Preparation of silver-decorated Soluplus® nanoparticles and antibacterial activity towards S. epidermidis biofilms as characterized by STEM-CL spectroscopy

Takahashi, Chisato; Yamada, T.; Yagi, Shunsuke; Murai, Takaaki; Muto, Shunsuke

doi:10.1016/j.msec.2020.111718

Cited by 10 publications

(2 citation statements)

References 44 publications

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“…These results indicate that SP can significantly reduce biofilm formation below the inhibitory concentration (25.8 mg/ml). This inhibitory effect of SP on biofilms has been described against Staphylococcus epidermidis when used as a polymeric nanocarrier (Takahashi et al, 2015;Albayaty et al, 2019;Takahashi et al, 2021). The amphiphilic nature of SP might contribute to this inhibition of biofilm formation as certain surfactants are known to weaken the biofilm matrix, causing biofilm disruption (Nguyen et al, 2020).…”

Section: Tested Excipients Had Significant Effects On P Aeruginosa Pr...mentioning

confidence: 86%

Formulation of water-soluble Buddleja globosa Hope extracts and characterization of their antimicrobial properties against Pseudomonas aeruginosa

Araya

Leiva-Soto²,

Bruna³

et al. 2022

Front. Pharmacol.

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Buddleja globosa Hope (BG) extracts are traditionally used to treat skin and gastric ulcers due to their healing properties. Non-aqueous solvents such as ethanol and DMSO are usually used to extract naturally occurring compounds. However, the cytotoxicity of these solvents and the low water solubility of the extracted compounds can hinder their biomedical applications. To overcome the limited solubility of the BG extracts, we aimed to enhance the solubility by processing a standardized hydroalcoholic extract (BG-126) through spray drying (SD), with and without two solubility enhancers. Spray-dried BG (BG-SD) extracts and spray-dried BG extracts plus polyvinylpyrrolidone (BG-SD PVP) and Soluplus® (BG-SD SP) were developed starting from BG-126 (containing 53% ethanol). These four formulations were characterized by total phenolic content, water solubility at 25°C and 37°C, and antimicrobial properties against Pseudomonas aeruginosa. All the SD formulations presented a solubility that allowed them to reach maximum concentrations of 1,024 μg/ml catechin for BG-SD and 2,048 μg/ml catechin for BG-SD PVP and BG-SD SP for antimicrobial testing. BG-SD showed the highest antimicrobial potency with a minimum inhibitory concentration (MIC) of 512 μg/ml catechin, followed by BG-126 with a MIC of 1,024 μg/ml catechin and SP. BG-126 was also shown to inhibit biofilm formation, as well as the excipients PVP and SP. The spray-dried BG (BG-SD) extract represents a promising natural active component with enhanced antimicrobial properties against P. aeruginosa for further research and the development of novel phytopharmaceuticals.

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Section: Tested Excipients Had Significant Effects On P Aeruginosa Pr...mentioning

confidence: 86%

Formulation of water-soluble Buddleja globosa Hope extracts and characterization of their antimicrobial properties against Pseudomonas aeruginosa

Araya

Leiva-Soto²,

Bruna³

et al. 2022

Front. Pharmacol.

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“…The Clt-NPs were found to have proper selectivity index values for clinical isolates of Candida spp. Similarly, Takahashi and his colleagues synthesized a silver-decorated PVCL-PVA-PEG graft copolymer that demonstrated high antibacterial activity as a drug delivery system effective against biofilm infections (Facchinatto et al, 2021;Takahashi et al, 2021). Considering all of these findings, there remains a gap in the development of a robust drug delivery system capable of loading temperature-sensitive hydrogel with polymeric micelles containing silver nanoparticles, and evaluating its therapeutic efficacy.…”

Section: Introductionmentioning

confidence: 99%

Nano drug delivery platform based on thermosensitive PEG-PCL hydrogel encapsulated in silver-bearing micelles and its antifungal activity investigation against vaginal candidiasis

