Preparation of polydiacetylene immobilized optically encoded beads

Jun, Bong‐Hyun; Baek, Je Hyun; Kang, Homan; Park, Young June; Jeong, Dae Hong; Lee, Jun Young

doi:10.1016/j.jcis.2010.12.018

Cited by 13 publications

(17 citation statements)

References 33 publications

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“…In addition, Raman detection has high selectivity, since molecules have their own Raman fingerprint peaks (Gan et al, 2019). However, such applications of PDAs are still in the infancy, presumably due to the weak Raman signal caused by the small Raman cross sections area of C=C and C=C in PDA (Jun et al, 2011;Kim H.-M. et al, 2015).…”

Section: Pda For Raman Detection and Raman Bioimagingmentioning

confidence: 99%

Highly Sensitive Polydiacetylene Ensembles for Biosensing and Bioimaging

Huang

et al. 2020

Front. Chem.

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Section: Pda For Raman Detection and Raman Bioimagingmentioning

confidence: 99%

Highly Sensitive Polydiacetylene Ensembles for Biosensing and Bioimaging

Huang

et al. 2020

Front. Chem.

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“…30,65,126,151 However, some label-free patterns have also been developed. 202–204 Moreover, various patterns for signal amplification have been developed to increase overall sensitivity. 205–209 …”

Section: Optical Label and Signal Amplificationmentioning

confidence: 99%

“…204,235,236 As novel optoelectronic materials, CPs have been widely used in biosensing, bioimaging, therapy, 239–243 and barcoding 244 due to their high fluorescence quantum yields and large extinction coefficients. Jun et al 204 coated a polydiacetylene (PDA, a water-soluble fluorescent conjugated polymer 241 ) layer onto optically-encoded microspheres, with the PDA layer exhibiting a blue to red color change induced by external stress. However, color change of the PDA can be induced not only by antibody-antigen binding but also by other stressors, including pH and temperature, thus limiting their practical application for high-throughput, multiplexing of targets.…”

Section: Optical Label and Signal Amplificationmentioning

confidence: 99%

Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

et al. 2015

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Spectrometrically or optically encoded microsphere based suspension array technology (SAT) is applicable to the high-throughput, simultaneous detection of multiple analytes within a small, single sample volume. Thanks to the rapid development of nanotechnology, tremendous progress has been made in the multiplexed detecting capability, sensitivity, and photostability of suspension arrays. In this review, we first focus on the current stock of nanoparticle-based barcodes as well as the manufacturing technologies required for their production. We then move on to discuss all existing barcode-based bioanalysis patterns, including the various labels used in suspension arrays, label-free platforms, signal amplification methods, and fluorescence resonance energy transfer (FRET)-based platforms. We then introduce automatic platforms for suspension arrays that use superparamagnetic nanoparticle-based microspheres. Finally, we summarize the current challenges and their proposed solutions, which are centered on improving encoding capacities, alternative probe possibilities, nonspecificity suppression, directional immobilization, and “point of care” platforms. Throughout this review, we aim to provide a comprehensive guide for the design of suspension arrays, with the goal of improving their performance in areas such as multiplexing capacity, throughput, sensitivity, and cost effectiveness. We hope that our summary on the state-of-the-art development of these arrays, our commentary on future challenges, and some proposed avenues for further advances will help drive the development of suspension array technology and its related fields.

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“…Recently, we combined aptamer-based molecular beacons (MBs) [37] or polydiacetylene (PDA) [60] with optically-beads, which can be used with a bead-based array system. The system is designed to generate fluorescence by binding event as shown in Figure 3b, and offers the additional advantage of separation by target protein amount using flow cytometry.…”

Section: Fluorescence-encoded Beads For Protein Detectionmentioning

confidence: 99%

Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads

et al. 2012

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Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.

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Preparation of polydiacetylene immobilized optically encoded beads

Cited by 13 publications

References 33 publications

Highly Sensitive Polydiacetylene Ensembles for Biosensing and Bioimaging

Highly Sensitive Polydiacetylene Ensembles for Biosensing and Bioimaging

Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads

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