Preparative ion-exchange chromatography of proteins from dairy whey

Ion-Exchange Chromatography and Its Applications

“…Therefore within the last decade there has also been increasing interest in the liquid chromatographic processes because of the growing pharmaceutical industry and needs from the pharmaceutical and specialty chemical industries for highly specific and efficient separation methods. Several different types of liquid chromatography techniques are utilized for isolation of bioactive molecules from different sources [25]. Ion exchange chromatography is probably the most powerful and classic type of liquid chromatography.…”

“…Analyte(s): α-lactalbumin, β-lactoglobulin, bovine serum albumin, immunoglobulin G and lactose [25]. Source: Lycium ruthenicum Murr.…”

Isolation and Purification of Bioactive Proteins from Bovine Colostrum

“…The chromatographic procedure involved sequentially pumping different solutions into the column: (1) equilibration (EQ) buffer to adjust column pH; (2) whey; (3) EQ buffer to rinse unbound material from the column; and (4) different elution buffers to selectively desorbed different bound proteins. The optimum conditions for initially separating the proteins such as -lactalbumin, -lactoglobulin, bovine serum albumin, immunoglobulin G and lactose from a sweet dairy whey mixture could be determined by a commercial anion-exchange resin (Gerberding & Byers, 1998). The separation was accomplished with simultaneous step elution changes in salt concentration and pH.…”

Enzyme Chemotaxis on Paper-based Devices

“…Chromatography and chromatographic techniques have for many years been a means to separate species in a variety of mixtures. [8][9][10][11][12][13] Paper has historically been the stationary phase of choice due to its cellulosic structure and ability to interact with molecules of varied types due to its fibers nature, thereby, creating a complex of theoretical plates. It also easily transports fluids via capillary action without the need of an external power source, and has a high surface area-to-volume ratio.…”