Preparation of Pd/Bacterial Cellulose Hybrid Nanofibers for Dopamine Detection

Abstract

Palladium nanoparticle-bacterial cellulose (PdBC) hybrid nanofibers were synthesized by in-situ chemical reduction method. The obtained PdBC nanofibers were characterized by a series of analytical techniques. The results revealed that Pd nanoparticles were evenly dispersed on the surfaces of BC nanofibers. Then, the as-prepared PdBC nanofibers were mixed with laccase (Lac) and Nafion to obtain mixture suspension, which was further modified on electrode surface to construct novel biosensing platform. Finally, the prepared electrochemical biosensor was employed to detect dopamine. The analysis result was satisfactory, the sensor showed excellent electrocatalysis towards dopamine with high sensitivity (38.4 µA¨mM´1), low detection limit (1.26 µM), and wide linear range (5-167 µM). Moreover, the biosensor also showed good repeatability, reproducibility, selectivity and stability and was successfully used in the detection of dopamine in human urine, thus providing a promising method for dopamine analysis in clinical application.

Physicochemical characterization of high-quality bacterial cellulose produced by Komagataeibacter sp. strain W1 and identification of the associated genes in bacterial cellulose production

“…Furthermore, the peaks at 1162, 1107 and 1055 cm À1 can be assigned to C-C stretching vibration, skeletal vibration and ring vibration, respectively. 48 Besides, we also found four peaks at 400-1000 cm À1 (Fig. 6A).…”

Bacterial cellulose‐based biosensors

“…(Yang et al, 2009). Li, Ao, et al (2016) also reported the use of a similar in situ chemical-reduction method to develop BC-Pd biosensors.…”

“…In recent years, the use of nano-and biomaterials has triggered the development of novel sensing devices with high reaction rate and sensitivity. These new biosensors are being used to detect F I G U R E 8 Evaluation of the response of the BC-based biosensor prepared by Li, Ao, et al (2016) for the detection of dopamine in fluids with different interferents, including uric acid, ascorbic acid, levodopa, carbidopa and adrenaline. The good selectivity of this biosensor would make it useful to detect dopamine in human urine (reproduced from Li, Sun, et al, 2016, with…”

“…Table 1 summarizes the different materials that have been used to prepare BC‐based biosensors. Gold (Au), palladium (Pd) and platinum (Pt) nanoparticles, and titanium dioxide (TiO 2 ), ferrous oxide (FeO) and Zinc oxide (ZnO) have been used to increase the electric and magnetic conductivity of native BC (Gutierrez, Tercjak, Algar, Retegi, & Mondragon, 2012; Li, Ao, Wang, Lv, & Wei, 2016; Li, Sun, et al, 2016; Núñez‐Carmona et al, 2019; Sourty, Ryan, & Marchessault, 1998; Wang et al., 2010; Wang et al., 2011; Zhang et al, 2010). Polystyrene sulphonate (PEDOT) and polyaniline (PAni) have also been used to increase the conductivity of BC for the fabrication of biosensors (Di Pasquale, Graziani, Pollicino, & Trigona, 2019; Jasim, Ullah, Shi, Lin, & Yang, 2017; Hu, Chen, Liu, Ding, & Wang, 2011; Hu, Chen, Yang, Liu, & Wang, 2011; Huang & Gu, 2011; Huang, Virji, Weiller, & Kaner, 2003; Yue et al., 2017).…”

“…List of BC-based biosensors classified according to their transducing mechanism, sensing target and application nM/(30 nM-100 nM)Polluted water environmentsLi, Sun, et al (2016) BC-Pd NPs-Lac Electrochemical (Amperometry) Dopamine 1.26 µM/(5 µM-167 µM)Clinical applicationLi, Ao, et al (2016) BC-Au NPs-haem proteins Electrochemical…”