Preparation of human serum albumin nanoparticles using a chemometric technique

Abstract

Human serum albumin (HSA), a versatile protein carrier for drug delivery, is an ideal material to fabricate nanoparticles for drug delivery systems. These nanoparticles can accumulate in tumor interstitium due to the enhanced permeability and retention effect. The most important characteristics of HSA nanoparticles are particle size, shape, and zeta potential. A chemometric approach was applied for HSA nanoparticles' size optimization in this study. The effects of three experimental parameters; pressure (P) or power, organic solvent volume (V), and time (T), were investigated under sonication and high-pressure homogenization, using multivariate analysis. The trials were performed based on the Box-Behnken experimental design. The criteria for the appraisal of the descriptive ability of a multinomial were R 2 = 0.819, standard error = 20.420, and F-ratio = 19.550. The method was optimized with respect to the nanoparticles' size as a response. The Box-Behnken experimental design was applied to optimize and trial the robustness of the HSA nanoparticle preparation method.

Design and evaluation of albumin nanoparticles for the delivery of a novel β-tubulin polymerization inhibitor

“…This method takes advantage of the tendency of hydrophobic drugs in an o/w emulsion to interact with albumin in water upon evaporation of the organic solvent (53). In previous studies, Hosseinifar et al (54) have investigated the use of both sonicator as well as high-pressure homogenization on the physicochemical properties of HSA nanoparticles. In agreement with their results, we have found the use of high-pressure homogenization to be more reliable and effective than sonication in achieving BSA nanoparticles of smaller diameters (Figure 4).…”

Preparation and Characterization of Albumin Nanoparticles of Paclitaxel-Triphenylphosphonium Conjugates: New Approach to Subcellular Targeting

“…The mixture was lyophilized (Christ, ALPHA 2-4 LD plus Osterode am Harz, Germany) to give a white powder and it was kept at 2-8 ° C for further experiments. Moreover, the preparation of HSA nanoparticles using a chemometric technique has been previously explained [21].…”

Doxorubicin-loaded, pH-sensitive Albumin Nanoparticles for Lung Cancer Cell Targeting

“…17,18 Nanotechnology utilizes various chemistries to fabricate such nanostructures, including a variety of natural materials, 19 one of which is serum albumin protein. 20 Albumin protein is nontoxic and non-immunogenic and also has a high stability and high solubility in water, which has attracted the attention of researchers in this field. 21,22 So far, different types of this protein have been extracted for various biomedical applications.…”