Preparation of Mustard (Brassica juncea L.) Protein Isolate and Recovery of Phenolic Compounds by Ultrafiltration

Abstract

Mustard (Brassica juncea L.) seed meal is good source of protein (28-36%) and phenolic antioxidants like sinapic acid and sinapine. In this work an ultrafiltration-diafiltration-based technique is developed for simultaneous preparation of protein isolate and recovery of phenolic rich fraction from seed meal using a high shear rotating disk membrane module with emphasis on reduced membrane fouling effect. The yield of membrane process found less (31.4% on dry basis) compared to precipitation process (42% on dry basis), but a high purity of protein isolate (96%) with improved functional properties was obtained. The phenolic content in the protein was found to be reduced from 7.23 mg/g of protein to as low as 0.6 mg/g of protein in this process. The phenolic-acid-rich fraction recovered shows about 87.24% radical scavenging and 34.3% chelation capacity per 100 ppm concentration. So, this process can be commercially exploited for protein isolate and phenolic antioxidant-rich product preparation after proper scale up.

Production of low chlorogenic and caffeic acid containing sunflower meal protein isolate and its use in functional wheat bread making

“…Amino acid composition of the end product (bread) supplemented with the SMPI was analyzed according to the method described in (Das et al 2009). Before amino acid analysis, each bread sample was hydrolyzed in 6.0 N HCl.…”

Antioxidant Effects of Ground Mustard Seed in Model Sausage Type Product

“…It has been shown that more than 70% of total phenolic content is sinapin, the cholin ester of sinapic acid (Amarowicz et al, 1996;Das et al, 2009). There are several research papers highlighting the effectiveness of ground mustard seed or its extracts in preventing oxidation processes in raw and cooked chicken meat and meat products (Shahidi et al, 2007;Kumar and Tanwar, 2011), but only a few about pork samples (Saleemi et al, 1993;Mc Carthy et al, 2001).…”

Enzyme membrane reactor in isolation of antioxidative peptides from oil industry waste: A comparison with non-peptidic antioxidants

“…2 kDa and 1 kDa fractionations were performed in RDM. Details of membrane module methodologies are accessible in our previous study (Das, Bhattacharjee, & Ghosh, 2009b, 2009c. Specifications of the membranes used are presented in Table 1.…”