PRESCIENT: platform for the rapid evaluation of antibody success using integrated microfluidics enabled technology

Wippold, Jose A; Wang, Han; Tingling, Joseph D.; Leibowitz, Julian L.; Figueiredo, Paul de; Han, Arum

doi:10.1039/c9lc01165j

Cited by 25 publications

(33 citation statements)

References 46 publications

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“…The coronavirus used in this study is mouse hepatitis virus strain A59 (MHV‐A59) and has been described previously (Bond et al, 1979; Wippold et al, 2020). Mouse L2 cells, which are susceptible to MHV infection, were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 4 mM glutamine and 10% defined calf serum (Hyclone) at 37°C 5% CO 2 environment (Sturman & Takemoto, 1972).…”

Section: Methodsmentioning

confidence: 99%

Sub‐second heat inactivation of coronavirus using a betacoronavirus model

Jiang

Zhang

Wippold

et al. 2021

Biotech & Bioengineering

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Heat treatment denatures viral proteins that comprise the virion, making the virus incapable of infecting a host. Coronavirus (CoV) virions contain single‐stranded RNA genomes with a lipid envelope and four proteins, three of which are associated with the lipid envelope and thus are thought to be easily denatured by heat or surfactant‐type chemicals. Prior studies have shown that a temperature as low as 75°C with a treatment duration of 15 min can effectively inactivate CoV. The degree of CoV heat inactivation greatly depends on the length of heat treatment time and the temperature applied. With the goal of finding whether sub‐second heat exposure of CoV can sufficiently inactivate CoV, we designed and developed a simple fluidic system that can measure sub‐second heat inactivation of CoV. The system is composed of a stainless‐steel capillary immersed in a temperature‐controlled oil bath followed by an ice bath, through which virus solution can flow at various speeds. Flowing virus solution at different speeds, along with temperature control and monitoring system, allows the virus to be exposed to the desired temperature and treatment durations with high accuracy. Using mouse hepatitis virus, a betacoronavirus, as a model CoV system, we identified that 71.8°C for 0.51 s exposure is sufficient to obtain >5 Log 10 reduction in viral titer (starting titer: 5 × 10 7 PFU/ml), and that when exposed to 83.4°C for 1.03 s, the virus was completely inactivated (>6 Log 10 reduction).

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Section: Methodsmentioning

confidence: 99%

Sub‐second heat inactivation of coronavirus using a betacoronavirus model

Jiang

Zhang

Wippold

et al. 2021

Biotech & Bioengineering

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“…In this platform, a single hybridoma cell was encapsulated in the droplet produces neutralizing antibodies against murine hepatitis virus. The merged droplet of the hybridoma cell and the virus was then merged with the host cell droplet and the hybridoma cells were screened based on the prototype fluorescence imaging of the infection of the host by the virus (Wippold et al, 2020) (Figure 7B). These microfluidic systems are promising tools to generate cell pairs for studying cell-cell interactions or screening hybridoma cells against surface receptors.…”

Section: Sorting Of Hybridoma Cellsmentioning

confidence: 99%

Application of Microfluidic Technology in Field of Antibody Preparation

Sun¹,

Hu²,

wang³

2021

Preprint

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Microfluidic technology is a science and technology that can accurately manipulate fluids in micro-sized channels. In recent years, microfluidic devices have attracted wide attention due to its easy manipulation, miniaturized size, high throughput and precise control, which provide a potential platform for antibody screening. This review paper provides an overview of recent advances in microfluidic methods application in the field of antibody preparation. While hybridoma technology and four antibody engineering techniques including phage display, single B cell antibody screening, antibody expression and cell-free protein synthesis are mainly introduced, important advances of experimental models and results are also discussed. Furthermore, the authors expound on the limitations of current microfluidic screening system and present future directions of antibody screening platform based on microfluidics. Antibody preparation on microfluidics combined with other technologies has huge application potential in the field of biomedicine, and it is anticipated to be further developed.

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“…It is a common practice to use fluorescent elements to indicate the detection of a target. These elements can be added to the device [ 63 , 89 , 93 ], attached to the target element [ 84 , 90 , 132 ] or replicated during amplification steps [ 5 , 101 , 133 ]. Examples of fluorescent elements used in microfluidics are cyanine-5 (Cy5), methylene blue (MB) [ 79 ], fluorescein [ 77 ], fluorescein amidites (FAM), hexachlorinated fluorescein (HEX) [ 110 ], SYTO-82 [ 134 ], AF 488 [ 56 ], EvaGreen and SYBR Green [ 81 , 107 ].…”

Section: User Interface: Signs Of Detection In Microfluidic Systemsmentioning

confidence: 99%

“…Fluorescence is a very versatile method that can be used in different materials (e.g. paper [ 41 ], polymers [ 63 ] and structure-free [ 89 ]). It can also be adapted to automated analysis via smartphones or desktop scanners [ 107 , 135 ].…”

Section: User Interface: Signs Of Detection In Microfluidic Systemsmentioning

confidence: 99%

Microfluidic devices for the detection of viruses: aspects of emergency fabrication during the COVID-19 pandemic and other outbreaks

2020

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Extensive testing of populations against COVID-19 has been suggested as a game-changer quest to control the spread of this contagious disease and to avoid further disruption in our social, healthcare and economical systems. Nonetheless, testing millions of people for a new virus brings about quite a few challenges. The development of effective tests for the new coronavirus has become a worldwide task that relies on recent discoveries and lessons learned from past outbreaks. In this work, we review the most recent publications on microfluidics devices for the detection of viruses. The topics of discussion include different detection approaches, methods of signalling and fabrication techniques. Besides the miniaturization of traditional benchtop detection assays, approaches such as electrochemical analyses, field-effect transistors and resistive pulse sensors are considered. For emergency fabrication of quick test kits, the local capabilities must be evaluated, and the joint work of universities, industries, and governments seems to be an unequivocal necessity.

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PRESCIENT: platform for the rapid evaluation of antibody success using integrated microfluidics enabled technology

Abstract: Identifying antibodies (Abs) that neutralize infectious agents is the first step for developing therapeutics, vaccines, and diagnostic tools for these infectious agents.

Cited by 25 publications

References 46 publications

Sub‐second heat inactivation of coronavirus using a betacoronavirus model

Sub‐second heat inactivation of coronavirus using a betacoronavirus model

Application of Microfluidic Technology in Field of Antibody Preparation

Microfluidic devices for the detection of viruses: aspects of emergency fabrication during the COVID-19 pandemic and other outbreaks

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