Preparation of liposomes by a simple emulsification technique

Laloy, Edith; Vuillemard, Jean-Christophe; Ackermann, Hans-W.; Robin, Olivier

doi:10.1007/bf00151475

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…Some of the major challenges for the delivery of proteins using liposomes are that the processing methods, high membrane permeability, and low liposome stability in vivo present obstacles toward exploitation of therapeutic proteins in the clinic. The relative high cost of the lipid amphiphiles combined with complicated formulation, processing, storage stability, and rehydration have delayed widespread commercialization using this approach, although several liposome formulations for small molecule therapeutics have been approved for use (Myocet, L-AMB, for example).…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for the Encapsulation of Biomolecules into Polymersomes via Direct Hydration

O’Neil

Suzuki

Demurtas³

et al. 2009

Langmuir

103

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One of the major engineering challenges for the implementation of block copolymer vesicles, or polymersomes, as therapeutic drug carriers is obtaining high encapsulation efficiencies for biomolecules. Here we present a novel method for encapsulation of proteins with high encapsulation efficiency within polymersomes formed from block copolymers of poly(ethylene glycol)-bl-poly(propylene sulfide). By formulation of the neat block copolymer with a low molecular weight poly(ethylene glycol), direct hydration of the formulated mixture yielded polymersomes. We were able to achieve encapsulation efficiencies for ovalbumin at 37%, bovine serum albumin at 19%, and bovine gamma-globulin at 15% when the proteins were included in the hydration solution. The formulation process and the dispersion of polymersomes from the preparation in phosphate-buffered saline were characterized using confocal microscopy, cryogenic transmission electron microscopy, and fluorimetry. We were also successful in the encapsulation of proteinase K, a proteolytic enzyme, and demonstrated by SDS-PAGE that the enzyme was contained inside polymersomes when dispersed in a solution of ovalbumin.

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Section: Introductionmentioning

confidence: 99%

A Novel Method for the Encapsulation of Biomolecules into Polymersomes via Direct Hydration

O’Neil

Suzuki

Demurtas³

et al. 2009

Langmuir

103

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“…This mechanochemical method is readily available with a commercial ultra high-pressure homogenizer and needs no organic solvents. However, there have been limited studies on the large-scale preparation of liposomes from edible lipids of low purity by using the method [23][24][25] from the viewpoint of developing the formulations into an efficient carrier of functional food materials.…”

mentioning

confidence: 99%

Efficient Preparation of Liposomes Encapsulating Food Materials Using Lecithins by a Mechanochemical Method

Takahashi

Inafuku²,

Miyagi³

et al. 2007

J. Oleo Sci.

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“…Once the solvent is evaporated, it results in liposome formation. This method ensures higher encapsulation efficiency compared to the injection method [36].…”

Section: Emulsification Methodsmentioning

confidence: 99%

Lipid Nanoparticles: An Effective Tool to Improve the Bioavailability of Nutraceuticals

Ashfaq,

Rasul,

Asghar

et al. 2023

IJMS

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Nano-range bioactive colloidal carrier systems are envisaged to overcome the challenges associated with treatments of numerous diseases. Lipid nanoparticles (LNPs), one of the extensively investigated drug delivery systems, not only improve pharmacokinetic parameters, transportation, and chemical stability of encapsulated compounds but also provide efficient targeting and reduce the risk of toxicity. Over the last decades, nature-derived polyphenols, vitamins, antioxidants, dietary supplements, and herbs have received more attention due to their remarkable biological and pharmacological health and medical benefits. However, their poor aqueous solubility, compromised stability, insufficient absorption, and accelerated elimination impede research in the nutraceutical sector. Owing to the possibilities offered by various LNPs, their ability to accommodate both hydrophilic and hydrophobic molecules and the availability of various preparation methods suitable for sensitive molecules, loading natural fragile molecules into LNPs offers a promising solution. The primary objective of this work is to explore the synergy between nature and nanotechnology, encompassing a wide range of research aimed at encapsulating natural therapeutic molecules within LNPs.

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Preparation of liposomes by a simple emulsification technique

Cited by 7 publications

References 9 publications

A Novel Method for the Encapsulation of Biomolecules into Polymersomes via Direct Hydration

A Novel Method for the Encapsulation of Biomolecules into Polymersomes via Direct Hydration

Efficient Preparation of Liposomes Encapsulating Food Materials Using Lecithins by a Mechanochemical Method

Lipid Nanoparticles: An Effective Tool to Improve the Bioavailability of Nutraceuticals

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