Preparative counter-current chromatography isolation of liensinine and its analogues from embryo of the seed of Nelumbo nucifera GAERTN. using upright coil planet centrifuge with four multilayer coils connected in series

Wu, Shihua; Sun, Cuirong; Cao, Xiaoji; Zhou, Hui; Zou, Hong; Pan, Yuanjiang

doi:10.1016/j.chroma.2004.05.003

Cited by 71 publications

(51 citation statements)

References 6 publications

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“…3 was carried out by 1 H NMR and 13 C NMR. Compared with the data given in reference [19], peak 1, peak 2 and peak 3 were identified as liensinine, isoliensinine and neferine, respectively (see Supporting Information).…”

Section: Identification Of Hsccc Peak Fractionsmentioning

confidence: 99%

“…It was reported that liensinine and its analogues could be isolated from the seed embryo of N. nucifera by countercurrent chromatography [19]. However, two kinds of twophase solvent systems composed of light petroleum-ethyl acetate -tetrachloromethane-chloroform-methanol-water and ethyl acetate-tetrachloromethane-methanol-water were rather complicated.…”

Section: Introductionmentioning

confidence: 99%

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Preparative separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN using high‐speed counter‐current chromatography

Shao

Wang

et al. 2009

J of Separation Science

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A preparative high-speed counter-current chromatography method for separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN was successfully established by using n-hexane-ethyl acetate-methanol-water (5:8:4:5, v/v, containing 0.5% NH(4)OH) as the two-phase solvent system. From 200 mg of crude extract, 18.4 mg of liensinine, 19.6 mg of isoliensinine and 58.4 mg of neferine were obtained with the purity of 96.8, 95.9, and 98.6%, respectively. The identification of the three alkaloids was performed with (1)H NMR and (13)C NMR.

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Section: Identification Of Hsccc Peak Fractionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparative separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN using high‐speed counter‐current chromatography

Shao

Wang

et al. 2009

J of Separation Science

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“…. Compared with the data given in references [11,21], compound C was identified as neferine. The structures of the three compounds were further confirmed by comparison with authentic samples.…”

Section: Identification Of the Isolated Compoundsmentioning

confidence: 99%

“…Using the conventional methods, such as column chromatography and TLC, it is very difficult to separate them because of their structural similarity [19,20]. A conventional HSCCC method for successfully separating the alkaloids from N. nucifera Gaertn with a self-designed upright coil planet centrifuge has been reported by Wu et al [21]. However, no Xiao Wang Jianhua Liu Yanling Geng Daijie Wang Hongjing Dong Tianyou Zhang report has been seen on the use of pH-zone-refining CCC for the separation of neferine and its analogues from plants.…”

Section: Introductionmentioning

confidence: 99%

Preparative separation of alkaloids from Nelumbo nucifera Gaertn by pH‐zone‐refining counter‐current chromatography

Wang

Liu

Geng

et al. 2010

J of Separation Science

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Preparative separation of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn has been successfully performed for the first time using pH-zone-refining counter-current chromatography. Two kinds of two-phase solvent systems were applied to preparative CCC isolation. The first was the system composed of MtBE-water (1:1, v/v), 10 mM triethylamine in organic stationary phase and 5 mM HCl in aqueous mobile phase, which could only separate neferine partly from the crude extract. The second was the system composed of n-hexane-ethyl acetate-methanol-water (5:5:2:8, v/v), 10 mM triethylamine in organic stationary phase and 5 mM HCl in aqueous mobile phase, which was the optimum for large-scale CCC isolation. 2.5 g of the crude alkaloid was purified in one-step separation of 7 h, yielding 151 mg of liensinine, 118 mg of isoliensinine and 572 mg of neferine with the purities of 93.0, 95.1 and 97.0%, respectively.

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“…India, Japan, Korea, South East Asia, Russia and some countries in Africa [3] . Both types of lotus leaves, aerial as well as floating are large, non-wettable and orbicular and 20-90 cm in diameter.…”

mentioning

confidence: 99%

Development and Validation of HPTLC Method for Quantitative Estimation of Nuciferine from Lotus leaves

Patel¹,

Jadhav²

2017

pharmaceutical-sciences

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Nafsin and Aruna: Detection and Quantification of Nuciferine by HPTLCNelumbo nucifera (Nelumbonaceae), commonly known as lotus, is an aquatic perennial plant. A simple, precise and accurate high-performance thin-layer chromatography method for quantitative estimation of an alkaloid, nuciferine from methanol extract of lotus leaf was developed. The method employed high-performance thinlayer chromatography aluminium plate precoated with silica gel 60F 254 (10×10 cm) as stationary phase and separation was achieved by using a suitable mobile phase chloroform:ethylacetate:methanol:glacial acetic acid [6.5:1:2.5:0.025 v/v/v/v] and Dragendorff's reagent was used for detection. Quantitation of the method was achieved by densitometric method at 272 nm using Camag TLC scanner 3. This method showed good separation of nuciferine in the extract with R f value 0.44±0.02. The regression analysis data indicated good linear relationship for the calibration plots for nuciferine in the range of 100-450 ng/spot and regression coefficient was 0.998. The accuracy and reliability of the proposed method was ascertained by evaluating various validation parameters like linearity, precision, accuracy, robustness, specificity, limit of quantification and limit of detection according to the ICH guideline. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of nuciferine. The developed high-performance thin-layer chromatography method can be used for quantification of nuciferine in different species of lotus and formulations containing nuciferine.Key words: High performance thin layer chromatography, lotus leaf, nuciferine, quantification, alkaloids *Address for correspondence E-mail: drarunajadhav@gmail.com Nelumbo nucifera is perennial, rhizomatous, aquatic plant belonging to the family of Nelumbonaceae, which has numerous common names (Indian lotus, Chinese water lily and sacred lotus) [1,2] . It is widely grown and consumed over the world, mainly in China,

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Preparative counter-current chromatography isolation of liensinine and its analogues from embryo of the seed of Nelumbo nucifera GAERTN. using upright coil planet centrifuge with four multilayer coils connected in series

Cited by 71 publications

References 6 publications

Preparative separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN using high‐speed counter‐current chromatography

Preparative separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN using high‐speed counter‐current chromatography

Preparative separation of alkaloids from Nelumbo nucifera Gaertn by pH‐zone‐refining counter‐current chromatography

Development and Validation of HPTLC Method for Quantitative Estimation of Nuciferine from Lotus leaves

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