Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials

Shi, Caixin; Hou, Xinghui; Zhao, Dakui; Wang, Huili; Guo, Rong; Zhou, Ying

doi:10.1016/j.jmbbm.2021.105062

Cited by 26 publications

(15 citation statements)

References 66 publications

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“…Figure 3 shows the X‐ray diffraction patterns. The diffraction peak of CS was approximately 20°, the two main diffraction peaks of AL powder were approximately 14° and 22°, and the characteristic diffraction peak of CNT was approximately 25.9° 22 . However, there was only a broad peak in approximately 20°–30° for the composite scaffold, which may be a fused peak of each component.…”

Section: Resultsmentioning

confidence: 94%

“…The diffraction peak of CS was approximately 20 , the two main diffraction peaks of AL powder were approximately 14 and 22 , and the characteristic diffraction peak of CNT was approximately 25.9 . 22 However, there was only a broad peak in approximately 20 -30 for the composite scaffold, which may be a fused peak of each component. In addition, with increasing CNT concentrations, there were no significantly enhanced peak, which may be due to the low concentration of CNTs.…”

Section: Phase Compositionmentioning

confidence: 96%

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The improvement of periodontal tissue regeneration using a 3D‐printed carbon nanotube/chitosan/sodium alginate composite scaffold

Suo

Wang

et al. 2022

J Biomed Mater Res

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Periodontal disease is a common disease in the oral field, and many researchers are studying periodontal disease and try to find some biological scaffold materials to make periodontal tissue regenerative. In this study, we attempted to construct a carbon nanotube/chitosan/sodium alginate (CNT/CS/AL) ternary composite hydrogel and then prepare porous scaffold by 3D printing technology. Subsequently, characterizing the materials and testing the mechanical properties of the scaffold. Additionally, its effect on the proliferation of human periodontal ligament cells (hPDLCs) and its antibacterial effect on Porphyromonas gingivalis were detected. We found that CNT/CS/AL porous composite scaffolds with uniform pores could be successfully prepared. Moreover, with increasing CNT concentration, the degradation rate and the swelling degree of scaffold showed a downward trend. The compressive strength test indicated the elastic modulus of composite scaffolds ranged from 18 to 80 kPa, and 1% CNT/CS/AL group had the highest quantitative value. Subsequently, cell experiments showed that the CNT/CS/AL scaffold had good biocompatibility and could promote the proliferation of hPDLCs. Among 0.1%-1% CNT/CS/AL groups, the biocompatibility of 0.5% CNT/CS/AL scaffold performed best. Meanwhile, in vitro antibacterial experiments showed that the CNT/CS/AL scaffold had a certain bacteriostatic effect on P. gingivalis. When the concentration of CNT was more than 0.5%, the antimicrobial activity of composite scaffold was significantly promoted, and about 30% bacteria were inactivated. In conclusion, this 3D-printed CNT/CS/AL composite scaffold, with good material properties, biocompatibility and bacteriostatic activity, may be used for periodontal tissue regeneration, providing a new avenue for the treatment of periodontal disease.

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Section: Resultsmentioning

confidence: 94%

Section: Phase Compositionmentioning

confidence: 96%

The improvement of periodontal tissue regeneration using a 3D‐printed carbon nanotube/chitosan/sodium alginate composite scaffold

Suo

Wang

et al. 2022

J Biomed Mater Res

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“…46 The CSMP-MgO hydrogels in this study were prevented from swelling in DI water because the Mg(OH) 2 on the MgO NP surface would combine with protonated NH 3 + in CSMP solution, thus weakening the interaction between water and amino groups in CSMP and suppressing the penetration of water into the hydrogel network. For bone-grafting materials, mechanical properties, such as compressive properties, play an essential role in bone regeneration, 52 and similar mechanical performance to the implanted site would be desirable for the repair of bone defects. 53 In this CSMP-MgO injectable hydrogel system, the absence of fracture during compression might be attributed to the flexible combination of supramolecular combinations between MgO NPs and phosphate groups in CSMP.…”

