2007
DOI: 10.1089/hyb.2006.050
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Preparation of Monoclonal Antibodies Against Insulin and Their Applications

Abstract: Monoclonal antibodies (MAbs) against insulin are useful for insulin assays because of their specificity and plentiful supply. We have produced four monoclonal cell strains stably secreting the monoclonal antibodies against insulin; further established subpopulation, titer, affinity; and identified the antibodies as being of subclass IgG(2b)(kappa), one strain, or IgG(1)(kappa), three strains. The smallest detectable level of human insulin by ELISA using this IgG(1) was 1.25 microg/L. The monoclonal antibodies … Show more

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Cited by 3 publications
(2 citation statements)
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“…Monoclonal antibody was produced according to the hybridoma technology (Zhao et al . ). Briefly, BALB/c mouse was subcutaneously injected with 0·1 mg SEM in an emulsion of 10 mmol l −1 PBS and complete Freund's adjuvant (1 : 1 volume), then 0·05 mg SEM in Freund's incomplete adjuvant was immunized on the 28th, 42th, and 56th day, respectively.…”
Section: Methodsmentioning
confidence: 97%
“…Monoclonal antibody was produced according to the hybridoma technology (Zhao et al . ). Briefly, BALB/c mouse was subcutaneously injected with 0·1 mg SEM in an emulsion of 10 mmol l −1 PBS and complete Freund's adjuvant (1 : 1 volume), then 0·05 mg SEM in Freund's incomplete adjuvant was immunized on the 28th, 42th, and 56th day, respectively.…”
Section: Methodsmentioning
confidence: 97%
“…Monoclonal antibodies (MAbs) to SElI were generated from SElI-immunized BALB/c mice as previously described (Zhao et al 2007). In brief, 0Á1 mg SElI was subcutaneously injected into BALB/c mouse in an emulsion of PBS and complete Freund's adjuvant (1 : 1 volume), then 0Á05 mg SElI with Freund's incomplete adjuvant on the 28th, 42th and 56th day respectively.…”
Section: Monoclonal Antibody Productionmentioning
confidence: 99%