Preparation of micro-patterned surfaces of Si-N-O films and their influence on adhesion behavior of endothelial cells

Zheng, Nan; Yang, Ping; Wang, QiYi; Yang, Zirong; Huang, Nan

doi:10.1007/s11431-009-0288-9

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…It appears that the 22 mm width on the stripes is smaller than the size scale of the macrophages. Our observation of more favorable cell attachment and increased spreading on the patterned surface of hyaluronic acid/glass and the rhodamine-dextran/hyaluronic acid is consistent with several cell studies on patterned surfaces involving cell lines such as fibroblasts, 44 epithelial cells, 45,46 endothelial cells 47,48 and macrophages. 11,49 This surface-directed cellular adhesion can serve as an excellent method to control inflammatory cell behavior for applications in tissue engineering, biomedical device development as well as diagnostic procedures and therapeutic interventions.…”

Section: Human Macrophage Adhesion Is Directed By Surface Micropatter...supporting

confidence: 92%

Human macrophage adhesion on polysaccharide patterned surfaces

et al. 2011

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Despite many advances in designing biocompatible materials, inflammation remains a problem in medical devices and implants. We report two methods, microcontact printing and photodegradation by UV exposure, to pattern dextran and hyaluronic acid on glass, as well as demonstrate their utility for use as an anti-inflammatory biomaterial. The dextran/glass patterned surface can be further modified by grafting hyaluronic acid to glass, creating a binary polysaccharide patterned surface. We used two geometries, 90 µm squares and 22 µm stripes, to study the human macrophage (THP-1) adhesion on the patterned surfaces containing dextran, hyaluronic acid and the binary pattern. The results indicate that a majority of the macrophages are non-adherent on hyaluronic acid for three day culture. The ranking of surfaces according to macrophage adhesion is 3-aminopropyl triethoxysilane-modified glass culture dish, dextranized surfaces, glass, and hyaluronic acid-modified surfaces. On the binary pattern of dextran and hyaluronic acid, macrophages preferentially attach and adhere to the dextranized area. Patterned surfaces provide an excellent platform for mimicking the complexity of the glycocalyx and investigating the interface between this surface and cells. This binary polysaccharide pattern also offers a new route to address anti-inflammatory potential of surface coatings on biomaterials in a high through-put fashion.

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Section: Human Macrophage Adhesion Is Directed By Surface Micropatter...supporting

confidence: 92%

Human macrophage adhesion on polysaccharide patterned surfaces

et al. 2011

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Surface patterning of a novel PEG‐functionalized poly‐l‐lactide polymer to improve its biocompatibility: Applications to bioresorbable vascular stents

et al. 2018

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Today, research in the field of bioresorbable vascular stents (BVS) not only focusses on a new material being nontoxic but also tries to enhance its biocompatibility in terms of endothelialization potential and hemocompatibility. To this end, we used picosecond laser ablation technology as a single-step and contactless method for surface microstructuring of a bioresorbable polymer which can be utilized in stent manufacture. The method works on all materials via fast material removal, can be easily adapted for micropatterning of tubular or more complex sample shapes and scaled up by means of micropatterning of metal molds for manufacturing. Here, picosecond laser ablation was applied to a bioresorbable, biologically inactive and polyethylene glycol-modified poly-L-lactide polymer (PEGylated PLLA) to generate parallel microgrooves with varying geometries. The different patterns were thoroughly evaluated by a series of cyto- and hemocompatibility tests revealing that all surfaces were non-toxic and non-hemolytic. More importantly, patterns with 20 to 25 µm wide and 6 to 7 µm deep grooves significantly enhanced endothelial cell adhesion in comparison to samples with smaller grooves. Here, human cardiac microvascular endothelial cells were found to align along the groove direction, which is thought to encourage endothelialization of intraluminal surfaces of BVS. © 2018 The Authors Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 00B: 000-000, 2018.

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Preparation of micro-patterned surfaces of Si-N-O films and their influence on adhesion behavior of endothelial cells

Cited by 2 publications

References 23 publications

Human macrophage adhesion on polysaccharide patterned surfaces

Human macrophage adhesion on polysaccharide patterned surfaces

Surface patterning of a novel PEG‐functionalized poly‐l‐lactide polymer to improve its biocompatibility: Applications to bioresorbable vascular stents

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