Preparation of photocured azidophenyl‐fish gelatin and its capturing of human epidermal growth factor on titanium plate

Park, Shin‐Hye; Seo, Si‐Yoong; Kang, Jeonghwa; Ito, Yoshihiro; Son, Tae‐Il

doi:10.1002/app.37854

Cited by 22 publications

(10 citation statements)

References 18 publications

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“…The present study demonstrates the applicability of using an f-gelatin and HA based bioink for the biofabrication of cellular sheets geared towards tissue engineering applications. The f-gelatin consisted mainly of porcine gelatin, modified by the incorporation of a furfural group [23,24]. Earlier studies on this f-gelatin led to the development of a scaffold for the treatment of in vivo osteochondral injuries and defects [15].…”

Section: Discussionmentioning

confidence: 99%

“…Furfuryl-gelatin (f-gelatin) was made as described below and contributed by our collaborators Drs. Park and Ito from RIKEN, Japan [24,36]. HA Sodium Salt (mol wt.…”

Section: Methodsmentioning

confidence: 99%

“…For biofabrication, a BIOBOT 1 (Biobots, Philadelphia, PA, USA) was used for which we formulated the bioink mixture, as described. A mixture of f-gelatin and HA was made, to which either RB or RF was added, to crosslink the bioprinted structure via visible lightinduced crosslinking mechanism [15,24]. For making 1 ml of this bioink solution, HA (10 mg, 1% w/v) was dissolved in DI water (900 μl, 25°C), followed by the addition and mixing of f-gelatin (100 mg, 10% w/v) at 25°C and then the mixture was heated at 37°C for 1 hr with stirring to facilitate the formation of a homogeneous viscous mixture.…”

Section: Biofabricationmentioning

confidence: 99%

“…Furfuryl-gelatin (f-gelatin) had no significant toxicity as it was shown to enhance cell migration and adhesion in vitro, in prior studies [23]. In addition, f-gelatin was also shown to be capable of protein immobilization for modification of existing implant surfaces [24] and for in vivo tissue repair applications [15]. In this study, our objective was to determine if an f-gelatin based bioink could be used for bioprinting [25], and for biofabrication of single and double layered cell sheets for tissue engineering [26].…”

Section: Introductionmentioning

confidence: 99%

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The applicability of furfuryl‐gelatin as a novel bioink for tissue engineering applications

et al. 2018

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Three-dimensional bioprinting is an innovative technique in tissue engineering, to create layer-by-layer structures, required for mimicking body tissues. However, synthetic bioinks do not generally possess high printability and biocompatibility at the same time. So, there is an urgent need for naturally derived bioinks that can exhibit such optimized properties. We used furfuryl-gelatin as a novel, visible-light crosslinkable bioink for fabricating cell-laden structures with high viability. Hyaluronic acid was added as a viscosity enhancer and either Rose Bengal or Riboflavin was used as a visible-light crosslinker. Crosslinking was done by exposing the printed structure for 2.5 min to visible light and confirmed using Fourier transform infrared spectroscopy and rheometry. Scanning electron microscopy revealed a highly porous networked structure. Three different cell types were successfully bioprinted within these constructs. Mouse mesenchymal stem cells printed within monolayer and bilayer sheets showed viability, network formation and proliferation (∼5.33 times) within 72 h of culture. C2C12 and STO cells were used to print a double layered structure, which showed evidence of the viability of both cells and heterocellular clusters within the construct. This furfuryl-gelatin based bioink can be used for tissue engineering of complex tissues and help in understanding how cellular crosstalk happens in vivo during normal or diseased pathology. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.

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Section: Discussionmentioning

confidence: 99%

“…Furfuryl-gelatin (f-gelatin) was made as described below and contributed by our collaborators Drs. Park and Ito from RIKEN, Japan [24,36]. HA Sodium Salt (mol wt.…”

Section: Methodsmentioning

confidence: 99%

Section: Biofabricationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The applicability of furfuryl‐gelatin as a novel bioink for tissue engineering applications

et al. 2018

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“…Furfuryl gelatin (f-gelatin), used as a basis for the “bioink”, was synthesized and characterized as described [15,16]. Hyaluronic Acid Sodium Salt (HA; mol.…”

Section: Methodsmentioning

confidence: 99%

A Comparative Study of a 3D Bioprinted Gelatin-Based Lattice and Rectangular-Sheet Structures

Kumar

Tasnim

Dominguez

et al. 2018

Gels

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3D bioprinting holds great promise in the field of regenerative medicine as it can create complex structures in a layer-by-layer manner using cell-laden bioinks, making it possible to imitate native tissues. Current bioinks lack both high printability and biocompatibility required in this respect. Hence, the development of bioinks that exhibit both properties is needed. In our previous study, a furfuryl-gelatin-based bioink, crosslinkable by visible light, was used for creating mouse mesenchymal stem cell-laden structures with a high fidelity. In this study, lattice mesh geometries were printed in a comparative study to test against the properties of a traditional rectangular-sheet. After 3D printing and crosslinking, both structures were analysed for swelling and rheological properties, and their porosity was estimated using scanning electron microscopy. The results showed that the lattice structure was relatively more porous with enhanced rheological properties and exhibited a lower degradation rate compared to the rectangular-sheet. Further, the lattice allowed cells to proliferate to a greater extent compared to the rectangular-sheet, which initially retained a lower number of cells. All of these results collectively affirmed that the lattice poses as a superior scaffold design for tissue engineering applications.

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Design and Synthesis of Photoreactive Polymers for Biomedical Applications

Sivakumar¹,

Zhou²,

Son³

et al. 2013

Biomimetics

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Preparation of photocured azidophenyl‐fish gelatin and its capturing of human epidermal growth factor on titanium plate

Cited by 22 publications

References 18 publications

The applicability of furfuryl‐gelatin as a novel bioink for tissue engineering applications

The applicability of furfuryl‐gelatin as a novel bioink for tissue engineering applications

A Comparative Study of a 3D Bioprinted Gelatin-Based Lattice and Rectangular-Sheet Structures

Design and Synthesis of Photoreactive Polymers for Biomedical Applications

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