Presence and characterization of Mycobacterium avium subspecies paratuberculosis from clinical and suspected cases of Crohn's disease and in the healthy human population in India

Abstract

Paper documented the presence of MAP in all patients with Crohn's disease, in some animal attendants who had the history of working with goat herds infected with Johne's disease and in few normal healthy individuals. Presence of Ziehl Neelsen positive MAP. In the stool of attendants working with MAP-infected animals was unique to humans. ELISA based on antigens derived from indigenous MAP 'bison type' genotype of goat origin was most sensitive modality for screening Crohn's disease patients.

Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

“…Indigenous g-ELISA was superior than AGPT for the diagnosis of MAP infection in cattle (Pahangchopi et al 2014). In another study, sensitivity and specificity of indigenous ELISA kit was 66.6 and 75.0% and 68.1 and 66.6% with tissue culture and tissue PCR, respectively (Singh et al, 2008). For estimating sensitivity and specificity of ELISA in case of MAP infection is by comparing serum ELISA with fecal culture (Garg et al, 2015).…”

Evalution of “indirect fluorescent antibody test‟ as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock

“…Udder of each lactating animal was washed and cleaned before milk sample (15 ml Singh et al (2008) and examined under oil under immersion (×100) for pink coloured acid-fast bacilli (AFB) indistinguishable to MAP (Singh et al, 2008).…”

“…Crohn"s Disease (CD), a systemic disorder in humans where principal clinico-pathological manifestation is the chronic inflammation of the intestines. Increasing evidences suggest that Crohn's disease has an infectious etiology and MAP being the most plausible candidate since it has been frequently reported from commercial milk and milk products globally (Grant et al, 2002;Ikonomopoulos et al, 2005;Ellingson et al, 2005;Ayele et al, 2005;Paolicchi et al, 2012) and has also been isolated from patients suffering from CD (Singh et al, 2008;Naser et al, 2014).…”

Recent Approaches in Diagnosis and Control of Mycobacterial Infections with Special Reference to Mycobacterium Avium Subspecies

“…In India disease has been reported from all the domestic ruminants [cattle, buffaloes, goats, sheep (Singh et al, 2010a), wild ruminants [blue bulls, hog deer, bison (Singh et al, 2010b, Singh et al, 2011a, other animals [rabbit (Singh et al, 2012b)], primates (Singh et al, 2011b), human beings (Singh et al, 2008), environment [soil and water resources (Singh et al, 2012a)], milk [raw milk, pasteurized milk and milk products (Shankar et al, 2010). …”