Enhanced Stability and Performance of Immobilized Lipase Using Hydrophobically Modified Single-Crystalline Ordered Macro–Microporous CuBTC as a Carrier Material

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