Preparing 3D-printable silk fibroin hydrogels with robustness by a two-step crosslinking method

Graphic abstract Nowadays, awareness about the burn wound is often considered difficult due to bacterial and other organism infections. The facile and eco-friendly preparations of antibiotic-loaded hydrogel-based bio-composites have great attention in the field of wound dressing for burn wound therapy and nursing care. In the present investigation, we have developed ciprofloxacin (CF)-encapsulated graphene-silk fibroin macromolecular hydrogel dressings material with unique chemical and physical properties to achieve the desirable antibacterial efficacy and healing activity. The antibacterial activity of prepared hydrogel was evaluated against bacterial pathogens treated with different concentrations of CF, which have been provided improved antibacterial activity on burn wound infection. In vitro , cytocompatibility evaluations were performed to imply the suitability of hydrogel on fibroblast cells, which has been dramatically related to in vivo wound healing. Furthermore, an in vivo wound healing analysis was carried out using a rat to observe the capability of the CF-incorporated GH/SF hydrogel matrix. Thus, this investigation widely demonstrates the healing ability of prepared hydrogel matrix and could be a significant landmark in the research on burn wound healing applications.

show abstract

“…The supernatant was stored at 4 °C. Finally, the concentration of SF solution was measured at the weighing method [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Facile design and development of nano-clustery graphene-based macromolecular protein hydrogel loaded with ciprofloxacin to antibacterial improvement for the treatment of burn wound injury

Zhu

Chen

2021

Polym. Bull.

View full text Add to dashboard Cite

show abstract

“…In one study, Gong et al utilized a two-step method for formulating silk fibroin hydrogel as a new kind of bioink with outstanding mechanical properties and biocompatibility for tissue engineering [ 84 ]. A double-network hydrogel with acceptable shear-thinning behaviors, great strength, and good resilience for 3D printing was synthesized.…”

Section: Natural Polymers As Bioink For Corneal Tissue Engineeringmentioning

confidence: 99%

3D Printed Hydrogels for Ocular Wound Healing

et al. 2022

View full text Add to dashboard Cite

Corneal disease is one of the most significant causes of blindness around the world. Presently, corneal transplantation is the only way to treat cornea blindness. It should be noted that the amount of cornea that people donate is so much less than that required (1:70). Therefore, scientists have tried to resolve this problem with tissue engineering and regenerative medicine. Fabricating cornea with traditional methods is difficult due to their unique properties, such as transparency and geometry. Bioprinting is a technology based on additive manufacturing that can use different biomaterials as bioink for tissue engineering, and the emergence of 3D bioprinting presents a clear possibility to overcome this problem. This new technology requires special materials for printing scaffolds with acceptable biocompatibility. Hydrogels have received significant attention in the past 50 years, and they have been distinguished from other materials because of their unique and outstanding properties. Therefore, hydrogels could be a good bioink for the bioprinting of different scaffolds for corneal tissue engineering. In this review, we discuss the use of different types of hydrogel for bioink for corneal tissue engineering and various methods that have been used for bioprinting. Furthermore, the properties of hydrogels and different types of hydrogels are described.

show abstract

“…In addition, the rheological behaviour of bioinks plays pivotal role in determination of printing quality and consistency in extrusionbased bioprinting process. 6,19,34 Therefore, monitoring of rheological material functions is important for determination of hydrogel-based formulations properties and development of bioinks with highprinting performance. Bioinks that exhibit viscous shear-thinning and appropriate range of loss modulus (G 00 ) to storage modulus (G 0 ) ratio (loss tangent, G 00 /G 0 ) would be considered as preferred bioink that provide self-support during material deposition process while ensuring uniform extrusion process without any needle clogging.…”

Section: Immunohistochemical and Histological Evaluation Of Constructsmentioning

confidence: 99%

3D bioprinting of photo‐crosslinkable silk methacrylate (SilMA)‐polyethylene glycol diacrylate (PEGDA) bioink for cartilage tissue engineering

Bandyopadhyay

Mandal

Bhardwaj

2021

J Biomedical Materials Res

View full text Add to dashboard Cite

Articular cartilage damage poses huge burden on healthcare sector globally due to its extremely weak inherent regenerative ability. Three-dimensional (3D) bioprinting for development of cartilage mimic constructs using composite bioinks serves as an emerging perspective. However, difficulty in development of suitable bioink and chemical crosslinking associated inherent toxicity hamper widespread adoption of this technique. To circumvent this, a photo-polymerizable hydrogel-based bioink

show abstract

Preparing 3D-printable silk fibroin hydrogels with robustness by a two-step crosslinking method

Abstract: Schematic showing the fabrication process of the 3D-printed robust double-network RSF hydrogels.

Cited by 22 publications

References 33 publications

Facile design and development of nano-clustery graphene-based macromolecular protein hydrogel loaded with ciprofloxacin to antibacterial improvement for the treatment of burn wound injury

Facile design and development of nano-clustery graphene-based macromolecular protein hydrogel loaded with ciprofloxacin to antibacterial improvement for the treatment of burn wound injury

3D Printed Hydrogels for Ocular Wound Healing

3D bioprinting of photo‐crosslinkable silk methacrylate (SilMA)‐polyethylene glycol diacrylate (PEGDA) bioink for cartilage tissue engineering

Contact Info

Product

Resources

About