Preparations of antibacterial yellow-green-fluorescent carbon dots and carbon dots-lysozyme complex and their applications in bacterial imaging and bacteria/biofilm inhibition/clearance

Zhao, Dan; Li, Xiaoyun; Xu, Mengyu; Jiao, Yan; Liu, Huan; Xiao, Xincai; Zhao, Hang

doi:10.1016/j.ijbiomac.2023.123303

Cited by 14 publications

(3 citation statements)

References 59 publications

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“…The lysozyme coupled form (CDs-LZM) of 4-ACDs showed good anti-bacterial activity (MIC = 5 mg ml À1 ) and biofilm inhibitory effect against S. aureus at 60 mg ml À1 , but for Gram-negative bacteria E. coli, their MIC value was significantly higher (120 mg ml À1 ). 33 However, our biogenic CDs showed improved anti-bacterial activity against E. coli (MIC = 1.25 mg ml À1 ) and both MDR strains of the biofilmforming Gram-negative P. aeruginosa (MIC = 2.5 mg ml À1 ) and E. cloacae (MIC = 25 mg ml À1 ).…”

Section: Minimum Inhibitory Concentration (Mic) and Minimum Bacterici...mentioning

confidence: 80%

“…In a recent study, Zhao et al , 2023 reported the broad-spectrum anti-bacterial effect of chemically synthesized CDs using 4-aminosalicylic acid and polyethylene imine (4-ACDs). 33 However, 4-ACDs were ineffective in eliminating S. aureus biofilm. The lysozyme coupled form (CDs-LZM) of 4-ACDs showed good anti-bacterial activity (MIC = 5 μg ml −1 ) and biofilm inhibitory effect against S. aureus at 60 μg ml −1 , but for Gram-negative bacteria E. coli , their MIC value was significantly higher (120 μg ml −1 ).…”

Section: Resultsmentioning

confidence: 99%

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Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Parambil,

Prasad,

Tomar

et al. 2024

J. Mater. Chem. B

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Section: Minimum Inhibitory Concentration (Mic) and Minimum Bacterici...mentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Parambil,

Prasad,

Tomar

et al. 2024

J. Mater. Chem. B

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“…CDs nanozymes with superoxide dismutase (SOD) activity provide new therapeutic ideas in the medical antimicrobial and anti-inflammatory areas . CDs are excellent antimicrobial drug candidates, and several antimicrobial active CDs have been developed by various research groups, including our team, but usually do not possess anti-inflammatory properties . Bacterial infections at the wound site are usually accompanied by an inflammatory response and the production of reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ), hydroxyl radicals (•OH), and superoxide anion radicals (O 2 •– ).…”

Section: Introductionmentioning

confidence: 99%

Gadolinium-Doped Carbon Dots with Dual Enzyme Activity for Visual Detection of Cu²⁺ and Antibacterial Applications

Zhao,

Zhang,

Liu

et al. 2024

ACS Appl. Nano Mater.

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The preparation and property studies of long-wavelength-emitting carbon dots (CDs) nanozymes with multifunctions like multienzyme activity nanozymes, antibacterial, and antioxidation have promising prospects in biochemical detection and clinical fields. This paper reports the fast preparation of dual enzyme-like active yellow-green carbon quantum dots (4-ASA/Gd-CDs) through a one-step hydrothermal route with the rare-earth element Gd doping, 4-aminosalicylic acid (4-ASA), and polyethyleneimine (PEI) as raw materials. The prepared CDs nanozymes can produce O 2•− under the irradiation of UV light and rapidly oxidize the colorless substrate o-phenylenediamine (OPD) into yellow 2,3-diaminophenazine (oxOPD); meanwhile, the heavy metal Cu 2+ can strongly coordinate with CDs, leading to electron transfer and the inhibition of CDs-like oxidative enzyme activity, and finally realize the real-time colorimetric detection to Cu 2+ . The limit of detection was as low as 0.38 μM (3σ/k) with a dynamic range from 0 to 38.4 μM. Moreover, the portable hydrogels (CDs-OPD-PSA) were also prepared, and the visual detection of Cu 2+ was achieved with the assistance of a smartphone. The experiment showed that the prepared 4-ASA/Gd-CDs nanozymes exhibited excellent antibacterial ability and superoxide dismutase (SOD)-like activity. The antibacterial mechanisms of CDs against gram-negative/positive bacteria were investigated through the potential value of 4-ASA/Gd-CDs and its own antioxidant activity. The MICs of 4-ASA/Gd-CDs against G + Staphylococcus aureus and Bacillus subtilis are 30 and 17 μg/mL, respectively, and those against G − Escherichia coli and Pseudomonas aeruginosa are 210 and 35 μg/mL, respectively. This research would provide an excellent theoretical and practical basis for the synthesis of dual enzyme-like active CDs and their application in antibacterial and antioxidation fields.

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Dual‐Carbon Dots Composite: A Multifunctional Photo‐Propelled Nanomotor Against Alzheimer's β‐Amyloid

Lin,

Dong,

Sun

2024

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The abnormal accumulation of β‐amyloid protein (Aβ) is considered as the main pathological hallmark of Alzheimer's disease (AD). The design of potent multifunctional theranostic agents targeting Aβ is one of the effective strategies for AD prevention and treatment. Nanomotors as intelligent, advanced, and multifunctional nanoplatforms can perform many complex tasks, but their application in AD theranostics is rare. Herein, sub‐10nm multifunctional dual‐carbon dots composites (ERCD) with photo‐propelled nanomotor behavior are fabricated by conjugating near‐infrared (NIR) carbon dots (RCD) of thermogenic and photodynamic capability with the previously reported epigallocatechin gallate‐derived carbonized polymer dots (ECD). ERCD‐1 (ECD:RCD = 1:2.5) showed potent inhibitory capability similar to ECD in the absence of NIR light, and exhibited photooxygenation activity and nanomotor behavior powered by “self‐thermophoretic force” under NIR irradiation, significantly enhancing the inhibition, disaggregation, and photooxygenation capabilities. The nanomotor suppressed Aβ fibrillization and rapidly disaggregated mature Aβ fibrils at very low concentrations (0.5 µg mL−1). Moreover, the NIR‐activated ERCD‐1 imaged Aβ plaques in vivo and prolonged nematode lifespan by 6 d at 2 µg mL−1. As a proof‐of‐concept, this work opened a new avenue to the design of multifunctional sub‐10nm nanomotors targeting Aβ for AD theranostics.

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Preparations of antibacterial yellow-green-fluorescent carbon dots and carbon dots-lysozyme complex and their applications in bacterial imaging and bacteria/biofilm inhibition/clearance

Cited by 14 publications

References 59 publications

Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Gadolinium-Doped Carbon Dots with Dual Enzyme Activity for Visual Detection of Cu²⁺ and Antibacterial Applications

Dual‐Carbon Dots Composite: A Multifunctional Photo‐Propelled Nanomotor Against Alzheimer's β‐Amyloid

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Preparations of antibacterial yellow-green-fluorescent carbon dots and carbon dots-lysozyme complex and their applications in bacterial imaging and bacteria/biofilm inhibition/clearance

Cited by 14 publications

References 59 publications

Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list

Gadolinium-Doped Carbon Dots with Dual Enzyme Activity for Visual Detection of Cu2+ and Antibacterial Applications

Dual‐Carbon Dots Composite: A Multifunctional Photo‐Propelled Nanomotor Against Alzheimer's β‐Amyloid

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Gadolinium-Doped Carbon Dots with Dual Enzyme Activity for Visual Detection of Cu²⁺ and Antibacterial Applications