2002
DOI: 10.1007/s00449-002-0282-0
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Preparative continuous annular chromatography (P-CAC), a review

Abstract: Notation c liquid phase solute concentration (g/l) c* liquid concentration in equilibrium (g/l) D z axial dispersion coefficient (cm 2 /min) D m solute dispersion coefficient in the axial direction (cm 2 /min) D h solute dispersion coefficient in the angular direction (cm 2 /min) k 0 a global mass transfer parameter (s -1 ) k¢ A normalized retention of compound A k¢ B normalized retention of compound B K distribution coefficient K m mass transfer coefficient between the mobile and the stationary phases K eq eq… Show more

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Cited by 30 publications
(10 citation statements)
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“…The stability of the separation process over a longer period of time is a critical parameter for continuous processing [22]. The elution profiles at steady state were evaluated after 2, 6 and 15 hours of operation at a feed concentration of 1.044 mg/mL (see Fig.…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…The stability of the separation process over a longer period of time is a critical parameter for continuous processing [22]. The elution profiles at steady state were evaluated after 2, 6 and 15 hours of operation at a feed concentration of 1.044 mg/mL (see Fig.…”
Section: Resultsmentioning
confidence: 99%
“…All relevant chromatographic process parameters such as the selection of a proper stationary phase, column length and diameter, initial concentration of denatured protein, loading factor, regeneration and eluent buffer velocity can be optimized in small-scale discontinuous chromatography experiments and directly transferred to the PCAC system [16,17]. Optimization allows for the determination of elution positions and elution volumes for the various protein forms, i.e., native and aggregated forms.…”
Section: Continuous Separationmentioning
confidence: 99%
See 1 more Smart Citation
“…These systems provide periodic (cyclical) operation, with the product concentration in the eluent stream varying with time during each cycle. Annular [28] and radial [29] chromatography can also be operated in continuous mode with true steady-state operation, although neither of these have been implemented for large-scale commercial protein purification.…”
Section: Technologies For Continuous Bioprocessingmentioning
confidence: 99%
“…Previous work showed improved yields when performed in both batch and continuous reactors (Katoh and Katoh, 2000;Linke et al, 2014;Pan et al, 2014;Schlegl et al, 2005;Winter et al, 2002). Other strategies for improving yield and productivity include buffer optimization (Berg et al, 2012;Mannall et al, 2009;Ordidge et al, 2012), better mixing (Mannall et al, 2006), on-column refolding (Li et al, 2004;Schmoeger et al, 2009) and annular chromatography (Uretschlager and Jungbauer, 2002) However, to accurately quantify process performance and optimize process of these refolding strategies, a robust kinetic model that satisfies different reactor formats is beneficial (Buswell and Middelberg, 2003). Moreover, a correct biomolecular reaction scheme would help facilitate product quality and acceptable variability of process parameters by serving as a mechanistic model support tool in Process Analytical Technology (PAT) (Glassey et al, 2011) as part of the Quality by Design (QbD) concept (Rathore and Winkle, 2009) in biomanufacturing.…”
Section: Introductionmentioning
confidence: 96%