Preparation of Nano-HAp as Vectors for Targeting Delivery System

Abstract

Nanoparticles can be easily transported in vivo and can reach and attack cancer cells. The biodegradable hydroxyapatite(HAp) nanoparticles which strongly inhibited the proliferation of the cancer cell wrapped with degradable copolymer monomethoxy(polyethyleneglycol)-poly (lactide-coglycolide)-monomethoxy (poly-ethyleneglycol) (PELGE). The nanoparticle preparation method is a critical problem for small-sized particles. HAP-loaded nanoparticles(NP) were fabricated by using a double-emulsion system. Orthogonal design was applied to optimize the preparation technology on the basis of the single factor evaluation. The optimal conditions for preparation HAP-loaded nanoparticle was as follows: 20mg/ml was the concentration of PELGE, volume of inner-phase of HAp was 0.5ml(C=12.5mg/ml), the ratio of DCM/acetone was 3/2 and the concentration of PluronicÒ F68 (Poloxamer 188 NF) was 3%. The entrapment efficiency was >88% and particle size less than 500nm. The nanoparticles, as detected by transmission electron microscopy (TEM), have a smooth and spherical surface. The HAP could be loaded into PELGE copolymers. In this study , the HAP nanoparticle-polymer delivery system was established using PELGE polymers as wraping material.

In vitroevaluation of the genotoxicity of a family of novel MeO-PEG-poly(D,L-lactic-co-glycolic acid)-PEG-OMe triblock copolymer and PLGA nanoparticles

“…The triblock copolymers PELGE were synthesized as described in the literature [18,19]. The compositions of the polymers are summarized in table 1.…”

Thermodynamics analyse of the synthesizing of nano-size hydroxyapatite using Sol-gel method

“…As can be known from a i -PH figures, when the thickness of a po 3 -is the highest in the water solution, the PH value 4 under 373K is lower than that under 373K, while it's almost the same under 373K and 473K. The boiling point of water under ordinary pressure is 373K, so the reaction under 473K need autoclave.…”

“…due to its excellent capability of ion exchange, low threshold value of fluorescence inspire, temperature sensitivity and humidity sensitivity[ 1-2] . Farther more hydroxyapatite of nano-size has more powerful biological activity, and has the effect to restrain acquired immune deficiency syndrome and cancer of stomach [3][4]. Sol-gel method which needs simple process and equipments is a kind of new way to synthesize nano-size powder.…”

A preliminary study on MeO‐PEG‐PLGA‐PEG‐OMe nanoparticles as intravenous carriers

“…PELGE‐DHAQ nanoparticles (NPs) were formulated by double‐emulsion technique as described in previous reports 14, 15. The mixture of 1 mL of acetone/dichloromethane (3/2) with 10 mg of dissolved PELGE polymer materials and 15% (w/v) aqueous DHAQ were emulsified by probe sonication at 360 W for 30 s, which formed the primary emulsion (w/o).…”