Preparation of poly(<scp>DL</scp>‐lactide‐<i>co</i>‐glycolide) nanoparticles without surfactant

Jeong, Young‐Il; Cho, Chong‐Su; Kim, Sung-Hyun; Ko, K; Kim, Sunil; Shim, Yong-Ho; Nah, Jae‐Woon

doi:10.1002/app.1326

Cited by 80 publications

(28 citation statements)

References 25 publications

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“…Although the mechanism is not fully understood at present, it is thought that the principle of PLGA nanoparticles formation by the dialysis procedure may be based on a mechanism similar to the Fessi et al method, that is, by subsequent hydrophobic interactions among the polymer chains [6]. …”

Section: Resultsmentioning

confidence: 99%

Poly(hydroxyalkanoates)‐Based Polymeric Nanoparticles for Drug Delivery

Errico

Bartoli

Chiellini

et al. 2009

BioMed Research International

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Poly (hydroxyalkanoates) (PHAs) have recently attracted a great deal of academic and industrial interest for their biodegradability and biocompatibility making them suitable for environmental and biomedical applications. Poly(3-hydroxybutyrate-) (PHB-) and Poly(DL-lactide-co-glycolide) (PLGA-) based nanoparticles were prepared using the dialysis method as yet unreported for the preparation of nanoparticles based on PHB. Processing conditions were varied in order to evaluate their influence on morphology, drug encapsulation, and size of nanoparticles. The relevant results obtained give a theoretical understanding of the phenomenon occurring during colloidal formation. The adopted procedure allows for a relatively small diameter and homogeneity in size distribution of the PHB nanoparticles to be obtained compared to other methods like the one based on solvent evaporation which leads to particles on microscale. The biocompatibility of PHB and relative nanoparticles was investigated and both exhibited very good cytocompatibility.

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Section: Resultsmentioning

confidence: 99%

Poly(hydroxyalkanoates)‐Based Polymeric Nanoparticles for Drug Delivery

Errico

Bartoli

Chiellini

et al. 2009

BioMed Research International

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“…Since degradation is triggered by water that hydrolyzes the functional groups 30. Polydispersity index ( M w/ M n) is nearly unchanged, indicating that random chain cleavage is the likely degradation mechanism 40. On the other hand, the NPs with BCDen group degrade faster than those with CDen group.…”

Section: Resultsmentioning

confidence: 99%

Conjugates of poly(DL‐lactide‐co‐glycolide) on amino cyclodextrins and their nanoparticles as protein delivery system

Gao

Wang

Fan

et al. 2006

J Biomedical Materials Res

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Poly(DL-lactide-co-glycolide) (PLG) was chemically conjugated on two amino cyclodextrins, mono(6-(2-aminoethyl)amino-6-deoxy)-b-cyclodextrin and ethylenediamino bridged bis(b-cyclodextrin), to afford novel amphiphilic conjugates. Those conjugates were then characterized with infrared spectrometry (IR), proton nuclear magnetic resonance ( 1 H NMR) and gel permeation chromatography (GPC). A repeat-nanoprecipitation (RP-NP) method was also developed to fabricate the nanoparticles of the conjugates with a water-soluble model protein, bovine serum albumin (BSA). At the end of RP-NP process, the availability of BSA was over 80% while the entrapment efficiency was 40-50% for each nanoprecipitation. The nanoparticles were rigid and spherical with diameters of 110-180 nm determined by transmission electron microscope (TEM), atomic force microscopy (AFM) and particle size analyzer. Nanoparticles possessed good steric stability during freeze-drying and resuspensions due to the existence of cyclodextrins corona. Interactions between BSA and the conjugates in the nanoparticles were then elucidated with IR experiments. About 25% BSA adsorbed on the surface of nanoparticles due to the interaction and was easy to release in the first day. The release of BSA from the nanoparticles was in three phases: a burst effect in the first day, a followed plateau in about a week, and a sustained release of the protein over 14 days. By changing the lactide/glycolide ratio, the degradation time of the conjugates and the release rate of BSA could be controlled. The loss of CDs content was faster than that of overall Mw during degradation since CDs formed outer corona of the nanoparticles. Both the novel biomaterials and the nanosphere fabrication technique contributed to the maintenance of protein structure.

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“…Polyvinyl alcohol (PVA) is an uncharged and relatively hydrophilic polymer [9, 10] that has been widely used as a carrier polymer in drug delivery devices, such as sustained-release particles and hydrogels [11, 12], or as a surfactant for formulating polymeric drug carriers, such as the Lupron Depot ® [13, 14]. We observed that using PVA as a surfactant during particle formulation by the nanoprecipitation or emulsion methods leads to the formation of mucoadhesive nanoparticles [15–17].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle penetration of human cervicovaginal mucus: The effect of polyvinyl alcohol

Yang

Lai

et al. 2014

Journal of Controlled Release

107

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Therapeutic nanoparticles must rapidly penetrate the mucus secretions lining the surfaces of the respiratory, gastrointestinal and cervicovaginal tracts to efficiently reach the underlying tissues. Whereas most polymeric nanoparticles are highly mucoadhesive, we previously discovered that a dense layer of low MW polyethylene glycol (PEG) conferred a sufficiently hydrophilic and uncharged surface to effectively minimize mucin-nanoparticle adhesive interactions, allowing well-coated particles to rapidly diffuse through human mucus. Here, we sought to investigate the influence of surface coating by polyvinyl alcohol (PVA), a relatively hydrophilic and uncharged polymer routinely used as a surfactant to formulate drug carriers, on the transport of nanoparticles in fresh human cervicovaginal mucus. We found that PVA-coated polystyrene (PS) particles were immobilized, with speeds at least 4,000-fold lower in mucus than in water, regardless of the PVA molecular weight or incubation concentration tested. Nanoparticles composed of poly(lactide-co-glycolide) (PLGA) or diblock copolymers of PEG-PLGA were similarly immobilized when coated with PVA (slowed 29,000- and 2,500-fold, respectively). PVA coatings could not be adequately removed upon washing, and the residual PVA prevented sufficient coating with Pluronic F127 capable of reducing particle mucoadhesion. In contrast to PVA-coated particles, the similar sized PEG-coated formulations were slowed only ~6- to 10-fold in mucus compared to in water. Our results suggest incorporating PVA in the particle formulation process may lead to the formation of mucoadhesive particles for many nanoparticulate systems. Thus, alternative methods for particle formulation, based on novel surfactants or changes in the formulation process, should be identified and developed in order to produce mucus-penetrating particles for mucosal applications.

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Preparation of poly(DL‐lactide‐co‐glycolide) nanoparticles without surfactant

Cited by 80 publications

References 25 publications

Poly(hydroxyalkanoates)‐Based Polymeric Nanoparticles for Drug Delivery

Poly(hydroxyalkanoates)‐Based Polymeric Nanoparticles for Drug Delivery

Conjugates of poly(DL‐lactide‐co‐glycolide) on amino cyclodextrins and their nanoparticles as protein delivery system

Nanoparticle penetration of human cervicovaginal mucus: The effect of polyvinyl alcohol

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