Preparation of monodispersed spectrally encoded microspheres with three different types of fluorophores

Shao, Yongbo; Chen, Hong; Zhang, Wei; Zhao, Yi-Jia; Fan, Li‐Juan

doi:10.1007/s10853-016-0651-8

Cited by 5 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the preparation of PGMA microspheres, four fluorescent molecules (FITC, RhB, PhB and SR101) were fed into the mixture of monomer, crosslinking reagent and initiator to synthesize the fluorescent FPGMA microspheres. During the polymerization process, the fluorescent molecules were incorporated and fixed stably in the FPGMA microspheres by the inclusion of FPGMA polymer chains and the affinity force including hydrogen-bond interaction, Van der Waals force and other multiple interactions between functional groups (epoxide groups and carbonyl groups) in PGMA and the active groups from the fluorescent molecules including hydroxyl, carbonyl, amino and sulfonic acid groups [8,13,33]. Single-fluorescent FPGMA microspheres doped with FITC, RhB, PhB and SR101 dyes were prepared.…”

Section: Results and Discussion 31 Synthesis And Characterization Omentioning

confidence: 99%

“…The key problem of the suspension array is the preparation of reliable and stable encoded microspheres, which determines the encoding signal, capacity and various chemical properties of the microspheres. Current encoding methods of microspheres include spectral encoding [7,8], chemical encoding [9,10], physical encoding [11,12] and pattern encoding [13]. As a promising encoding method, spectral encoding includes fluorescence encoding [14,15], Raman spectra encoding [16,17], and photonic-crystal encoding [4,18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Controllable preparation and high properties of fluorescence and surface enhanced raman spectra encoded poly(glycidyl methacrylate) microsphere

You¹,

Song²,

Huang³

et al. 2019

Express Polym. Lett.

View full text Add to dashboard Cite

Novel fluorescence and SERS encoded microspheres based on poly(glycidyl methacrylate) (PGMA) microspheres were developed by a controllable approach. The microspheres were encoded by four fluorescence dyes and three surface enhanced Raman spectra (SERS) probes. Multiple dyes could be simultaneously incorporated into the PGMA microspheres for encoding and the intensity of the fluorescence can be tuned by adjusting the feeding ratio. The PGMA microspheres were coated by silver nanoparticles and encoded by single and multiple SERS reporters. The fluorescence and SERS joint encoded PGMA microspheres were fabricated. All the fluorescence encoding signals can be excited at 488 nm. The fluorescent and SERS signals of dual-mode microspheres could be obtained from two optical channels and non-overlapping of emission spectra was observed in these encoded microspheres, which greatly increased the encoding capacity. The as prepared fluorescence and SERS encoded microspheres possess stable and distinct spectral encoding signals, large encoding capacity.

show abstract

Section: Results and Discussion 31 Synthesis And Characterization Omentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Controllable preparation and high properties of fluorescence and surface enhanced raman spectra encoded poly(glycidyl methacrylate) microsphere

You¹,

Song²,

Huang³

et al. 2019

Express Polym. Lett.

View full text Add to dashboard Cite

show abstract

“…The carriers of fluorescent microspheres are mostly organic or inorganic polymer materials. Fluorescent microspheres can be divided into different carriers and fluorescent substances, such as inorganic/organic, inorganic/inorganic, and organic/organic fluorescent microspheres. − Inorganic/organic ones use inorganic materials as the carrier and fluorescent substances as organic compounds. Inorganic/inorganic ones usually use silica gel as the carrier, combined with inorganic fluorescent nanocrystals with active groups.…”

Section: Introductionmentioning

confidence: 99%

Europium(III)-Based Fluorescent Microspheres with Styrene Copolymerization toward an Enhanced Photoluminescence Performance

Liu

Cai

et al. 2022

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

Rare earth-based polystyrene (PS) fluorescent microspheres, which vigorously combine the merits of rare earth elements and polystyrene microspheres, hold great potential for applications in the state-of-the-art technology and tremendous efforts have been devoted to the photoluminescence field. Herein, a 5-acrylamido-1,10-phenanthroline (Aphen) and Eu(III)-based polystyrene fluorescent microsphere was developed via a facial copolymerization strategy. The optimal complexes of Eu(β-NTA) 3 (Aphen) and Eu(BFA) 3 (TPPO) 2 were fabricated as the luminescent materials. Specifically, Eu(β-NTA) 3 (Aphen)-PS was modified for the first time to enhance the photoluminescence property, which effectively integrated the Aphen with styrene monomer. Meanwhile, Eu(BFA) 3 (TPPO) 2 was encapsulated into PS microspheres for the realization of Eu(BFA) 3 (TPPO) 2 -PS via an ubiquitous embedding process. The fluorescent intensity of Eu(β-NTA) 3 (Aphen)-PS was notably reinforced along with an increasing concentration of the rare earth complexes. However, the fluorescence intensity of Eu(BFA) 3 (TPPO) 2 -PS was decreased when the concentration was 50 mg due to the saturated solubility of the complexes. The obtained copolymerization fluorescent microspheres could prevent the premature leakage of the fluorescent complexes in a natural environment and showed an outstanding photoluminescence efficiency.

show abstract

“…As another well-known fluorophore with narrow emissions, quantum dots (QDs) are more competent for spectral encoding in that (i) they have much higher quantum yield than that of conventional organic fluorophores, not to mention of upconverting nanocrystals; (ii) the size-tunable emissions greatly enrich the number of encoding units; (iii) the encoded multicolor can be excited with one single wavelength [12][13][14][15][16]. Many kinds of QD-encoded microspheres so far have been constructed for multiplexed detection [17,18]. The encoding procedures are commonly accomplished by embedding different-sized QDs into microparticles through either post-treatment approach [19,20] or in situ polymerization [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Facile synthesis of multifunctional nanoparticles encoded with quantum dots and magnetic nanoparticles: cell tagging and MRI

et al. 2019

View full text Add to dashboard Cite

In this study, fluorescence-encoded magnetic biocompatible nanoparticles (NPs) were constructed from CdSe@ZnS quantum dots (QDs) and Fe3O4 nanoparticles with a one-step reprecipitation-encapsulation method. The resultant hybrid NPs exhibit small size (∼130 nm in diameter), highly bright QDs, two-color emissions (green and red) under single-wavelength excitation, easy separation with a magnet and efficient cellular internalization. Energy transfer between the incorporated QDs was studied to better tailor the encoded fluorescence, and 11 barcodes were obtained by adjusting the ratio of green and red QDs. We used four sets of the barcodes to tag specific cancer cells (HepG2) as a proof-of-concept, and distinguished each set according to respective overlayed fluorescence images using laser confocal microscopy. Moreover, the incorporated Fe3O4 NPs endowed as-constructed optical barcode superparamagnetic property by T2-enhanced magnetic resonance effect with an r2 value of 145.25 s−1 mM−1 at 3 T. These results suggest that the multifunctional NPs are very promising for discriminating different cells and dual-modality imaging.

show abstract

Preparation of monodispersed spectrally encoded microspheres with three different types of fluorophores

Cited by 5 publications

References 18 publications

Controllable preparation and high properties of fluorescence and surface enhanced raman spectra encoded poly(glycidyl methacrylate) microsphere

Controllable preparation and high properties of fluorescence and surface enhanced raman spectra encoded poly(glycidyl methacrylate) microsphere

Europium(III)-Based Fluorescent Microspheres with Styrene Copolymerization toward an Enhanced Photoluminescence Performance

Facile synthesis of multifunctional nanoparticles encoded with quantum dots and magnetic nanoparticles: cell tagging and MRI

Contact Info

Product

Resources

About