Preparation of Ti4+-immobilized modified silica capillary trapping column for on-line selective enrichment of phosphopeptides

Selective Enrichment of Phosphopeptides and Phospholipids from Biological Matrixes on TiO2 Nanowire Arrays for Direct Molecular Characterization by Internal Extractive Electrospray Ionization Mass Spectrometry

“…Table S8 presents a comprehensive comparison of analytical performance for the proposed method with regard to the performance of previously reported MS methods ,,− based on titanium enrichment for the study of phosphopeptides and phospholipids. The data shows that the method established in this work is of higher speed, more sensitive for phospholipids, and with similar sensitivity for phosphopeptides compared with previously reported methods.…”

“…Typically, the TiO 2 particles or SPE cartridge filled with TiO 2 sorbent are used for the off-line enrichment of phosphopeptides and phospholipids prior to HPLC-MS analysis, ,,, requiring tedious sample preparations such as sample desalting, centrifugation, eluent drying, and chemical reconstitution, which greatly limits the speed of analysis for complex matrixes. Alternatively, online enrichment methods based on TiO 2 nanoparticle-deposited capillary column have also been developed, ,, but the sample flow rate in those methods should be maintained at a very low flow rate (e.g., 100 nL min –1 ) for the efficient trapping of phosphorylated peptides, thus resulting in a long time of analysis.…”

Dendritic Mesoporous Silica Nanoparticles with Abundant Ti4+ for Phosphopeptide Enrichment from Cancer Cells with 96% Specificity

“…DMSNs have a unique central-radial pore structure, large pore sizes, and highly accessible surface areas; thus, polymers can be easily modified onto the inner wall of the pores without significant pore blocking, thereby increasing the binding amount of chelating ligands. Simultaneously, the large pore size and short pore length can prevent the “shadow effect” and provide efficient mass transportation. , Polydopamine (PDA) with favorable hydrophilicity and biocompatibility possesses high cross-linking ability to metal ions. − The modification of PDA on DMSNs is expected to provide a new type of IMAC material for phosphopeptide enrichment with high performance.…”

An eco-friendly, low-cost, and automated strategy for phosphoproteome profiling

“…In the field of phosphoproteomics, automated, online, and eco-friendly analysis technology is expected to have broad appeal. Although ours is not the first investigation attempting online phosphoproteomic analysis, previous trials have not attained superior performance: some only implemented semi-automatic online analysis, 18,19 while others identified only a limited number of phosphorylation sites even with prior sample fractionation. 20,21 To our knowledge, there is no published report of automated online technology that can characterize thousands of phosphopeptides in a “single-shot” analysis.…”