“…Previous work showed improved yields when performed in both batch and continuous reactors (Katoh and Katoh, 2000;Linke et al, 2014;Pan et al, 2014;Schlegl et al, 2005;Winter et al, 2002). Other strategies for improving yield and productivity include buffer optimization (Berg et al, 2012;Mannall et al, 2009;Ordidge et al, 2012), better mixing (Mannall et al, 2006), on-column refolding (Li et al, 2004;Schmoeger et al, 2009) and annular chromatography (Uretschlager and Jungbauer, 2002) However, to accurately quantify process performance and optimize process of these refolding strategies, a robust kinetic model that satisfies different reactor formats is beneficial (Buswell and Middelberg, 2003). Moreover, a correct biomolecular reaction scheme would help facilitate product quality and acceptable variability of process parameters by serving as a mechanistic model support tool in Process Analytical Technology (PAT) (Glassey et al, 2011) as part of the Quality by Design (QbD) concept (Rathore and Winkle, 2009) in biomanufacturing.…”