2023

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Introduction: Vaginal candidiasis is a genital mucosal disease. Polymeric micelles (PMs) utilization due to improved solubility and low bioavailability of hydrophobic drugs e.g. clotrimazole is of interest. Silver nanoparticles could be applied to enhance antifungal properties. Loading PMs in injectable thermosensitive hydrogels by rapid distribution across vaginal walls improves the bioavailability of the drug and provides suitable therapeutic efficiency for drug delivery systems.Method: PCL-PEG-PCL (LGL) and PEG-PCL-PEG (GLG) copolymers were characterized by FTIR, HNMR, and GPC. Zeta potential and Size of synthesized PMs were determined by EMSA and DLS technics and morphological examination of PMs was conducted by FESEM and TEM. Silver-bearing polymeric micelles (PM-Ag) were characterized by DLS and LDE technics. EDX and UV-VIS spectroscopy confirmed silver nanoparticles' binding to PMs. Thermosensitive GLG hydrogel was considered a carrier for PM-Ag.Results: Encapsulation efficiency and drug loading content of micelles were calculated 64.53% and 14.6% respectively. The diameter and zeta potential of PMs were measured 166 ± 1.73 nm±SD and −6.26 ± 0.3 mV±SD and after silver-bearing it reached 197 ± 2.29 nm±SD and −5.38 ± 0.45 mV±SD respectively.Discussion: The biocompatibility of samples was investigated by MTT assay and the results indicated that up to a concentration of 125 µg/mL, the relative cell viability percentage exceeded 80%. Therefore, by considering the acceptable antifungal activity of the samples against C. Albicans, the designed drug delivery system is capable of sustained drug release over a specified period.

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pH‐sensitive size/surface charge‐adaptive quaternary ammonium salt copolymer micelles for antibiofilm activity against Staphylococcus aureus

Sun,

Fu,

Zhang

et al. 2024

J of Applied Polymer Sci

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The low penetration of antibacterial materials within biofilms results in a 1000‐fold decrease in bactericidal efficiency, complicating the complete removal of biofilms and potentially leading to persistent and recurrent bacterial infections that significantly impact human health. In this study, we present a multifunctional pH‐responsive antibacterial material based on random copolymers poly(dimethylaminoethyl methacrylate decyl ammonium bromide‐co‐polyethylene glycol methacrylate) copolymer (PQACs10‐co‐PEGMA10). The PQACs10‐co‐PEGMA10 copolymers could self‐assemble into micelles in selective solvent water. With the stealth function of polyethylene glycol (PEG), the PQACs10‐co‐PEGMA10 micelles can rapidly penetrate biofilms in a physiological environment and exhibit excellent antibacterial activity by exposing the quaternary ammonium salt (QACs) in an acidic microenvironment to eliminate biofilm. Furthermore, PQACs10‐co‐PEGMA10 micelles were applied as coatings using a dropwise method. The PEG chain formed hydration layer and the QACs chain for sterilization can hinders bacterial adhesion and proliferation, thereby preventing biofilm formation. The results show that the minimal inhibit concentration values and minimum biofilm eradication concentrations of the PQACs10‐co‐PEGMA10 micelles against Gram‐positive Staphylococcus aureus were 64 and 128 μg/mL, respectively. The antifouling and antibacterial rates of micelle coating against S. aureus were more than 99.99%. Taken together, the pH‐responsive PQACs10‐co‐PEGMA10 micelles demonstrate a good ability to clear and prevent biofilms, holding promise for complete biofilm removal and a reduction in biofilm‐related infections.

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Preparation of silver-decorated Soluplus® nanoparticles and antibacterial activity towards S. epidermidis biofilms as characterized by STEM-CL spectroscopy

Cited by 10 publications

References 44 publications

Formulation of water-soluble Buddleja globosa Hope extracts and characterization of their antimicrobial properties against Pseudomonas aeruginosa

Formulation of water-soluble Buddleja globosa Hope extracts and characterization of their antimicrobial properties against Pseudomonas aeruginosa

Nano drug delivery platform based on thermosensitive PEG-PCL hydrogel encapsulated in silver-bearing micelles and its antifungal activity investigation against vaginal candidiasis

pH‐sensitive size/surface charge‐adaptive quaternary ammonium salt copolymer micelles for antibiofilm activity against Staphylococcus aureus

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