Preparation of nanostructured PDMS film as flexible immunosensor for cortisol analysis in human sweat

Liu, Quan; Shi, Weishan; Tian, Lei; Su, Mengjie; Jiang, Mengyuan; Li, Jing; Gu, Hongchen; Yu, Chunmei

doi:10.1016/j.aca.2021.339010

Cited by 31 publications

(18 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These fluctuations affect the production of epinephrine and norepinephrine, which are related to blood sugar levels, immunity, and blood pressure [ 12 ]. The excessive fluctuations of these two compounds at an inappropriate time affect the stress conditions; cortisol is one of the hormones released with them [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

2022

View full text Add to dashboard Cite

As a steroid hormone, cortisol has a close relationship with the stress response, and therefore, can be used as a biomarker for early detection of stress. An electrochemical immunosensor is one of the most widely used methods to detect cortisol, with antibodies as its bioreceptor. Apart from conventional laboratory-based methods, the trend for cortisol detection has seemed to be exploiting antibodies and aptamers. Both can provide satisfactory performance with high selectivity and sensitivity, but they still face issues with their short shelf life. Molecularly imprinted polymers (MIPs) have been widely used to detect macro- and micro-molecules by forming artificial antibodies as bioreceptors. MIPs are an alternative to natural antibodies, which despite demonstrating high selectivity and a low degree of cross-reactivity, often also show a high sensitivity to the environment, leading to their denaturation. MIPs can be prepared with convenient and relatively affordable fabrication processes. They also have high durability in ambient conditions, a long shelf life, and the ability to detect cortisol molecules at a concentration as low as 2 ag/mL. By collecting data from the past five years, this review summarizes the antibody and aptamer-based amperometric sensors as well as the latest developments exploiting MIPs rather than antibodies. Lastly, factors that can improve MIPs performance and are expected to be developed in the future are also explained.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

2022

View full text Add to dashboard Cite

show abstract

“…c. A polydimethylsiloxane-based nanostructured immunosensor for the investigation of cortisol in human sweat was fabricated by Liu et al [87].…”

Section: Recent Advances In Nanotechnology-based Biosensorsmentioning

confidence: 99%

An Overview of the Synergy of Electrochemistry and Nanotechnology for Advancements in Sensing Applications

Bais¹

2023

Frontiers in Voltammetry

View full text Add to dashboard Cite

Electrochemical sensors have been widely employed in diverse domains of electrochemical analysis, biosensing, drug administration, healthcare, agriculture, and so on because of their special potential features that are closely related to their high selectivity, sensitivity and cycling stability. Various electrochemical techniques employed to transduct biological or chemical signal to electrical signal are voltammetry, conductometry, potentiometry and amperometry. Due to the high demand of global market and human interest in having a device to check the concentration of species in different samples that is simple and fast, researchers have been engaged in a fierce competition to design and build new sensors and biosensors in recent years. The performance of the sensors can be considerably improved by modifying the electrode surfaces using diverse nanomaterials. Further, electrochemical biosensors are promising diagnostic tools that can find biomarkers in bodily fluids including sweat, urine, blood or excrement. Nanoparticles have found propitious role in biosensors, because they aid in functions like immobilisation of molecules, catalysis in electrosynthesis, facilitation of electron transfer between electrodes and biomolecules and labelling of biomolecules. The advance in the research amalgamating electrochemistry and nanotechnology for electro (bio) sensing applications is the beginning of a promising future for mankind and global market.

show abstract

“…However, to integrate the developed immunoplatform in body-attachable devices, other detection schemes avoiding the need for washing due to the use of a passive liquid handling device, together with integration of sample collection and handling in the same platform is required. A flexible and stretchable cortisol immunosensor susceptible to be translated to wearable devices has been reported by Liu et al [30] To construct this immunoplatform, a multi-walled carbon nanotubes (MWCNTs) film was deposited on a PDMS substrate followed by electrochemical deposition of Au nanoparticles (AuNPs) on the MWCNTs surface to covalently immobilize anti-cortisol monoclonal antibody by EDC/NHS chemistry. Cortisol detection was carried out by DPV in the presence of Fe(CN) 6 3À /4À .…”

Section: Susanamentioning

confidence: 99%

Affinity‐Based Wearable Electrochemical Biosensors: Natural versus Biomimetic Receptors

et al. 2022

View full text Add to dashboard Cite

This review delves into the titanic research efforts carried out during the last years on affinity-based wearable electrochemical biosensors, using both natural (antibodies) and biomimetic (aptamers, peptides and molecular imprinted polymers) receptors. The rationale and application of selected representative strategies is critically discussed, ending with realistic and futuristic visions of the technical barriers, challenges and prospects in the development and adoption of these biodevices in daily routines to ensure well-being against known, unknown and unexpected threats.

show abstract

Preparation of nanostructured PDMS film as flexible immunosensor for cortisol analysis in human sweat

Cited by 31 publications

References 38 publications

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

An Overview of the Synergy of Electrochemistry and Nanotechnology for Advancements in Sensing Applications

Affinity‐Based Wearable Electrochemical Biosensors: Natural versus Biomimetic Receptors

Contact Info

Product

Resources

About