Preparation of Surface-Engineered Elastin/Lamin Nerve Guide Tubes of Poly(Lactic Acid)/Poly(Ethylene Vinyl Acetate)

Chandy, Thomas; Rao, Gundu H. R.

doi:10.1106/088391102026102

Cited by 4 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The composite ECM described in this paper is reconstituted from networks of alginate, elastin and PEG. Alginates, elastin and PEG matrices had been reported potentially useful for medical and pharmaceutical applications such as artificial skin, artificial kidney, cell encapsulation and as drug carriers for target delivery [9,16,19,21]. However, the development of a composite matrix from these polymers for tissue engineering has not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Elastin, the structural component of arterial wall, determines the arterial function and affects wall mechanical properties [18]. Currently, elastin is one of the ECM materials, being explored to obtain matrices with improved biomemetic and biomechanical properties of cell growth and immobilization [15,19]. Polyethylene glycol (PEG) is a water-soluble polymer that exhibits properties such as protein resistance, low toxicity and immunogenicity [20].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Development of Porous Alginate/Elastin/PEG Composite Matrix for Cardiovascular Engineering

Chandy¹,

Rao

Wilson³

et al. 2003

J Biomater Appl

Self Cite

View full text Add to dashboard Cite

The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue engineering and cell biology. To this end, gel matrices of different proportions of alginate/elastin/polyethylene glycol (Alg/Ela/PEG) were prepared and examined. The composite matrix membranes were evaluated for their porous scaffold using SEM, enzymatic degradation and water content. An equal blend of Alg/Ela with a ratio of Alg/Ela: PEG (7:3) was selected for fabricating Alg/Ela/PEG scaffolds for this study. The Alg/Ela/PEG membranes fabricated at 20 degrees C and - 20 degrees C had a mean surface pore size of 35-45 microm. However, their ultrastructures had shown bigger pore structures (60-75 microm) compared to their surface. It is interesting to note that the membranes of Alg/Ela/PEG prepared at 20 degrees C had larger ultrastructural pores than that of membranes prepared at -20 degrees C. Further, the SEM studies revealed that in the absence of PEG the composite membranes of Alg/Ela formed with less porous structures. The water content of membranes prepared at 20 degrees C was higher with Alg/Ela/PEG (61.6 +/- 4.8%), compared to Alg/Ela (49.9 +/- 0.3%). The enzymatic degradation and water content studies also revealed that the membranes fabricated at - 20 degrees C had high water uptake and low enzymatic degradation, as that of the membranes prepared at 20 degreees C. In other words the larger pore structured membranes had less water content and high degradation profile. This study proposes that this novel composite matrix produces a hierarchical structure that is useful for generating tissue scaffolds for repairing the failing cardiac muscles. However, more detailed investigations with cytocompatibility studies are needed to find applications.

show abstract

Section: Discussionmentioning

confidence: 99%