Preparative enantioseparations using supercritical fluid chromatography

Supercritical fluid chromatography in pharmaceutical analysis

“…Due to the low viscosity of supercritical mobile phases, high mobile phase flow rates can be employed, leading to an improvement of productivity for SFC purifications, and a reduction of the time required to generate pure compounds for pharmaceutical testing [148]. Another advantage of preparative SFC over preparative LC is the lower organic solvent consumption, which is achieved by replacing a majority of the mobile phase with CO 2 .…”

Evaluation and comparison of three different separation techniques for analysis of retroamide enantiomers and their biological evaluation against h-P2X7 receptor

“…Therefore enantioselective preparative chromatography has appeared as the most efficient way to obtain simultaneously both enantiomers [8] in contrast to tedious asymmetric synthesis. Whereas HPLC has proven its efficiency for the preparative scale since the last twenty years, an ecological new technique, the supercritical fluid chromatography (SFC), was developed since the ten last years and was found particularly relevant for this preparative step [9][10][11][12]. Its main advantages (e.g.…”

Effects of supercritical fluid chromatography conditions on enantioselectivity and performance of polyproline-derived chiral stationary phases

“…Thus, higher flow rates can be used, which reduce analysis and equilibration times, and increases high-throughput capacity [19]. Moreover, SFC is increasingly applied to the separation of enantiomers and offers significant advantages especially for preparative purposes [20]. Among them, the separated enantiomers can be easily recovered after separation by evaporation of the low amount of co-solvent used.…”