Preparation of magnetic metal organic framework nanocomposites for efficient and selective adsorption of hemoglobin from bovine blood

Yao, Xin; Ma, Xiangdong; Gao, Xiang; Li, Jia

doi:10.1039/c7ra04397j

Cited by 14 publications

(5 citation statements)

References 37 publications

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“…Currently, various strategies have been developed for purifying hemoglobin from biological samples, including molecular imprinting technology (MIT), − immobilized metal affinity chromatography (IMAC), − metal oxide affinity chromatography (MOAC), liquid–liquid extraction (LLE), and so on. Among the existing isolation methods, immobilized metal affinity chromatography (IMAC) is the most powerful technique for the separation and enrichment of target proteins or peptides based on the coordination between the immobilized metal ions and specific amino acids of proteins.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Yb³⁺-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum

Wang

Guan

Han

et al. 2019

ACS Biomater. Sci. Eng.

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In this work, Yb 3+ -immobilized hydrophilic phytic-acid-coated magnetic nanocomposites were prepared through a facile route and used to selectively separatrf bovine hemoglobin. Hydrophilic phytic acid (PA) was coated onto the magnetic Fe 3 O 4 -PEI via electrostatic interactions, followed by finally chelating with Yb 3+ ions, which could produce specific protein binding sites at room temperature in water, and complex instrumentation was not necessary. The performance of as-prepared hybrids (Fe 3 O 4 -PEI-PA-Yb 3+ ) was assessed by selectively isolating bovine hemoglobin (BHb). The obtained maximum binding capacity was 347.3 mg g −1 . The retained BHb could be eluted under simple elution via using 0.1 M of Na 2 CO 3 , giving a recovery of 83%. Moreover, the generation of nanocomposites was demonstrated. In addition, the PA and PEI could improve the hydrophilicity of nanoparticles and further reduce the nonspecific adsorption. Therefore, such nanocomposites were successfully employed to selectively bind and separate BHb from bovine serum as verified by SDS-PAGE and MALDI-TOF MS analysis, providing a new perspective for the isolation of heme proteins in proteomics.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Yb³⁺-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum

Wang

Guan

Han

et al. 2019

ACS Biomater. Sci. Eng.

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“…Magnetic materials play a significant role as the adsorbents of His-proteins because of their magnetism. − Magnetic adsorbents could be easily collected from the solution with a magnet after protein adsorption, which is conducive to its recovery and reuse. − Numerous researchers have designed a variety of magnetic materials to purify His-tagged proteins. − Among these magnetic materials, yolk–shell Fe 3 O 4 @NiSiO 3 performs well because of its large specific surface area. , However, it has been found that it is inefficient in purifying His-proteins with only Fe 3 O 4 @NiSiO 3 , which might be due to the low surface content of nickel ions . Niu and co-workers have loaded Ni NPs on NiSiO 3 by an in situ thermal decomposition and reduction method, but this method is not suitable for Fe 3 O 4 @NiSiO 3 , because Fe 3 O 4 would be reduced and lose its magnetism .…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Yolk–Shell Fe₃O₄@NiSiO₃/Ni Microspheres for Efficient Purification of Histidine-Rich Proteins

Wang

Wei

et al. 2021

Langmuir

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Magnetic materials perform well in the purification of histidine-rich proteins (His-proteins). In this work, a facile fabrication of yolk−shell magnetic Fe 3 O 4 @NiSiO 3 /Ni microspheres for the efficient purification of His-proteins has been reported. Yolk−shell Fe 3 O 4 @NiSiO 3 microspheres were prepared via hydrothermal reaction. Then Ni nanoparticles (NPs) were loaded on Fe 3 O 4 @NiSiO 3 microspheres after the adsorption and in situ reduction of nickel acetylacetonate. The yolk−shell Fe 3 O 4 @ NiSiO 3 /Ni microspheres had a hierarchical flower-like structure and large cavities. The size of the cavity depended on the reaction time. This indicated that the microspheres had a large specific surface area for loading of more Ni NPs, which was crucial to the high His-protein adsorption capacity of Fe 3 O 4 @NiSiO 3 /Ni microspheres. Fe 3 O 4 @NiSiO 3 /Ni microspheres had a high adsorption capacity for bovine hemoglobin (BHb, 2822 mg/g), which was better than the values of other His-protein adsorbents. Fe 3 O 4 @ NiSiO 3 /Ni microspheres still had a high BHb separation efficiency after seven separation cycles, indicating its good reusability and stability. Therefore, the as-prepared bifunctional yolk−shell Fe 3 O 4 @NiSiO 3 /Ni microspheres exhibited great practical application value for His-protein purification.

