Preparation of melanin-silver nanocomposites material and its physiological activity in vitro

Gao, Li; Guo, Lixiao; Zhang, Shuli; Zhao, Yinghu; Xie, Jun; Liu, Linlin; Xu, Hongyu

doi:10.21203/rs.3.rs-1638328/v1

Cited by 2 publications

(1 citation statement)

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“…The method for determining the scavenging ability of ABTS free radicals was based on the previously described method [25], with some modi cations. Brie y, the equal amounts of liquid forms of composites were mixed with ABTS (7mM) and kept dark for further 10-30 minutes.…”

Section: Antioxidant Properties Of Compositementioning

confidence: 99%

Biomaterial composed of chitosan, riboflavin and hydroxyapatite for bone tissue regeneration

Gaweł,

Milan,

Zebrowski

et al. 2023

Preprint

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Bone tissue engineering is an ongoing field of research due to the existing of burning needs in restoration and reconstruction of damaged bone. Numerous studies have shown the development of the biomaterials based on the hydroxyapatite, major component of bones. Biomaterials engineering approaches involve using a combination of miscellaneous bioactive molecules which may promote cell proliferation, and thus, forming a scaffold with the environment which favor the regeneration process. Chitosan, naturally occurring biodegradable polymer, possess some essential features, i.e biodegradability, biocompatibility, and in solid phase good porosity, which may be contributed to promote cell adhesion. Moreover, doping the materials with other biocompounds, will create a unique and multifunctional scaffold useful in regenerative medicine. Riboflavin is an essential water-soluble vitamin, which participates in numerous biological process, such as transport, cell development and reproduction. Therefore, this study is focused on the manufacturing of the composite materials based on the hydroxyapatite, chitosan and riboflavin. Scanning electron microscopy showed the porosity of the composite biomaterial, important factor which can affect cell ingrowth and new bone formation. The infrared spectroscopy demonstrated chemical interlinking between hydroxyapatite and chitosan phases as well as no evidence for chemical interaction between RF and the CS-HAP scaffold. This may alter physical and chemical properties of the scaffold towards better performance in potential regenerative applications, particularly, when the matrix is supplemented with RF. Indeed, in vitro experiments showed that the riboflavin increased the cell proliferation and migration of the fibroblasts and osteosarcoma cells. Due to the urgent need of development of material with a potential to prevent of implant-associated infections, the antimicrobial and antioxidant activity of the composite were determined. The composite material showed the inhibitory effect on Staphylococcus aureus and exhibited higher antioxidant activity compare to pure chitosan. The antibacterial effect may be due to the generation of ROS level. Moreover, the riboflavin photochemical treatment with blue LED light enhanced the ROS level, which could be a more accessible and safe practice to treat the implant-associated infections. All things considered, incorporating riboflavin into the biocomposite scaffolds may accelerate new bone regeneration.

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Section: Antioxidant Properties Of Compositementioning

confidence: 99%

Biomaterial composed of chitosan, riboflavin and hydroxyapatite for bone tissue regeneration

Gaweł,

Milan,

Zebrowski

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Biomaterial composed of chitosan, riboflavin, and hydroxyapatite for bone tissue regeneration

Gaweł,

Milan,

Żebrowski

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Biomaterial engineering approaches involve using a combination of miscellaneous bioactive molecules which may promote cell proliferation and, thus, form a scaffold with the environment that favors the regeneration process. Chitosan, a naturally occurring biodegradable polymer, possess some essential features, i.e., biodegradability, biocompatibility, and in the solid phase good porosity, which may contribute to promote cell adhesion. Moreover, doping of the materials with other biocompounds will create a unique and multifunctional scaffold that will be useful in regenerative medicine. This study is focused on the manufacturing and characterization of composite materials based on chitosan, hydroxyapatite, and riboflavin. The resulting films were fabricated by the casting/solvent evaporation method. Morphological and spectroscopy analyses of the films revealed a porous structure and an interconnection between chitosan and apatite. The composite material showed an inhibitory effect on Staphylococcus aureus and exhibited higher antioxidant activity compared to pure chitosan. In vitro studies on riboflavin showed increased cell proliferation and migration of fibroblasts and osteosarcoma cells, thus demonstrating their potential for bone tissue engineering applications.

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Preparation of melanin-silver nanocomposites material and its physiological activity in vitro

Cited by 2 publications

References 0 publications

Biomaterial composed of chitosan, riboflavin and hydroxyapatite for bone tissue regeneration

Biomaterial composed of chitosan, riboflavin and hydroxyapatite for bone tissue regeneration

Biomaterial composed of chitosan, riboflavin, and hydroxyapatite for bone tissue regeneration

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