Preparations of the Tumor Necrosis Factor: Characteristics, Production Methods and Modification

Abstract

Tumor necrosis factor Recombinant drug (preparation) Producer Modification The paper presents data about the biological properties of the tumor necrosis factor (TNF) and drugs based on it, which are used in medical practice to combat cancer. The preparates for scientific research based on recombinant TNF have also been described. The brief information about the possibilities of obtaining TNF using unicellular pro-(Escherichia coli) and eukaryotes (Saccharomyces cerevisiae, Pichia pastoris) is given. The TNF synthesis inducers (bacterial lipopolysaccharides, phytohemaglutenin, antigens of various organisms) that are used in the cultivation of immunocompetent animals or human mononuclear cells are characterized. The possibilities of TNF modification to improve its biological properties, increase activity and reduce toxicity, create TNF nanocomposites with antibiotics, antibody fragments, and metal nanoparticles, disadvantages of the use of polyethylene glycol for modification of TNF have also been presented.