Preparative separation of specific triterpenoids from Inonotus obliquus based on negative-pressure cavitation extraction and high-speed counter-current chromatography

Kou, Ping; Wang, Sui-Xin; Pan, Hongyi; Wan, Ning; Wang, Xiqing; Liu, Zhiguo; Zhao, Chunjian; Jiang, Shougang; Fu, Yujie

doi:10.1016/j.jtice.2021.06.024

Cited by 7 publications

(2 citation statements)

References 19 publications

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“…[24][25][26] Compared to conventional liquid-solid separation methods, HSCCC is a unique liquid-liquid partition chromatography method in which a solid support is not used. 27,28 This strategy eliminates the irreversible adsorptive loss of the samples onto the solid support matrix used in a conventional chromatographic column. 29 Semi-preparative HPLC techniques have also been used for the isolation of active ingredients from natural medicines and have been found to be a high-efficiency and low-cost approach for separating bioactive compounds from crude extracts of G. lucidum, illustrating their potential for industrial applications.…”

Section: Introductionmentioning

confidence: 99%

“…HSCCC and semi‐preparative HPLC are widely used in the modern separation field due to their advantages of high automation and high compound purity 24–26 . Compared to conventional liquid–solid separation methods, HSCCC is a unique liquid–liquid partition chromatography method in which a solid support is not used 27,28 . This strategy eliminates the irreversible adsorptive loss of the samples onto the solid support matrix used in a conventional chromatographic column 29 .…”

Section: Introductionmentioning

confidence: 99%

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Efficient and fast screening and separation based on computer‐aided screening and complex chromatography methods for lipoxygenase inhibitors from Ganoderma lucidum

Nong,

Zhou,

et al. 2024

Phytochemical Analysis

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IntroductionAccurate screening and targeted preparative isolation of active substances from natural medicines have long been technical challenges in natural medicine research.ObjectivesThis study outlines a new approach for improving the efficiency of natural product preparation, focusing on the rapid and accurate screening of potential active ingredients in Ganoderma lucidum and efficient preparation of lipoxidase inhibitors, with the aim of providing new ideas for the treatment of Alzheimer's disease with G. lucidum.MethodsThe medicinal plant G. lucidum was selected through ultrafiltration coupled with liquid chromatography and mass spectrometry (UF–LC–MS) and computer‐assisted screening for lipoxygenase (LOX) inhibitors. In addition, the inhibitory effect of the active compounds on LOX was studied using enzymatic reaction kinetics, and the underlying mechanism is discussed. Finally, based on the earlier activity screening guidelines, the identified ligands were isolated and purified through complex chromatography (high‐speed countercurrent chromatography and semi‐preparative high‐performance liquid chromatography).ResultsFive active ingredients, ganoderic acids A, B, C2, D2, and F, were identified and isolated from G. lucidum. We improved the efficiency and purity of active compound preparation using virtual computer screening and enzyme inhibition assays combined with complex chromatography.ConclusionThe innovative methods of UF–LC–MS, computer‐aided screening, and complex chromatography provide powerful tools for screening and separating LOX inhibitors from complex matrices and provide a favourable platform for the large‐scale production of bioactive substances and nutrients.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient and fast screening and separation based on computer‐aided screening and complex chromatography methods for lipoxygenase inhibitors from Ganoderma lucidum

Nong,

Zhou,

et al. 2024

Phytochemical Analysis

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Procedures to evaluate potential of plants as natural food preservatives: Phytochemical characterization, novel extraction technology, and safety evaluation—A comprehensive review

Ahmadi,

Mosleh,

Yeganeh

et al. 2024

Food Science & Nutrition

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There is increasing demand for natural food preservative in food manufacturing industry as it is the key to meet consumers' preferences toward healthier food choice. Plant is listed among the most important resources of bioactive components to be utilized as the green and natural food preservatives. There are more than 10,000 kinds of bioactive components in plants that possess antioxidant and antimicrobial properties. Artemisia with potential antimicrobial and antioxidant attributes, as well as functional and medicinal properties, is one of the most important plant species. The manuscript presents a comprehensive review of the potential of the Artemisia species as natural food preservatives. The current challenges and ways forward in using Artemisia EOs and extracts as food preservatives are also discussed. This topic is timely and important considering the natural preservatives used to replace chemical ingredients, sustaining quality, healthy properties, and shelf life of food products as well as efficient and novel extraction techniques.

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Essential oil extraction from lavender using negative pressure cavitation extraction and coating to extend grape shelf life

Gul,

Dar,

Dash

et al. 2023

Applied Food Research

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Preparative separation of specific triterpenoids from Inonotus obliquus based on negative-pressure cavitation extraction and high-speed counter-current chromatography

Cited by 7 publications

References 19 publications

Efficient and fast screening and separation based on computer‐aided screening and complex chromatography methods for lipoxygenase inhibitors from Ganoderma lucidum

Efficient and fast screening and separation based on computer‐aided screening and complex chromatography methods for lipoxygenase inhibitors from Ganoderma lucidum

Procedures to evaluate potential of plants as natural food preservatives: Phytochemical characterization, novel extraction technology, and safety evaluation—A comprehensive review

Essential oil extraction from lavender using negative pressure cavitation extraction and coating to extend grape shelf life

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