Preparative isolation and purification of hydroxyanthraquinones and cinnamic acid from the Chinese medicinal herb Rheum officinale Baill. by high-speed counter-current chromatography

Liu, Renmin; Li, Aifeng; Sun, Ailing

doi:10.1016/j.chroma.2004.08.101

Cited by 103 publications

(45 citation statements)

References 11 publications

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“…Artifact formation is also eliminated, since it used no solid support. HSCCC has been used for analysis, separation and purification of bioactive compounds from natural products, such as alkaloids, hydroxyanthraquinones, flavonoids and saponins (Shibusawa et al, 2000;Liu et al, 2004;Aman et al, 2005;Chu et al, 2005;Li and Chen, 2005;Zhou et al, 2006;Gutzeit et al, 2007;Yao et al, 2007). This technique presents maximum capacity with an excellent sample recovery and high efficiency, and can be employed for preparative-scale separations in a straightforward manner (Tong et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high‐speed counter‐current chromatography

Assimopoulou

Sturm

Stuppner

et al. 2008

Biomedical Chromatography

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Alkannin and shikonin (A/S) and their derivatives have been found in the roots of several Boraginaceous species and are also produced through plant tissue cultures. The chiral compounds A/S are potent pharmaceutical substances with a wide spectrum of biological and pharmacological activities like wound healing, antimicrobial, anti-inflammatory, anticancer and antioxidant activity. High-speed counter-current chromatography (HSCCC) was applied for the first time to the separation, preparative isolation and purification of A/S and their esters from extracts of Alkanna tinctoria roots, as well as commercial samples. The constituents of HSCCC fractions and their purity were determined by high-performance liquid chromatographydiode array detection-mass spectrometry (HPLC-DAD-MS), since DAD cannot detect oligomeric A/S derivatives that are present in most of the samples containing the respective monomeric derivatives. The purity of HSCCC fractions was compared with the one of fractions isolated by column chromatography (CC) using as stationary phases silica gel and Sephadex LH-20. As shown, the purity of monomeric alkannin/shikonin was greater by HSCCC than CC separation of commercial A/S samples.

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Section: Introductionmentioning

confidence: 99%

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high‐speed counter‐current chromatography

Assimopoulou

Sturm

Stuppner

et al. 2008

Biomedical Chromatography

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“…Compounds 1, 2 and 3 were identified as physcion [12], chrysophanol [13] and 10,10′-chrysophanol bianthrone [14] respectively by comparing their TLC chromatograms, EI-MS, 1 H NMR and 13 C NMR spectra with authentic samples and published data. The fragment ions at m/z 270 and 269 correspond to physcion or isophyscion-9-anthrone and those at 241 and 240 belong to chrysophanol-9-anthrone.…”

Section: Resultsmentioning

confidence: 99%

Chemical Constituents and Insecticidal Activity of <i>Senna italica</i> Mill. from the Sudan

Yagi

Tigani

Ali

et al. 2013

ILCPA

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The chemical constituents and insecticidal activity of the pods of Senna italica (Caesalpiniaceae, Fabaceae) were investigated. From the chloroform extract of pods, physcion, chrysophanol, chrysophanol-10,10′-bianthrone, chrysophanol-physcion bianthrone and chrysophanolisophyscion bianthrone, were isolated and characterized using different spectral methods. The GC-MS of the n-hexane extract revealed that the major chemical constituents of the oil were 2,6,-di-secbutylphenol (36.69 %), di-n-octylphthalate (12.06 %), eicosane (5.46 %), tetratriacontane (4.87 %) and 2,2'-methylenebis[6-(1,1-dimethyl)-4-methylphenol (4.18 %). The n-hexane extract showed potent insecticidal activity (100 %) against Callosbruchus analis F. This study suggests that S. italica pods cannot be a source of sennosides but for other bioactive compounds.

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“…1C). These compounds were isolated by preparative TLC and identified by 1 H NMR by comparison with data in the literature as chrysophanol (R f , 0.72), physcion (R f , 0.7), aloe emodin (R f , 0.24) (Liu et al, 2004), and emodin (R f , 0.37) (Meselhy, 2003;Liu et al, 2004;Shun et al, 2006). MIC values were determined for the CE1, CE2, and the four isolated constituents.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of the IraqiRheum ribes. Root

Alaadin

Al-Khateeb

Jäger

2007

Pharmaceutical Biology

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The antibacterial activity of the ethanol, aqueous, and organic extracts from the root of Rheum ribes Linn (Polygonaceae) was examined. Four anthraquinone aglycone components, chrysopahnol, physcion, aloe emodin, and emodin, were isolated from the biologically active extract and identified by spectroscopic analysis. Emodin is recorded for the first time in this species. The MIC values of the biologically active extracts, aloe emodin, and emodin, were 500, 125, 250, and 63 mg=mL against Staphylococcus aureus, respectively. The extracts and compounds did not inhibit Pseudomonas aeruginosa and Escherichia coli at the highest concentration tested, 4000 and 250 mg=mL, respectively.

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Preparative isolation and purification of hydroxyanthraquinones and cinnamic acid from the Chinese medicinal herb Rheum officinale Baill. by high-speed counter-current chromatography

Cited by 103 publications

References 11 publications

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high‐speed counter‐current chromatography

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high‐speed counter‐current chromatography

Chemical Constituents and Insecticidal Activity of <i>Senna italica</i> Mill. from the Sudan

Antibacterial Activity of the IraqiRheum ribes. Root

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