Section: Discussionmentioning

confidence: 99%

Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

Chen

Sheng

Lin

et al. 2022

ACS Appl. Mater. Interfaces

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Natural polysaccharide (NPH)-based injectable hydrogels have shown great potential for critical-sized bone defect repair. However, their osteogenic, angiogenic, and mechanical properties are insufficient. Here, MgO nanoparticles (NPs) were incorporated into a newly synthesized water-soluble phosphocreatine-functionalized chitosan (CSMP) water solution to form an injectable hydrogel (CSMP-MgO) via supramolecular combination between phosphate groups in CSMP and magnesium in MgO NPs to circumvent these drawbacks of chitosan-based injectable hydrogels. Water-soluble chitosan deviate CSMP was first synthesized by grafting methacrylic anhydride and phosphocreatine into a chitosan chain in a one-step lyophilization process. The phosphocreatine in this hydrogel not only provides sites to combine with MgO NPs to form supramolecular binding but also serves as the reservoir to control Mg 2+ release. As a result, the lyophilized CSMP-MgO hydrogels presented a porous structure with some small holes in the pore wall, and the pore diameters ranged from 50 to 100 μm. The CSMP-MgO injectable hydrogels were restricted from swelling in DI water (lowest swelling ratio was 16.0 ± 1.1 g/g) and presented no brittle failure during compression even at a strain above 85% (maximum compressive strength was 195.0 kPa) versus the control groups (28.0 and 41.3 kPa for CSMP and CSMP-MgO (0.5) hydrogels), with regulated Mg 2+ release in a stable and sustained manner. The CSMP-MgO injectable hydrogels promoted in vitro calcium phosphate (hydroxyapatite (HA) and tetracalcium phosphate (TTCP)) deposition in supersaturated calcium phosphate solution and presented no cytotoxicity to MC3T3-E1 cells; the CSMP-MgO hydrogel promoted MC3T3-E1 cell osteogenic differentiation with upregulation of BSP, OPN, and Osterix osteogenic gene expression and mineralization and HUVEC tube formation. Among them, CSMP-MgO (5) presented most of these properties. Moreover, this hydrogel (CSMP-MgO ( 5)) showed an excellent ability to promote new bone formation in critical-sized calvarial defects in rats. Thus, the CSMP-MgO injectable hydrogel shows great promise for bone regeneration.

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“…Another composite of BAG is prepared with chitosan-alginate. A very recently published study reported that, with the increasing amount of sodium alginate in the composite, the mineralization ability of Bioglass was enhanced, and the composite's mechanical strength significantly increased (18). In addition, endogenous bone regeneration was determined by…”

Section: Summary Of Histopathological Examination Resultsmentioning

confidence: 99%

Can phosphatidylcholine increase the efficacy of bioactive glass graft when used as a carrier? an experimental study

Kaya

Karahan²,

Kurdal³

et al. 2022

Journal of Health Sciences and Medicine

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Aim: Bioactive glass (Bioglass) is a substance causing strong mechanical bondings at the interface of soft tissue-biomaterial-bone through a series of biochemical and biophysical reactions, commonly used to restore developing bone defects due to surgery. On the other hand, phosphatidylcholine is a lipid substance increasing antibiotics’ efficiency as a carrier. Since we met no study using the combination of Bioglass and phosphatidylcholine for bone defects, we aimed to investigate whether the bioglass-phosphatidylcholine combination would be more effective. Material and Method: Thirty Sprague-Dawley 3-6-months-old female rats with a mean weight of 400 gr were divided into five subgroups (six in each group). A 5-mm critical defect was created in the middle of the condyle throughout the burr’s diameter bilaterally. The phosphatidylcholine-bioglass graft was placed at one side, and Bioglass contralaterally to fill the defect. The rats were sacrificed at 24 hours, 72 hours, first, third, and sixth weeks postoperatively. The right and left rat femurs were removed and examined histopathologically. Results: There was no statistically significant difference between the groups regarding filling volume, newly formed and necrotic bone, fibrous tissue, residual graft material, integration, foreign body reaction, and defect organization, indicating that Bioglass served efficiently for filling the defect. In addition, phosphatidylcholine neither augmented nor impaired the healing process. Conclusion: These results indicated that Bioglass served efficiently for filling the defect, and the presence of phosphatidylcholine neither augmented nor impaired the healing process. However, further experimental studies are required until its clinical application is implemented.

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Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials

Cited by 26 publications

References 66 publications

The improvement of periodontal tissue regeneration using a 3D‐printed carbon nanotube/chitosan/sodium alginate composite scaffold

The improvement of periodontal tissue regeneration using a 3D‐printed carbon nanotube/chitosan/sodium alginate composite scaffold

Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

Can phosphatidylcholine increase the efficacy of bioactive glass graft when used as a carrier? an experimental study

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