Preparation of molecularly imprinted Fe₃O₄/P(St‐DVB) composite beads with magnetic susceptibility and their characteristics of molecular recognition for amino acid

Abstract

Tyrosine and phenylalanine imprinted Fe 3 O 4 /P(St-DVB) composite beads with magnetic susceptibility were prepared by suspension polymerization using Fe 3 O 4 as the magnetically susceptible component, methacrylic acid and acrylamide as functional monomers, styrene and divinylbenzene as polymeric matrix components, stearic acid as porogen, and poly(ethylene glycol) 4000 as dispersant. Scanning electron microscopy examination of the composite beads showed macropores on the surface of spherical beads. The diameters of the composite beads and the macropores were in the ranges ϳ 400 -450 and 4 -20 m, respectively. The average content of Fe 3 O 4 inside the composite beads was 3.78%, and Fe 3 O 4 was unevenly distributed. The mechanism of macropore formation and the concept of "intellectual cavity" of molecularly imprinted composite beads were proposed. The recognition selectivity of the composite beads was investigated using tyrosine and phenylalanine as both templates and comparative molecules. Tyrosine-imprinted composite beads exhibited a good recognition selectivity for tyrosine, and the separation factor was up to 3.67. In contrast, phenylalanineimprinted composite beads had little recognition selectivity for phenylalanine and the separation factor was only 1.12. It was confirmed that the three-site interaction between tyrosine and functional monomers was stronger than the twosite interaction between phenylalanine and functional monomers.

Protein‐imprinted soft‐wet gel composite microspheres with magnetic susceptibility. II. Characteristics

“…To MIPs endowed with magnetically susceptible components, their magnetic responsibilities to external magnetic fields are important parameters too. 21 In the accompanying paper, 22 protein-imprinted soft-wet gel composite microspheres with magnetic susceptibility (MS-PIGMs) have been prepared by inverse suspension polymerization (ISP) using Fe 3 O 4 particles as magnetically susceptible component, acrylamide (AM) and N,NЈ-methylenebisacrylamide (BisAM) as polymeric matrix components, and bovine serum albumin (BSA, the resulting MS-PIGMs being named BSA-PIGMs) and lysozyme (Lyz, the resulting MS-PIGMs being named Lyz-PIGMs) as templates, respectively. The preparation and particle morphology of MS-PIGMs have been demonstrated in detail.…”

Preparation of D-phenylalanine imprinted microbeads by the modified suspension polymerization using a magnetic impeller

“…However, the molecularly imprinted polymers (MIP) prepared by the conventional bulk polymerization method produce irregularly shaped particles after crushing and sieving processes [3,4]. The particles are characterized by a large variation in size along with huge amounts of fine waste [5][6][7].…”

Preparation of molecularly imprinted polymers using theanine as dummy template and its application as SPE sorbent for the determination of eighteen amino acids in tobacco

“…Then, molecularly imprinted polymers of amino acids were prepared using various methods: precipitation polymerization, bulk polymerization, suspension polymerization, surface polymerization, multistep swelling suspension polymerization, magnetic polymer surface polymerization and sol-gel polymerization [18][19][20][21][22][23][24][25][26][27]. Although numerous studies were conducted to develop an effective molecular imprinting technology, the main challenges to their successful and widespread use are the tedious synthetic steps, template leakage, low binding capacity, and slow mass transfer of MIPs [28].…”