Preparative separation and purification of rosmarinic acid from perilla seed meal via combined column chromatography

Tang, Wei-Zhuo; Sun, Baoshan; Zhao, Yanfang

doi:10.1016/j.jchromb.2013.12.007

Cited by 32 publications

(23 citation statements)

References 24 publications

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“…Natural plant seeds are the major source of phytonutrients, including phenolic compounds and flavonoids. A large number of reports revealed perilla seed was chosen as a beneficial functional food due to its abundance of phenolic acids including caffeic acid, rosmarinic acid, and their glucosides as well as flavonoids, such as apigenin and luteolin (Ha et al, ; Lee et al, ; Peng, Ye, & Kong, ; Tang et al, ; Zhou et al, ). Rosmarinic acid, apigenin, and luteolin are plant secondary metabolites that are well known for their hydrophilic anti‐oxidative and anti‐inflammatory properties (Asif, ; Lee et al, ; Zhou et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…The declining expression of COX-2 by PSME was also presented in the same direction (Figure 8b). and their glucosides as well as flavonoids, such as apigenin and luteolin (Ha et al, 2012;Lee et al, 2013;Peng, Ye, & Kong, 2005;Tang et al, 2014;Zhou et al, 2014). Rosmarinic acid, apigenin, and luteolin are plant secondary metabolites that are well known for their hydrophilic anti-oxidative and anti-inflammatory properties (Asif, 2012;Lee et al, 2013;Zhou et al, 2014).…”

Section: Effect Of Pse and Psme On Inos And Cox-2 Mrna Expressionmentioning

confidence: 99%

“…This by‐product is normally discarded or used in animal feeds as a low‐value material. Although, some studies have pointed out the perilla seed meal still contained high‐quality digestible proteins (Longvah & Deosthale, ), polysaccharides, and substantial amounts of phenolic antioxidants (Tang, Sun, & Zhao, ; Zhou et al, ). The most abundant of phenolic compounds in perilla seed meal by‐product are rosmarinic acid, rosmarinic acid‐3‐O‐β‐D‐glycoside, caffeic acid, and caffeic acid‐3‐O‐β‐D‐glycoside (Zhou et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…, polysaccharides, and substantial amounts of phenolic antioxidants (Tang, Sun, & Zhao, 2014;Zhou et al, 2014).…”

mentioning

confidence: 99%

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Potential anti-mutagenicity, antioxidant, and anti-inflammatory capacities of the extract from perilla seed meal

et al. 2018

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The objective of this study was to investigate the in vitro bioactivities associated with the content of phytochemicals in the extracts from perilla seed meal extract (PSME) compared with dietary seed (PSE). PSE had higher total phenolics and flavonoids content than PSME. However, hydrophilic phytochemical contents in PSME were quantitatively equivalent to PSE. Rosmarinic acid was predominantly found in both extracts. Cell viability and anti‐mutagenicity testing demonstrated that PSE and PSME were biosecured and non‐genotoxic. Both extracts strongly scavenged free radicals and significantly reduced reactive oxygen species (ROS) production. The extracts drastically diminished nitric oxide (NO) production of LPS‐treated RAW 264.7 cells via iNOS mRNA expression. The expression of IL‐6 and COX‐2 were evidently inhibited by these extracts. It could be concluded that PSE and PSME clearly showed in vitro anti‐mutagenicity, antioxidant and anti‐inflammatory capacities. In particular, the by‐product perilla seed meal could be considered as a high nutritive functional food. Practical applications This study suggests that the seed meals, a by‐product from seed oil industry, can be utilized as a valuable dietary source for humans and animals. The high content of polyphenols and their bioactivities can be developed as functional foods, and excipients and fillers in pharmaceuticals and nutraceuticals production. Moreover, recycling of the by‐product seed meals should also reduce environmental and sanitary pollution.

