Preparative enrichment of human tissue cells capable to change a site of growth <i>in vitro</i> or <i>in vivo</i> - Recent developments

Bauer, Johann; Cohly, Hari H. P.; Sahana, Jayashree; Grimm, Daniela

doi:10.1080/10826068.2018.1525567

Cited by 2 publications

(2 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Preliminary hints towards a µ g -dependent change of intracellular vinculin distribution had already been observed during a recent TEXUS flight [45,51]. Taking together, in vitro studies on thyroid cells exposed to microgravity suggested that groups of proteins are affected by µ g which interact over the membrane barrier and provided first insights in the mechanisms of tissue cells leaving the site of growth to migrate to another one as observed in metastasis [60].…”

Section: Research On Thyroid Cancer Cells In Microgravitymentioning

confidence: 96%

Fighting Thyroid Cancer with Microgravity Research

Krüger

Melnik

Kopp

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Microgravity in space or simulated by special ground-based devices provides an unusual but unique environment to study and influence tumour cell processes. By investigating thyroid cancer cells in microgravity for nearly 20 years, researchers got insights into tumour biology that had not been possible under normal laboratory conditions: adherently growing cancer cells detach from their surface and form three-dimensional structures. The cells included in these multicellular spheroids (MCS) were not only altered but behave also differently to those grown in flat sheets in normal gravity, more closely mimicking the conditions in the human body. Therefore, MCS became an invaluable model for studying metastasis and developing new cancer treatment strategies via drug targeting. Microgravity intervenes deeply in processes such as apoptosis and in structural changes involving the cytoskeleton and the extracellular matrix, which influence cell growth. Most interestingly, follicular thyroid cancer cells grown under microgravity conditions were shifted towards a less-malignant phenotype. Results from microgravity research can be used to rethink conventional cancer research and may help to pinpoint the cellular changes that cause cancer. This in turn could lead to novel therapies that will enhance the quality of life for patients or potentially develop new preventive countermeasures.

show abstract

Section: Research On Thyroid Cancer Cells In Microgravitymentioning

confidence: 96%

Fighting Thyroid Cancer with Microgravity Research

Krüger

Melnik

Kopp

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Until now, electrostatic units firmly bound to cell surfaces could be observed and quantitatively determined when singularized tissue cells were placed in an electric field of direct current [132,133]. It is known that the electrophoretic behavior of the cells depends on the amount of sialic acids bound to the cell surface [134], and similarities between the behavior of cells in an electric field and on an RPM have recently been reviewed [135]. In both cases, adhesion proteins played a major role.…”

Section: Biochemical and Biophysical Effects Of Sas Present On Extracmentioning

confidence: 99%

Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach

Bauer

Gombocz²,

Wehland

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

The adhesion behavior of human tissue cells changes in vitro, when gravity forces affecting these cells are modified. To understand the mechanisms underlying these changes, proteins involved in cell-cell or cell-extracellular matrix adhesion, their expression, accumulation, localization, and posttranslational modification (PTM) regarding changes during exposure to microgravity were investigated. As the sialylation of adhesion proteins is influencing cell adhesion on Earth in vitro and in vivo, we analyzed the sialylation of cell adhesion molecules detected by omics studies on cells, which change their adhesion behavior when exposed to microgravity. Using a knowledge graph created from experimental omics data and semantic searches across several reference databases, we studied the sialylation of adhesion proteins glycosylated at their extracellular domains with regards to its sensitivity to microgravity. This way, experimental omics data networked with the current knowledge about the binding of sialic acids to cell adhesion proteins, its regulation, and interactions in between those proteins provided insights into the mechanisms behind our experimental findings, suggesting that balancing the sialylation against the de-sialylation of the terminal ends of the adhesion proteins’ glycans influences their binding activity. This sheds light on the transition from two- to three-dimensional growth observed in microgravity, mirroring cell migration and cancer metastasis in vivo.

show abstract

Preparative enrichment of human tissue cells capable to change a site of growth in vitro or in vivo - Recent developments

Cited by 2 publications

References 71 publications

Fighting Thyroid Cancer with Microgravity Research

Fighting Thyroid Cancer with Microgravity Research

Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach

Contact Info

Product

Resources

About