Preparation of Newcastle Disease Virus Hemagglutination-Inhibition Test Antigen

Beard, C. W.; Hopkins, S. R.; Hammond, Jack

doi:10.2307/1589182

Cited by 31 publications

(8 citation statements)

References 5 publications

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“…The HI tests were done by the beta-procedure in V bottom microplates with automated equipment (Canalco Autotiter II, Canalco, Inc., 5635 Fisher Lane, Rockville, MD) using 10 HA units of antigen. The HI antigen was prepared according to the procedure described by Beard et al (1975). Serial twofold dilutions of serum in antigen, starting at 1:5, were prepared using 50 fi\ per well.…”

Section: Methodsmentioning

confidence: 99%

Vaccination of Broiler Chicks from Breeder Flocks Immunized with a Live or Inactivated Oil Emulsion Newcastle Disease Vaccine ,

et al. 1982

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One-day-old chicks used in this study were from breeder flocks vaccinated with live (B1 and LaSota) or inactivated oil emulsion Newcastle disease (ND) vaccine. Chicks were vaccinated against ND by various procedures. The vaccination response was evaluated by hemagglutination-inhibition antibody titers and by challenge. Chicks from breeder flocks vaccinated with live virus vaccine had a geometric mean hemagglutination-inhibition antibody titer (GMT) for Newcastle of 7 (low maternal antibody titer) at 1 day of age, whereas those chickens derived from breeder flocks vaccinated with inactivated oil emulsion ND vaccine had a GMT of 84 (high maternal antibody titer). One-day-old chicks injected with live B1 vaccine were not immunized against ND regardless of breeder flock source. However, chickens with low maternal antibody titers were effectively immunized against ND when injected at 1 day of age with an inactivated or inactivated plus live ND vaccine. Chicks with high maternal antibody titers were not effectively protected when vaccinated with inactivated vaccine at 1 day of age; however, these chicks were protected when injected with a combined live and inactivated ND vaccine. Chicks from both breeder flocks were effectively immunized against ND when injected at 1 day of age with a live or inactivated ND vaccine and revaccinated by aerosol at 21 days of age with live B1 ND vaccine. Even though they were protected against ND, there appears to have been an interference phenomenon in chicks derived from breeder flocks vaccinated with the live ND vaccine. Beak-O-Vac vaccinated chickens were not effectively protected against ND when compared with chicks vaccinated by aerosol at 1 day of age. Water vaccination at 7, 14, or 21 days of age was as effective as aerosol vaccination when administered to chicks with low maternal antibody titers. However, water vaccination was not as effective as aerosol vaccination when administered to chicks with high maternal antibody titers.

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Section: Methodsmentioning

confidence: 99%

Vaccination of Broiler Chicks from Breeder Flocks Immunized with a Live or Inactivated Oil Emulsion Newcastle Disease Vaccine ,

et al. 1982

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“…NDV antigen was prepared from allantoic fluid according to the method described by Beard et al [5]. Briefly, the NDV La Sota strain was grown in specific pathogen-free (SPF) embryonated chicken eggs, which were incubated at 37℃ for 4 days.…”

Section: Methodsmentioning

confidence: 99%

“…The hemagglutination inhibition (HI) assay is a commonly used immunoassay for the detection of NDV antibodies in poultry in many laboratories worldwide. The antigens used in the HI assay are prepared from live whole viruses or viruses killed with formalin or beta-propiolactone after the propagation of NDV in chicken embryonated eggs [1,5]. The HI assay is based on the detection of NDV antibodies that block the binding of chicken RBCs to the HN protein of NDV.…”

Section: Introductionmentioning

confidence: 99%

Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus

et al. 2013

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A recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from infected cells was used as an antigen in a hemagglutination inhibition (HI) test for the detection and titration of NDV-specific antibodies present in chicken sera. The rHN antigen produced high HA titers of 213 per 25 µL, which were similar to those of the NDV antigen produced using chicken eggs, and it remained stable without significant loss of the HA activity for at least 12 weeks at 4℃. The rHN-based HI assay specifically detected NDV antibodies, but not the sera of other avian pathogens, with a specificity and sensitivity of 100% and 98.0%, respectively, in known positive and negative chicken sera (n = 430). Compared with an NDV-based HI assay, the rHN-based HI assay had a relative sensitivity and specificity of 96.1% and 95.5%, respectively, when applied to field chicken sera. The HI titers of the rHN-based HI assay were highly correlated with those in an NDV-based HI assay (r = 0.927). Overall, these results indicate that rHN protein provides a useful alternative to NDV antigen in HI assays.

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“…To prepare the antigens for the HI test, the laboratory strains, CBU085253 and CBU082954, were inactivated by the method mentioned in a previous study (Beard et al, 1975 In an effort to determine the appropriate isolate for the animal experiment and serology, one field strain, CBU085253, out of the seven APMV type 4 isolates was selected because the host avian species was verified and there was no co-infection.…”

Section: ) Hi Assaymentioning

confidence: 99%

Epidemiological Studies of Avian Paramyxovirus Type 4 and 6 in Commercial Chicken Flocks in Korea

Lee¹,

Koo²,

Jeon³

et al. 2013

Korean Journal of Poultry Science

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Avian paramyxovirus (APMV) type 4 and 6 were isolated during an avian influenza (AI) surveillance program of wild birds. This study also conducted experimental infection of wild-bird-origin APMV type 4 and 6 in specific pathogen free (SPF) chickens to study pathogenicity and transmission within domestic flocks. In addition, serological prevalence data of APMV type 4 and 6 in domestic fowls was conducted with chicken sera collected from 2007 to 2009 in order to understand infection status. The results of the animal experiment showed that APMV type 4 and 6 had the ability to infect chickens with sero-conversion and to transmit the virus from infected birds to contacted birds, but showed low pathogenicity. Serological tests revealed that APMV type 4 was widespread in the poultry industry, especially in layer flocks, but the positive rate for APMV type 6 was very low. This study concluded that wild bird-origin APMV type 4 and 6 could infect the chickens by inter-species transmission and the seroprevalence of APMV type 4 was quite high in Korean poultry. However, since almost all the chicken flocks had a high level of antibody titer against APMV type 1, there was possibility of cross reaction between APMV type 1 and 4, which made the interpretations more complicated. In order to understand infection status in the natural environment, additional study is necessary regarding the seroprevalence of APMV type 4 and 6 in the wild bird population.(Key words : avian paramyxovirus type 4 and 6, experimental infection, inter-species transmission, seroprevalence, commercial chicken flocks) † To whom correspondence should be addressed : moip@cbu.ac.kr

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Preparation of Newcastle Disease Virus Hemagglutination-Inhibition Test Antigen

Cited by 31 publications

References 5 publications

Vaccination of Broiler Chicks from Breeder Flocks Immunized with a Live or Inactivated Oil Emulsion Newcastle Disease Vaccine ,

Vaccination of Broiler Chicks from Breeder Flocks Immunized with a Live or Inactivated Oil Emulsion Newcastle Disease Vaccine ,

Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus

Epidemiological Studies of Avian Paramyxovirus Type 4 and 6 in Commercial Chicken Flocks in Korea

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