Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains

Szalo, Ioan Mihai; Goffaux, Frédéric; Pirson, Vinciane; Piérard, Denis; Ball, H.J.; Mainil, Jacques

doi:10.1016/s0923-2508(02)01379-7

Cited by 69 publications

(51 citation statements)

References 28 publications

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“…Previously, it was demonstrated that the lpfA1 and lpfA2 genes are highly prevalent among LEE-positive E. coli strains, including EHEC O157:H7 strains associated with severe and/or epidemic disease (28,31,34). Further, homologues of lpf genes have also been detected in non-O157:H7 LEE-positive E. coli strains, LEE-negative pathogenic E. coli strains, and REPEC strains (8,21,23,32).…”

Section: Resultsmentioning

confidence: 99%

“…Because our data indicated that LPF constitute an important colonization factor of EHEC O157:H7 strains and because cumulative evidence indicates that homologues to the lpf genes are found in other pathogenic E. coli and Salmonella strains (7,8,28,31,34), in the current study, we identified several polymorphisms within the lpfA genes, which were used to classify the major fimbrial subunit genes into distinct variants. Further, we showed that lpf genes, in combination with the different intimin types, are reliable markers for the differentiation of E. coli O157:H7 and other pathogenic E. coli strains.…”

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confidence: 93%

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Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates

et al. 2009

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Lpf (stands for long polar fimbriae) is one of the few adhesive factors of enterohemorrhagic Escherichia coli O157:H7 associated with colonization of the intestine. E. coli O157:H7 strains possess two lpf loci encoding highly regulated fimbrial structures. Database analysis of the genes encoding the major fimbrial subunits demonstrated that they are present in commensal as well as pathogenic (both intestinal and extraintestinal) E. coli strains and in Salmonella strains and that the lpfA1 and lpfA2 genes are highly prevalent among LEE (locus of enterocyte effacement)-positive E. coli strains associated with severe and/or epidemic disease. Further DNA sequence analysis of the lpfA1 and lpfA2 genes from different attaching-and-effacing E. coli strains has led us to the identification of several polymorphisms and the classification of the major fimbrial subunits into distinct variants. Using collections of pathogenic E. coli isolates from Europe and Latin America, we demonstrated that the different lpfA types are associated with the presence of specific intimin (eae) adhesin variants and, most importantly, that they are found in specific E. coli pathotypes. Our results showed that the use of these fimbrial genes as markers, in combination with the different intimin types, resulted in a specific test for the identification of E. coli O157:H7, distinguishing it from other pathogenic E. coli strains.

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Section: Resultsmentioning

confidence: 99%

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confidence: 93%

Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates

et al. 2009

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“…The bovine host specificity shown by O26 EHEC and enteropathogenic E. coli (EPEC) strains could be based on the production of a colonization factor, such as an adhesin, that is specific for cattle; such a colonization factor has been demonstrated to be the basis of the host specificity of classical human EPEC strains. Such an adhesin is likely to be a specific immunogen exposed at the bacterial cell surface (3,13,15,17,23,24).A monoclonal antibody (MAb), 2F3, was derived against a surface antigen extracted from the bovine O26 EHEC strain 4276; it tested positive in an enzyme-linked immunosorbent assay (ELISA) with all of the O26 and half of the O111 bovine and human EHEC and EPEC strains (9). Recent reexamination of six of the O111 EHEC and EPEC strains that tested positive revealed a contamination by O26 EHEC or EPEC strains.…”

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confidence: 99%

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confidence: 99%

2F3 Monoclonal Antibody Recognizes the O26 O-Antigen Moiety of the Lipopolysaccharide of EnterohemorrhagicEscherichia coliStrain 4276

