Preparation of SiO<SUB>2</SUB>/(PMMA/Fe<SUB>3</SUB>O<SUB>4</SUB>) Nanoparticles Using Linolenic Acid as Crosslink Agent for Nucleic Acid Detection Using Chemiluminescent Method

Abstract

Usually, magnetic nanoparticles (MNPs) are prepared based on the famous Stöber process in which divinylbenzene (DVA) is often used as a crosslink agent to synthesize SiO2/(PMMA/Fe3O4) nanoparticles. Compared with DVA, linolenic acid (LNA) is innoxious and can polymerize more easily for it has three unsaturated double bonds. In this paper, LNA was used as a new crosslink agent instead of DVA to synthesize the SiO2/(PMMA/Fe3O4) nanoparticles. The results showed that the core-shell structure could be observed obviously. The sizes of nanoparticles with core-shell structure range from 200 to 500 nm. The DNA probes which was immobilized on the surface of MNPs were used to capture the biotin modified complementary sequence of the probe, and the formed complexes were bonded with streptavidin-modified alkaline phosphatase (SA-AP). Finally the chemiluminescent signals were detected by adding 3-(2'-spiroadamantane)-4-methoxy-4-(3"-phosphoryloxy) phenyl-1, 2-dioxetane (AMPPD) which was the substrate reagent of AP. The specificity and sensitivity of this approach were investigated in this paper.

High Throughput SNP Detection System Based on Magnetic Nanoparticles Separation

“…According to these key detection technology and detection strategies, including high throughput SNP genotyping method with dual-color fluorescence, 12 high throughput SNP genotyping based on solid-phase 13 14 high throughput SNP genotyping on gold magnetic nanoparticle array, 15 high throughput SNP genotyping using adaptor PCR, 16 high throughput immunological methods based on magnetic nanoparticles and chemiluminescent, [17][18][19][20][21][22][23] this system may complete all operations about the key detection technology, such as liquid pipetting, heating and cooling, fluorescence signal detection and so on. Furthermore it could realize full-automatic detection from sample extraction, target sequences amplification and purification and signal detection to result analysis.…”

Hg(II)-activated emission “turn-on” chemosensors excited by up-conversion nanocrystals: Synthesis, characterization and sensing performance

“…2 was synthesized by the following procedure [17]. Rhodamine 6G (2 g) and anhydrous hadrazine (10 mL) were dissolved in ethanol (30 mL) and heated to reflux under N 2 atmosphere for 12 h. Then the solution was cooled and poured into plenty of cold water.…”

An Automatic High-Throughput Single Nucleotide Polymorphism Genotyping Approach Based on Universal Tagged Arrays and Magnetic Nanoparticles

“…In recent years, magnetic nanoparticles have attracted a great deal of interest in various fields of advanced biological and medical applications, such as RNA and DNA purification, immunoassays, cell isolation, and DNA detection. [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42] Based on using magnetic nanoparticles as target DNA carrier, we have developed couple of genotyping methods previously which avoid any complex procedure including purification and concentration, [43][44] however, it still requires two types of labeled probes for each SNP locus, it increase the cost on the reagents when doing multiplexing analysis.…”