Preparation of poly(N‐vinylpyrrolidone‐co‐pentaerythritol triacrylate) monolithic column for hydrophilic interaction chromatography

Abstract

A method for the preparation of poly(N-vinylpyrrolidone-co-pentaerythritol triacrylate copolymerization)-based monolithic capillary column was reported for the separation of polar small molecular weight compounds with nano-liquid chromatography in hydrophilic interaction chromatography mode. The monolithic columns were prepared by in situ copolymerization of N-vinylpyrrolidone and a cross-linker pentaerythritol triacrylate in a binary porogenic agent consisting of methanol and water. The composition of the polymerization solution was systematically optimized in terms of column permeability, theoretical plate number, asymmetric factor, and retention factor. A typical hydrophilic chromatography retention mechanism was observed with a mobile phase composed of a high content of organic solvent. The preparation method is simple and robust, the precursor N-vinylpyrrolidone is chemically stable, cheap, and easily available. The N-vinylpyrrolidone-based hydrophilic interaction chromatography stationary phase displays satisfactory separation selectivity for a range of polar test analytes, including benzoic acid derivatives, nucleosides, and phenols.

Comparing the Kinetic Hydrate Inhibition Performance of Linear versus Branched Polymers

“… 46 Trimethylolpropane triacrylate (TMP-3A), pentaerythritol tetraacrylate (PET-4A), and bis-pentaerythritol hexaacrylate (PET-6A) are well-known polymer cross-linkers used in several industrial applications that can give branched polymers at the correct monomer:cross-linker ratio ( Figure 3 ). 47 , 48 However, to the best of our knowledge, these cross-linkers have not been used to make branched KHI polymers. Here, we report the first study of branched versus linear PVCap using these three cross-linkers to form polymers with essentially 3, 4, and 6 branches.…”