Introduction Pathogenic bacteria, especially the ones with highly organized, systematic aggregating bacteria biofilm, would cause great harm to human health. The development of highly efficient antibacterial and antibiofilm functional fluorescent nanomaterial would be of great significance. Methods This paper reports the preparation of a series of antibacterial functional carbon dots (CDs) with chitosan (CS) and its derivatives as raw materials through one-step route, and the impact of various experiment parameters upon the optical properties and the antibacterial abilities have been explored, including the structures of the raw materials, excipients, and solvents. Results The CDs prepared by quaternary ammonium salt of chitosan (QCS) and ethylenediamine (EDA) exhibit multiple antibacterial effects through membrane breaking, DNA and protein destroying, and the production of singlet oxygen. The CDs showed excellent broad-spectrum inhibitory activity against a variety of bacteria (Gram-positive and negative bacteria), in particular, to the biofilm of Staphylococcus aureus with minimum inhibitory concentration at 10 µg/mL, showing great potential in killing bacteria and biofilms. The biocompatibility experiments proved that QCS-EDA-CDs are non-toxic to human normal hepatocytes and have low haemolytic effect. Furthermore, the prepared QCS-EDA-CDs have been successfully used in bacterial and biofilm imaging thanks to their excellent optical properties. Conclusion This paper explored the preparation and application of functional CDs, which can be used as the visual probe and therapeutic agents in the treatment of infections caused by bacteria and biofilm.

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Since biofilm infections could cause chronic diseases and drug resistance, posing serious threat to human health, the development of antibiofilm nanomaterials has attracted the interest of researchers. In this paper,...

Multifunctional carbon dots have drawn considerable attention due to their potential biomedical application value. We report the preparation of blue-green fluorescence-emitting, multifunctional, nitrogen-and-sulfur co-doped carbon dots (N, S-CDs) synthesized via a one-step process using 1-thioglycerol as a sulfur source, glucose and citric acid as carbon sources, and polyethyleneimine as a nitrogen source. Because of abundant amino and sulfur content, the CDs exhibited high sensibility and selectivity for detecting Cu2+ (detection limit: 0.01 μM, linear range: 0.025 to 50 μM). Fast and sensitive detection of tiopronin was also achieved on the basis of the fluorescence “off-on” mode considering the strong affinity between tiopronin and Cu2+. The N, S-CDs exhibited good biocompatibility as determined by fluorescence imaging using onion epidermal cells and gram-positive bacteria. The CDs also exhibited excellent antimicrobial ability against the gram-positive bacteria. Our results indicate that these novel N, S-CDs could be ideal candidates for several biochemical applications such as antibacterial treatment and detection of small biomolecules.