Preparation, physicochemical and stability studies of chitosan-PNIPAM based responsive microgels under various pH and temperature conditions

Khan, Abbas; Othman, Muhammad Bisyrul Hafi; Chang, Boon Peng; Akil, Hazizan Md

doi:10.1007/s13726-015-0324-5

Cited by 27 publications

(17 citation statements)

References 48 publications

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“…The obtained samples were filtered with a 1 μm membrane filter and then dialyzed with a dialysis membrane (12,000 Da MWCO) against Milli-Q water, the water was changed periodically 15 times for 3 days. For long-term storage, nanogel was freeze-dried overnight (Echeverria et al, 2015 ; Khan et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Stimuli-Responsive, Plasmonic Nanogel for Dual Delivery of Curcumin and Photothermal Therapy for Cancer Treatment

et al. 2021

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Nanogels (Ng) are crosslinked polymer-based hydrogel nanoparticles considered to be next-generation drug delivery systems due to their superior properties, including high drug loading capacity, low toxicity, and stimuli responsiveness. In this study, dually thermo-pH-responsive plasmonic nanogel (AuNP@Ng) was synthesized by grafting poly (N-isopropyl acrylamide) (PNIPAM) to chitosan (CS) in the presence of a chemical crosslinker to serve as a drug carrier system. The nanogel was further incorporated with gold nanoparticles (AuNP) to provide simultaneous drug delivery and photothermal therapy (PTT). Curcumin's (Cur) low water solubility and low bioavailability are the biggest obstacles to effective use of curcumin for anticancer therapy, and these obstacles can be overcome by utilizing an efficient delivery system. Therefore, curcumin was chosen as a model drug to be loaded into the nanogel for enhancing the anticancer efficiency, and further, its therapeutic efficiency was enhanced by PTT of the formulated AuNP@Ng. Thorough characterization of Ng based on CS and PNIPAM was conducted to confirm successful synthesis. Furthermore, photothermal properties and swelling ratio of fabricated nanoparticles were evaluated. Morphology and size measurements of nanogel were determined by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Nanogel was found to have a hydrodynamic size of ~167 nm and exhibited sustained release of curcumin up to 72 h with dual thermo-pH responsive drug release behavior, as examined under different temperature and pH conditions. Cytocompatibility of plasmonic nanogel was evaluated on MDA-MB-231 human breast cancer and non-tumorigenic MCF 10A cell lines, and the findings indicated the nanogel formulation to be cytocompatible. Nanoparticle uptake studies showed high internalization of nanoparticles in cancer cells when compared with non-tumorigenic cells and confocal microscopy further demonstrated that AuNP@Ng were internalized into the MDA-MB-231 cancer cells via endosomal route. In vitro cytotoxicity studies revealed dose-dependent and time-dependent drug delivery of curcumin loaded AuNP@Ng/Cur. Furthermore, the developed nanoparticles showed an improved chemotherapy efficacy when irradiated with near-infrared (NIR) laser (808 nm) in vitro. This work revealed that synthesized plasmonic nanogel loaded with curcumin (AuNP@Ng/Cur) can act as stimuli-responsive nanocarriers, having potential for dual therapy i.e., delivery of hydrophobic drug and photothermal therapy.

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Section: Methodsmentioning

confidence: 99%

Stimuli-Responsive, Plasmonic Nanogel for Dual Delivery of Curcumin and Photothermal Therapy for Cancer Treatment

et al. 2021

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“…Firstly, the intervention of hydrophobic interactions between the acetyl groups of chitosan that, inducing aggregation, causes a size increase. 33,54 Second, a temperature-driven disruption of the network of hydrogen bonds between the polymer and the acids weakens the attractive interactions between the polymer chains and the MG swells. 55 Another crucial parameter that impacts the size increase of MGs with temperature before collapse is the time of reaction during their preparation.…”

Section: Resultsmentioning

confidence: 99%

“…This effect was very likely driven by two concurrent mechanisms, reported in the literature. Firstly, the intervention of hydrophobic interactions between the acetyl groups of chitosan that, inducing aggregation, causes a size increase 33,54 . Second, a temperature‐driven disruption of the network of hydrogen bonds between the polymer and the acids weakens the attractive interactions between the polymer chains and the MG swells 55 .…”

Section: Resultsmentioning

confidence: 99%

“…In particular, for drug delivery applications, copolymers of chitosan/N-isopropylmethacrylamide (Nipam) or N-isopropylmethacrylamide (Nipmam) have been studied as carriers for hydrophobic drugs. [31][32][33][34][35] Chitosan is a natural polymer obtained from the partial deacetylation of chitin. It consists of alternating monomers of 2-acetamido-2-deoxy-β-D-glucopyranose (GlcNAc) and 2-amino-2-deoxy-β-D-glucopyranose (GlcN).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, for drug delivery applications, copolymers of chitosan/N‐isopropylmethacrylamide (Nipam) or N‐isopropylmethacrylamide (Nipmam) have been studied as carriers for hydrophobic drugs 31–35 . Chitosan is a natural polymer obtained from the partial deacetylation of chitin.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stimuli‐responsive hybrid microgels for controlled drug delivery: Sorafenib as a model drug

Caputo

Aliberti

Cusano³

et al. 2020

J of Applied Polymer Sci

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Microgels (MGs) are synthetic colloidal hydrogel particles made of three dimensional polymer networks. Their chemical composition is crucial for their use as intelligent drug release systems operated by temperature control. Herein, several MGs using N-isopropylacrylamide (Nipam)/Nisopropylmethacrylamide (Nipmam), chitosan and acrylic/methacrylic acid have been synthesized by free radical polymerization reactions (NC MGs) and the effects of surfactants and different reaction times on size and swelling properties have been investigated. MGs have been identified and characterized by dynamic light scattering and atomic force microscopy, and finally used to optimize the encapsulation protocol of the hydrophobic drug sorafenib. The drug delivery system here described has encapsulation efficiency of 40% and releases 10% of the entrapped drug over about 16 h after the temperature is raised above the volume phase transition temperature. Data suggest that MGs with optimized composition may act as properly instructed entities able to trap and release biomolecules following external stimuli.

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Enzyme-free electrochemical detection of nanomolar levels of the organophosphorus pesticide paraoxon-ethyl by using a poly(N-isopropyl acrylamide)-chitosan microgel decorated with palladium nanoparticles

et al. 2019

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Preparation, physicochemical and stability studies of chitosan-PNIPAM based responsive microgels under various pH and temperature conditions

Cited by 27 publications

References 48 publications

Stimuli-Responsive, Plasmonic Nanogel for Dual Delivery of Curcumin and Photothermal Therapy for Cancer Treatment

Stimuli-Responsive, Plasmonic Nanogel for Dual Delivery of Curcumin and Photothermal Therapy for Cancer Treatment

Stimuli‐responsive hybrid microgels for controlled drug delivery: Sorafenib as a model drug

Enzyme-free electrochemical detection of nanomolar levels of the organophosphorus pesticide paraoxon-ethyl by using a poly(N-isopropyl acrylamide)-chitosan microgel decorated with palladium nanoparticles

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