Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle‐pressure chromatogram isolated gel column coupled with two‐dimensional liquid chromatography

Abstract

We established a two-dimensional strong cation exchange/reversed-phase liquid chromatography protocol to isolate and purify isoquinoline alkaloids from Corydalis impatiens. Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a medium-pressure chromatographic tower containing middle chromatogram isolated gel. A strong cation exchange column was employed to separate and obtain 30 fractions. We chose fractions 22-29 for reversed-phase liquid chromatography purification using characteristic isoquinoline alkaloid ultraviolet absorption spectra. Several isoquinoline alkaloid fractions (22-29) were further separated, and those of low resolution were isolated via two-dimensional liquid chromatography in the orthogonal plane. A total of eight novel isoquinoline alkaloids with characteristic ultraviolet spectra were obtained from C. impatiens. We thus demonstrate the benefits of off-line two-dimensional strong cation exchange/reversed-phase liquid chromatography to isolate isoquinoline alkaloids from C. impatiens.

Research on the Mechanism of Wuwei Yuganzi San in the Treatment of Coronary Heart Disease Based on Network Pharmacology

“…Retrieving Results of Effective Ingredients of WYS 92 ingredients for YGZ, 92 ingredients for DH, and 148 ingredients for GJ were obtained from TCMSP online platform. 31 ingredients for SBZJ and 33 ingredients for ZJJE were acquired from the literature [18][19][20][21][22]. When ADME was set as OB≥ 30% and DL ≥ 0.18 in TCMSP online platform, the probability ≥ 0.88 in Swiss Target Prediction online platform, and the norm t ≥ 0.88 in PharmMapper online platform, 13 ingredients and 370 targets for YGZ, 12 ingredients, and 353 targets for DH, 1 ingredient and 1 target for GJ, 1 ingredient and 2 targets for SBZJ, 9 ingredients and 34 targets for ZJJR.…”

Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography

“…A frequently employed procedure for enriching bioactives from a variety of NPs is NPs MCI GEL® CHP20P MPLC, bearing the advantages of excellent reproducibility, on-line detection, and automatic control and was selected for pretreating our samples. [39][40][41] As far as we are aware, there were no report on the chromatographic recognition, isolation and purication DPPH inhibitors from NPs except our laboratory reports. In our earlier work, we carried out the targeted separation of DPPH inhibitors originating from Saxifraga atrata by making use of three-steps of MPLC, RPLC, and HILIC regulated using an on-line HPLC-DPPH screening to preparative isolation of the DPPH inhibitors (11-O-galloylbergenin, ethyl gallate, isoquercitrin, and rutin).…”

Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro

“…As such, two-dimensional HPLC (2D HPLC) has emerged as a more practical approach to separating individual compounds from complex natural products, enabling marked improvements in both separation selectivity and peak capacity. [25][26][27] Owing to its reproducibility, universality, and excellent capacity, most HPLC separation approaches rely on reversed-phase liquid chromatography (RPLC) approach, which is very classical. 28,29 However, due to similar separation principles, RPLC/RPLC exhibits low orthogonality.…”