Preparing Platelet-Rich Plasma with Whole Blood Harvested Intraoperatively During Spinal Fusion

Abstract

BackgroundPlatelet-rich plasma (PRP) has gained growing popularity in use in spinal fusion procedures in the last decade. Substantial intraoperative blood loss is frequently accompanied with spinal fusion, and it is unknown whether blood harvested intraoperatively qualifies for PRP preparation.Material/MethodsWhole blood was harvested intraoperatively and venous blood was collected by venipuncture. Then, we investigated the platelet concentrations in whole blood and PRP, the concentration of growth factors in PRP, and the effects of PRP on the proliferation and viability of human bone marrow-derived mesenchymal stem cells (HBMSCs).ResultsOur results revealed that intraoperatively harvested whole blood and whole blood collected by venipuncture were similar in platelet concentration. In addition, PRP formulations prepared from both kinds of whole blood were similar in concentration of platelet and growth factors. Additional analysis showed that the similar concentrations of growth factors resulted from the similar platelet concentrations of whole blood and PRP between the two groups. Moreover, these two kinds of PRP formulations had similar effects on promoting cell proliferation and enhancing cell viability.ConclusionsTherefore, intraoperatively harvested whole blood may be a potential option for preparing PRP spinal fusion.

Risk Factors for Poor Prognosis of Cervical Spinal Cord Injury with Subaxial Cervical Spine Fracture-Dislocation After Surgical Treatment: A CONSORT Study

“…The results of multivariate analysis showed that SLIC score (more than 7.5) could be a risk factor for poor prognosis of cervical SCI with subaxial cervical fracture-dislocation after surgical treatment, because the degree of the cervical SCI aggravates as the SLIC score increases, which indicates poor fracture morphology, severe cervical nerve injury, or SLC complex injury. Full functional recovery of the cervical spinal cord is hard to achieve for patients with a degree of spinal cord compression more than 50% in the acute injury mode [ 27 – 29 ]. Our study showed that MSCC of more than 55.8% can result in poor prognosis of cervical SCI with inferior cervical fracture-dislocation after surgical treatment, and our results are consistent with previous studies and could also be helpful for the spinal surgeon in deciding on the optimal operation time.…”