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Section: Discussionmentioning

confidence: 99%

Section: Effect Of Pse and Psme On Inos And Cox-2 Mrna Expressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…, polysaccharides, and substantial amounts of phenolic antioxidants (Tang, Sun, & Zhao, 2014;Zhou et al, 2014).…”

mentioning

confidence: 99%

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Potential anti-mutagenicity, antioxidant, and anti-inflammatory capacities of the extract from perilla seed meal

et al. 2018

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“…As well as protecting the human body from diseases such as cancer, inflammation, rheumatoid arthritis and cardiovascular disorders (Tang et al, 2014), antioxidants are also able to prevent food spoilage. Recently, interest in the antioxidant properties of plant seed oils and their benefits to human health has increased considerably.…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties, fatty acid composition and antioxidant activity

Zhang

et al. 2017

Food Sci. Technol

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Response surface methodology (RSM) was used to optimize ultrasound-assisted aqueous enzymatic extraction (UAAEE) conditions for perilla seed oil. Under the optimum conditions-which were a liquid-to-solid ratio of 4.4:1, a hydrolysis time of 2.66 h, a hydrolysis temperature of 50.87 °C and an ultrasound treatment time of 24.74 min-an oil yield of 31.34% was obtained. Comparisons of the physicochemical characteristics of oil obtained using UAAEE with those of oil obtained by solvent extraction (SE oil) and cold pressing extraction (CPE oil) revealed similar refractive indices and saponification values, but UAAEE oil had a higher iodine value and better stability against oxidation, with a low peroxide value. UAAEE oil contained higher levels of beneficial α-linolenic acid and phenolics than SE oil or CPE oil, and it also had superior efficiency in scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals.Keywords: perilla seed oil; ultrasound-assisted aqueous enzymatic extraction; physical and chemical characteristics; fatty acid composition; antioxidant activities.Practical Application: Based on its physical and chemical properties, perilla oil obtained using UAAEE would be suitable as high quality edible oil and could also have applications in the cosmetic and pharmaceutical industries.

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Orthogonal separation protocol for the simultaneous preparation of four medically active compounds from Anemarrhenae Rhizoma by sequential polyamide and macroporous resin adsorbent chromatography

Nian

Liu

Fan

et al. 2016

J of Separation Science

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The green and efficient preparation of natural products from biomass is considered an important area of interest in pharmaceutical applications. In this study, we aimed to provide a practical example with a popular traditional Chinese medicine, Anemarrhenae Rhizome, and showcase the orthogonal use of column chromatography with polyamide and macroporous adsorption resins to selectively concentrate and efficiently purify four bioactive compounds: neomangiferin (NMF), mangiferin (MF), timosaponin B-II (TS B-II), and timosaponin A-III (TS A-III). First, polyamide T60-100 was employed to fractionalize the crude extracts of Anemarrhenae Rhizome. Macroporous resin HPD400 was subsequently used to purify the xanthones and steroidal saponins. Under the optimized conditions, 2.31 g of NMF, 4.10 g of MF, 12.87 g of TS B-II, and 2.78 g of TS A-III were prepared from 1 kg of crude materials, and their purities were 90.0, 92.15, 90.8, and 92.61%, respectively. The results of this study indicate that the combined column chromatography with polyamide and macroporous adsorption resins can be referenced as a green and efficient alternative for large-scale purification of bioactive ingredients from herbal raw materials.

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Preparative separation and purification of rosmarinic acid from perilla seed meal via combined column chromatography

Cited by 32 publications

References 24 publications

Potential anti-mutagenicity, antioxidant, and anti-inflammatory capacities of the extract from perilla seed meal

Potential anti-mutagenicity, antioxidant, and anti-inflammatory capacities of the extract from perilla seed meal

Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties, fatty acid composition and antioxidant activity

Orthogonal separation protocol for the simultaneous preparation of four medically active compounds from Anemarrhenae Rhizoma by sequential polyamide and macroporous resin adsorbent chromatography

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