Preparation of hydrophilic molecularly imprinted polymers via bulk polymerization combined with hydrolysis of ester groups for selective recognition of iridoid glycosides

Abstract

Hydrophilic molecularly imprinted polymers (H-MIP) with molecular recognition ability for iridoid glycosides (IGs) have been obtained via bulk polymerization combined with hydrolysis of ester groups. H-MIP were characterized by Fourier transform infrared spectroscopy (FT-IR). The hydrophilcity was measured by the contact angle measurement and the water dispersion stability. The obtained H-MIP demonstrated high selectivity and specific binding ability to five IGs in aqueous media. The group extraction efficiency of molecular imprinted solid-phase extraction (MISPE) for five IGs was investigated, including loading sample, breakthrough volume, washing solvent, and elution solvent. Compared with non-imprinted solid-phase extraction (NISPE), the higher average recovery (95.5 %) of five IGs with lower relative standard deviations values (below 6.1 %) using MISPE combined with high-performance liquid chromatography (HPLC) were achieved at three spiked levels in three blank samples. Under the optimum MISPE conditions, the wide linear range with the correlation coefficient of R (2) ≥ 0.9950 for five IGs with low limits of detection (LOD) and quantification (LOQ) (0.01-0.08 and 0.03-0.27 μg mL(-1), respectively) were obtained. Chromatograms obtained using MISPE columns demonstrated that the matrix interference has been minimized and great interferences around IGs were also eliminated efficiently. These results indicated that the developed MISPE-HPLC method was selective, accurate, and applicable for the determination of IGs in water media. Graphical Abstract Preparation of hydrophilic molecularly imprinted polymers via bulk polymerization combined with hydrolysis of ester groups.

Preparation of five high‐purity iridoid glycosides from Gardenia jasminoides Eills by molecularly imprinted solid‐phase extraction integrated with preparative liquid chromatography

“…Two MISPE methods for the determination of IGs have been developed previously . The same SPE protocol was used here to evaluate the absorbance, and the results are listed in Table .…”

“…It was demonstrated that 10 mg of MIPs could selectively bind 0.73 mg of GDS, compared with 0.18 mg loading for NIPs. The a The values were calculated using the reported method [26]. b The amount of IGs was obtained using the MISPE-prep-HPLC method in this work.…”

“…This approach has been widely used as an alternative cleanup step in place of traditional procedures involving SPE and LLE, filtration, and centrifugation [20]. The potential of MISPE in the selective extraction of active substances from medicinal plants has been demonstrated previously [21][22][23][24][25][26][27][28], but the preparation of highpurity IGs using MIPs has not been reported. This is perhaps in part because of the difficulty of obtaining template molecules and need for the MIPs to recognize a family of IGs, rather than one single compound.…”

Preparation of magnetic molecularly imprinted polymers for the rapid detection of diethylstilbestrol in milk samples

“…[23][24][25][26][27][28] Magnetic molecularly imprinted polymers (MMIPs) that combine molecular imprinting techniques with magnetic separation techniques not only have the imprinting selectivity of the target, but can also be quickly separated under the action of an external magnetic field. [29][30][31][32][33] After target compound adsorption, the magnetic sorbent can be easily isolated from matrix solutions by applying an external magnetic field, without the need for centrifugation or filtration. [34][35][36][37] A MMIP was synthesized by suspension polymerization for selective recognition of dibenzothiophene in gasoline samples.…”

Molecularly imprinted covalent organic polymers for the selective extraction of benzoxazole fluorescent whitening agents from food samples

“…However, COPs tend to lack specific molecular bonding ability. To address this issue, molecularly imprinted polymers (MIPs) have been widely studied, mainly due to its ability of selective recognition for the target molecule and their structural analogues . Novel molecularly imprinted covalent organic polymers (MICOPs) that combine the outstanding properties of both COPs and MIPs are therefore expected to be the popular adsorption media for trace analysis of FWAs in food.…”