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“…Para avaliar a adsorção de materiais, foi de suma importância escolher um modelo que mais se aproxima das condições reais da adsorção. Note-se que diferentes modelos de adsorção podem ser adotados para nanomateriais 10,14,15,28,31,36,37,41,51,63,[65][66][67]69 As análises de capacidade máxima de adsorção para os sais de cobalto mostraram um comportamento semelhante seja para a solução de sulfato de cobalto ou de nitrato de cobalto, destacando-se a solução de nitrato de cobalto que apresenta capacidade de adsorção ligeiramente maior.…”

Section: Metodologiaunclassified

“…O ponto de inflexão ilustra a concentração para a qual a adsorção supera a complexação.Outro modelo muito comum é baseado em hipóteses de reação proposto por Langmuir64 , em 1918. Várias pesquisas vem sendo desenvolvidas utilizando tal modelo10,14,15,17,28,31,36,37,41,51,63,[65][66][67][68][69][70][71][72] .Supõe-se que o sólido tenha uma capacidade de adsorção limitada Qmax. quantidade de sítios livres no sólido.…”

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Estudo da extração hidrometalúrgica de matais de transição com nanopartículas superparamagnéticas funcionalizadas

Kaid¹

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Hydrometallurgical processing of strategic elements such as copper and cobalt is a subject of great relevance because of the economical and environmental issues involved. In this thesis, the interaction of superparamagnetic nanoparticles functionalized with ethylenediamine groups with transition metal ions, such as copper(II) and cobalt(II), has been investigated from the physico-chemical point of view, focusing on the adsorption kinetics and complexation equilibrium involved, aiming the understanding of magnetic nanohydrometallurgy applied to those systems. Also investigated was the influence of possible variables, such as the counter ions in solution and the adsorption selectivity observed in the comparison of different metal ions. Special emphasis was applied to the construction of adsorption isotherms using analytical methods (EDX) and zeta potentials measured in situ in aqueous solution. The proposed models in both cases, provided a critical view of the methodologies, showing the contrasts and the special role of the solvodynamic effects associated with the zeta potentials, including the formation of ion pairs at the nanoparticles slipping plane. The possible application of the systems in the development of magnetic affinity chromatography has also been demonstrated in the selective adsorption of mioglobin by the superparamagnetic nanoparticles containing copper ions. The Langmuir isotherm revealed a reversible process, in which the adsorption and desorption steps can be controlled by the pH of the reactional media.

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Preparation of magnetic metal organic framework nanocomposites for efficient and selective adsorption of hemoglobin from bovine blood

Abstract: Fe3O4@[Cu3(btc)2] particles were prepared and used for efficient and selective removal of abundant BHb from bovine blood.

Cited by 14 publications

References 37 publications

Fabrication of Yb³⁺-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum

Fabrication of Yb³⁺-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum

Fabrication of Yolk–Shell Fe₃O₄@NiSiO₃/Ni Microspheres for Efficient Purification of Histidine-Rich Proteins

Estudo da extração hidrometalúrgica de matais de transição com nanopartículas superparamagnéticas funcionalizadas

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Preparation of magnetic metal organic framework nanocomposites for efficient and selective adsorption of hemoglobin from bovine blood

Abstract: Fe3O4@[Cu3(btc)2] particles were prepared and used for efficient and selective removal of abundant BHb from bovine blood.

Cited by 14 publications

References 37 publications

Fabrication of Yb3+-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum

Fabrication of Yb3+-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum

Fabrication of Yolk–Shell Fe3O4@NiSiO3/Ni Microspheres for Efficient Purification of Histidine-Rich Proteins

Estudo da extração hidrometalúrgica de matais de transição com nanopartículas superparamagnéticas funcionalizadas

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Fabrication of Yb³⁺-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum

Fabrication of Yb³⁺-Immobilized Hydrophilic Phytic-Acid-Coated Magnetic Nanocomposites for the Selective Separation of Bovine Hemoglobin from Bovine Serum

Fabrication of Yolk–Shell Fe₃O₄@NiSiO₃/Ni Microspheres for Efficient Purification of Histidine-Rich Proteins