Highly selective lead ion imprinted mesoporous silica adsorbents (Pb II -IMS) were prepared through a co-condensation pathway with 3-(g-aminoethylamino)propyltrimethoxysilane (AAPTS) (Pb II -IMS-NN) and 3-aminepropyltriethoxysilyl (APTES) (Pb II -IMS-N) as monomers. The prepared adsorbents were characterised by FT-IR spectroscopy, X-ray photoelectron spectroscopy, power X-ray diffraction, transmission electron microscopy, scanning electron microscopy, thermogravimetric analysis, and nitrogen adsorption-desorption techniques. The results showed that the synthesised adsorbents presented a highly ordered mesoporous structure. In comparison with Pb II -IMS-N, Pb II -IMS-NN demonstrated a higher adsorption capacity in a series of static and dynamic adsorption experiments, and was further applied to a continuous fixed-bed column study under different conditions. It was found that the breakthrough time of the fixed-bed increased with an increase in bed depth, but decreased with increased flow rate and initial Pb II concentration, and the dynamic adsorption data was more consistent with the Thomas model than the Adams-Bohart model. Furthermore, the Pb II -IMS-NN showed a greater recognition and binding affinity towards the target lead ions than Pb II -IMS-N.