Szalo

Taminiau

Goffaux

et al. 2004

Clin Vaccine Immunol

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Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and effacement. They are involved in enteric diseases both in humans and in animals, and EHEC strains can be responsible for hemolytic uremic syndrome in humans. Previously, it was shown that the 2F3 monoclonal antibody (MAb) is specific for the O26 EHEC and EPEC strains (P. Enterohemorrhagic Escherichia coli (EHEC) strains are an important class of pathogens for humans and animals. The term EHEC denotes E. coli strains that cause attaching and effacing lesions on epithelial cells, express Shiga toxin-encoding genes, possess a 60-MDa virulence plasmid, and produce hemorrhagic colitis and/or hemolytic uremic syndrome (17,18).KerrBesides those of the O157:H7 and O157:H Ϫ serotypes, EHEC strains of serogroup O26 are the most common strains found in human infections (4, 17). In addition, O26 EHEC strains are an important cause of enteritis in calves (12). One of the most important steps in the pathogenic mechanism of enteric bacteria is the initial adhesion of the bacteria to the intestinal wall. Several stages of colonization have been recognized in attaching and effacing E. coli pathogenesis, some of which await characterization. Some level of host specificity is shown by EHEC strains, but the role in host specificity of the recognized virulence factors, such as Shiga toxin 1 (Stx1) and/or Stx2 and the intimin protein (a protein that is encoded by the eae gene and necessary for the production of the attaching and effacing lesion), is unknown (8, 19). The bovine host specificity shown by O26 EHEC and enteropathogenic E. coli (EPEC) strains could be based on the production of a colonization factor, such as an adhesin, that is specific for cattle; such a colonization factor has been demonstrated to be the basis of the host specificity of classical human EPEC strains. Such an adhesin is likely to be a specific immunogen exposed at the bacterial cell surface (3,13,15,17,23,24).A monoclonal antibody (MAb), 2F3, was derived against a surface antigen extracted from the bovine O26 EHEC strain 4276; it tested positive in an enzyme-linked immunosorbent assay (ELISA) with all of the O26 and half of the O111 bovine and human EHEC and EPEC strains (9). Recent reexamination of six of the O111 EHEC and EPEC strains that tested positive revealed a contamination by O26 EHEC or EPEC strains. Purification of the strains in the mixed culture demonstrated that the O111 strains were 2F3 negative and the O26 strains were 2F3 positive (J. McCappin and H. Ball, unpublished data).Since the 2F3 MAb is specific for the O26 EHEC and EPEC strains, it may not only represent a diagnostic tool of these confirmed pathogens but also recognize a factor involved in the production of the attaching and effacing lesion. In view of these results, this study was initiated (i) to characterize the antigen recognized by the 2F3 MAb and its genetic determinant an...

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“…A introdução do operon EHEC lpf em E. coli K-12 resultou em um aumento da expressão de fímbrias e da adesão a células em cultivo (TORRES et al, 2002a). A subunidade principal de LP, lpfA, foi detectada em cepas de EHEC dos sorogrupos O157 e O145 (SZALO et al, 2002).…”

Section: Adesinas Fimbriais De Ehecunclassified

Escherichia coli produtora de toxina shiga (STEC): principais fatores de virulência e dados epidemiológicos

Bertão

Saridakis

2007

Semin. Cienc. Biol. Saude

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ResumoEscherichia coli produtora de toxina Shiga (STEC) é um importante patógeno veiculado por alimentos, principalmente produtos derivados de carne bovina e está associado a quadros de diarréias leves a severas e sanguinolentas. Em alguns indivíduos, a infecção por STEC pode progredir para a síndrome hemolítico-urêmica (HUS), seqüela caracterizada pela falência renal e a púrpura trombocitopênica trombótica (TTP), com possível envolvimento do sistema nervoso central. O gado bovino, geralmente saudável, é o principal reservatório de STEC, embora estas cepas também tenham sido isoladas de outros animais domésticos: ovelhas, cabras, cães, gatos e suínos. A principal característica de virulência, a produção de toxinas Shiga, não é suficiente para causar doenças e outros fatores são considerados relevantes, como a produção de enterohemolisina e de adesinas fimbriais e afimbriais. Embora as doenças humanas associadas a STEC sejam pouco descritas no Brasil, podemos observar uma significativa ocorrência destas cepas nos rebanhos bovinos, bem como a correlação entre sorotipos encontrados nestes animais e em pacientes humanos. Palavras-chave: Escherichia coli produtora de toxina Shiga (STEC). Diarréia. Bovinos. Fatores de virulência. AbstractShiga toxin producing Escherichia coli is an important food borne pathogen, mainly beef products, and is associated to mild and severe bloody diarrhea. In some individuals, STEC infection can progress to hemolytic-uremic syndrome (HUS), a sequela characterized by renal failure, and thrombotic thrombocytopenic purpura (TTP), with possible central nervous system involvement. Cattle, usually healthy, is the principal reservoir of STEC, although these strains have also been isolated from other domestic animals: sheep, goats, dogs, cats and pigs. The principal virulence feature, the production of Shiga toxins, is not enough to cause diseases, and other factors are considered important, as enterohemolysin and fimbrial and afimbrial adhesions production. Although human diseases associated to STEC have not been frequently reported in Brazil, their presence is frequent in cattle, as well as the correlation between serotypes found in these animals and human patients.

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Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains

Cited by 69 publications

References 28 publications

Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates

Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates

2F3 Monoclonal Antibody Recognizes the O26 O-Antigen Moiety of the Lipopolysaccharide of EnterohemorrhagicEscherichia coliStrain 4276

Escherichia coli produtora de toxina shiga (STEC): principais fatores de virulência e dados epidemiológicos

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