Preparation of silk sericin beads using LiCl/DMSO solvent and their potential as a drug carrier for oral administration

Preparation of Gel Film fromBombyx moriSilk Sericin and Its Characterization as a Wound Dressing

“…4) The Sericin Hope cocoon shells were dissolved in 8 M LiBr aqueous solution at 5 w/v% concentration at 35 C with stirring for 5 h. Sericin tends to aggregate at acidic pH because its isoelectric point is about 4. 24) To prevent aggregation of sericin in subsequent dialysis, a one-fourth volume of 0.5 M Gly-NaOH buffer (pH 9) was added to render the solution slightly basic (pH 7.5-8.0). After centrifugation (9;500 Â g, 5 min, 4 C), the supernatant was dialyzed using a membrane (Spectra/Por MWCO 12,000-14,000; Spectrum Laboratories, Rancho Dominguez, CA) against deionized water, and then against 0.1 mM sodium carbonate buffer (pH 8.8-9.2).…”

The Influence of Silkworm Species on Cellular Interactions with Novel PVA/Silk Sericin Hydrogels

“…Previous studies have produced sericin‐based gels via simply blending the sericin into gels,20 by ethanol treatment to induce gelation, or crystallinity of sericin10, 26, 27 or have integrated sericin with synthetic hydrogels using chemical crosslinkers such as glutaraldehyde 2, 17, 28. The problems with such approaches relate to the stability of gels in the former instances and the likelihood for chemical toxicity and regulatory concerns in the latter.…”

Effect of Lyoprotectant on the Solubility and Structure of Silk Sericin

“…However, several functional properties of sericin are reported and there were efforts to use sericin in cosmetics and biomedical fields (Kato et al, 1998;Oh et al, 2007;Ayumu et al, 2010;Hazeri et al, 2012). The hotwater extraction is the most simple and easiest method for the extraction of sericin (Oh et al, 2011).…”