Preparative isolation and purification of macrolactin antibiotics from marine bacterium Bacillus amyloliquefaciens using high-speed counter-current chromatography in stepwise elution mode

He, Shan; Wang, Hongqiang; Yan, Xiaojun; Zhu, Peng; Chen, Juanjuan; Yang, Rui

doi:10.1016/j.chroma.2012.11.029

Cited by 41 publications

(30 citation statements)

References 20 publications

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“…By comparison of the NMR and MS spectroscopic data with those reported in the literature, 12 known compounds were then identified to be macrolactin A ( 2 ) [10], macrolactin F ( 3 ) [11], cyclo( d )-Pro-( d )-Leu ( 4 ) [12] cyclo-(Leu-Thr) ( 5 ) [13], thymidine ( 6 ) [14], 2′- O -methyluridine ( 7 ) [15], indol-3-carbaldehyde ( 8 ) [16], 2-(1 H -indol-3-yl)ethan-1-ol ( 9 ) [17], 2-hydroxy phenyl acetic acid ( 10 ) [18], 4-(hydroxymethyl)phenol ( 11 ) [15], 2-(4-hydroxyphenyl)-ethanol ( 12 ) [19], and 2,3-butanediol ( 13 ) [20]. …”

Section: Resultsmentioning

confidence: 99%

Anti-Allergic Compounds from the Deep-Sea-Derived Actinomycete Nesterenkonia flava MCCC 1K00610

et al. 2017

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A novel cyclic ether, nesterenkoniane (1), was isolated from the deep-sea-derived actinomycete Nesterenkonia flava MCCC 1K00610, together with 12 known compounds, including two macrolides (2, 3), two diketopiperazines (4, 5), two nucleosides (6, 7), two indoles (8, 9), three phenolics (10–12), and one butanol derivate (13). Their structures were established mainly on detailed analysis of the NMR and MS spectroscopic data. All 13 compounds were tested for anti-allergic activities using immunoglobulin E (IgE) mediated rat mast RBL-2H3 cell model. Under the concentration of 20 μg/mL, 1 exhibited moderate anti-allergic activity with inhibition rate of 9.86%, compared to that of 37.41% of the positive control, loratadine. While cyclo(d)-Pro-(d)-Leu (4) and indol-3-carbaldehyde (8) showed the most potent effects with the IC50 values of 69.95 and 57.12 μg/mL, respectively, which was comparable to that of loratadine (IC50 = 35.01 μg/mL). To the best of our knowledge, it is the first report on secondary metabolites from the genus of Nesterenkonia.

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Section: Resultsmentioning

confidence: 99%

Anti-Allergic Compounds from the Deep-Sea-Derived Actinomycete Nesterenkonia flava MCCC 1K00610

et al. 2017

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“…There is no commercial standard sample for sale. The standard sample was obtained using high-speed counter-current chromatography (HSCCC) method reported previously (He et al, 2012, 2013). The concentration of macrolactins in the collected solution was quantified to be 38.6 mg/L.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Compounds-Macrolactin Alters the Soil Bacterial Community and Abundance of the Gene Encoding PKS

Yuan

Zhao

et al. 2016

Front. Microbiol.

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Macrolactin produced by many soil microbes has been shown to be an efficient antibacterial agent against many bacterial pathogens. However, studies examining the effect of macrolactin on both the soil bacterial community and the intrinsic bacterial species that harbor genes responsible for the production of this antibiotic have not been conducted so far. In this study, a mixture of macrolactin was isolated from the liquid culture of Bacillus amyloliquefaciens NJN-6, and applied to the soil once a week for four weeks. 16S rRNA Illumina MiSeq sequencing showed that continuous application of macrolactin reduced the α-diversity of the soil bacterial community and thereby changed the relative abundance of microbes at both the phylum and genus level. The relative abundance of Proteobacteria and Firmicutes was significantly increased along with a significant decrease in the relative abundance of Acidobacteria. However, the application of macrolactins had an insignificant effect on the total numbers of bacteria. Further, the native gene responsible for the production of macrolactin, the gene encoding polyketide synthase was reduced in copy number after the application of macrolactin. The results of this study suggested that a bactericide from a microbial source could decrease the diversity of the soil bacterial community and change the bacterial community structure. Moreover, the populations of the intrinsic bacterial species which harbor genes responsible for macrolactin production were inhibited when the external source antibiotic was applied.

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“…Trong số đó, macrolatin A có hoạt tính kháng vi khuẩn mạnh nhất, đặc biệt là khả năng đối kháng với một số loại vi khuẩn kháng thuốc kháng sinh như Staphylococcus aureus kháng methicillin và Enterococcus kháng vancomycin (Romero-Tabarez et al, 2006;Kim et al, 2011). Macrolactin A đã được phát hiện trong dịch nuôi cấy của một số chủng B. velezensis như chủng GA1 (Arguelles-Arias et al, 2009) và ESB-2 (He et al, 2013). Macrolactin A sản sinh từ các chủng B. velezensis như HR62 và NJN-6 đã được chứng minh có hoạt tính kháng lại các loại vi khuẩn gây bệnh héo xanh Ralstonia solanacearum (Huang et al, 2014;Yuan et al, 2012).…”

Section: Sttunclassified

Extraction, purification and characterization of antifungal and antibacterial substances produced from Bacillus velezensis CP 1604

Trung

Vân

Liên³

et al. 2016

Vietnam J. Biotechnol.

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Bacillus velezensis is one of the members of the Bacillus subtilis species complex which is generally considered as safety organism. The bacterium possesses several beneficial properties for crops, especially production of antifungal and antibacterial agents against plant pathogens. In this study, we aimed to investigate the biological properties of antimicrobial subtances produced from B. velezensis CP 1604. From liquid culture, substances with activity against Fusarium oxysporum and Xanthomonas oryzae were extracted by means of adsorption with Amberlite XAD-7, extraction from lyophilized powder using ethanol, precipitation at low pH and extraction with organic solvents of 1-butanol and 2-pentanol. The substances were subsequently purified using High Performance Liquid Chromatography (HPLC). Antifungal substance was eluted at 5.328 min while antibacterial substance was observed at 15.313 min. Mass spectrometry analysis showed that antifungal substance was iturin A with molecular weight of 1042 Da and antibacterial substance was macrolactin A with molecular weight of 402 Da. The antifungal substance was stable at high temperature but antibacterial activity was significantly reduced when treated at 100° C for 2 hours. Both substances reduced the activity at low pH but the activities still maintained at high pH. The antimicrobial activities against the fungal and bacterium were not affected when treated with hydrolytic enzymes such as trypsin, α-chymotrypsin, amylase, lipase and proteinase K. Further safety and efficiency investigations of the antifungal and antibacterial substances from B. velezensis CP 1604 on the plant disease control are required in order to seek for the potential application on sustainable agricultural production.

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Preparative isolation and purification of macrolactin antibiotics from marine bacterium Bacillus amyloliquefaciens using high-speed counter-current chromatography in stepwise elution mode

Cited by 41 publications

References 20 publications

Anti-Allergic Compounds from the Deep-Sea-Derived Actinomycete Nesterenkonia flava MCCC 1K00610

Anti-Allergic Compounds from the Deep-Sea-Derived Actinomycete Nesterenkonia flava MCCC 1K00610

Antibacterial Compounds-Macrolactin Alters the Soil Bacterial Community and Abundance of the Gene Encoding PKS

Extraction, purification and characterization of antifungal and antibacterial substances produced from Bacillus velezensis CP 1604

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