Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis

Gemski, P.; Lazere, Janet R.; Casey, Thomas A.; Wohlhieter, J. A.

doi:10.1128/iai.28.3.1044-1047.1980

Cited by 198 publications

(2 citation statements)

References 12 publications

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“…We used the food-borne clinical isolate Yptb -YPIII serotype O:3 88 , 89 as a model system for these studies. On the basis that conserved Cpx-signalling has been verified experimentally in other Yptb isolates 51 , 90 , and also in two other Yersinia species— Y. pestis 91 , 92 and Y. enterocolitica 79 , 93 , 94 , we assume that Cpx-signalling controls biofilm development in other Yersinia .…”

Section: Discussionmentioning

confidence: 99%

Cpx-signalling facilitates Hms-dependent biofilm formation by Yersinia pseudotuberculosis

Gahlot

Wai

Erickson

et al. 2022

npj Biofilms Microbiomes

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Bacteria often reside in sessile communities called biofilms, where they adhere to a variety of surfaces and exist as aggregates in a viscous polymeric matrix. Biofilms are resistant to antimicrobial treatments, and are a major contributor to the persistence and chronicity of many bacterial infections. Herein, we determined that the CpxA-CpxR two-component system influenced the ability of enteropathogenic Yersinia pseudotuberculosis to develop biofilms. Mutant bacteria that accumulated the active CpxR~P isoform failed to form biofilms on plastic or on the surface of the Caenorhabditis elegans nematode. A failure to form biofilms on the worm surface prompted their survival when grown on the lawns of Y. pseudotuberculosis. Exopolysaccharide production by the hms loci is the major driver of biofilms formed by Yersinia. We used a number of molecular genetic approaches to demonstrate that active CpxR~P binds directly to the promoter regulatory elements of the hms loci to activate the repressors of hms expression and to repress the activators of hms expression. Consequently, active Cpx-signalling culminated in a loss of exopolysaccharide production. Hence, the development of Y. pseudotuberculosis biofilms on multiple surfaces is controlled by the Cpx-signalling, and at least in part this occurs through repressive effects on the Hms-dependent exopolysaccharide production.

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Section: Discussionmentioning

confidence: 99%

Cpx-signalling facilitates Hms-dependent biofilm formation by Yersinia pseudotuberculosis

Gahlot

Wai

Erickson

et al. 2022

npj Biofilms Microbiomes

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“…Recently, Aleksic [15] reported the presence of Yersinia enterocolitica antigens 0 : 3, 0 : 8 and 0 : 9 in different Yersinia species and described their corresponding H antigens and origins. The fraction 1 envelope antigen (F1) of Yersinia pestis produced at 37 7C consists of a protein linked to polysaccharide (fraction 1A) which is complexed to a free protein fraction 1B [16], while the V and W antigens also optimally expressed at 37 7C and first described in Yersinia pestis have, in addition, been demonstrated in Yersinia enterocolitica and Yersinia pseudotuberculosis [16][17][18]. The somatic antigen of Yersinia pestis is rough (R-antigen) and is also present in Yersinia pseudotuberculosis [19].…”

Section: The Genus Yersiniamentioning

confidence: 99%

Yersiniosis I: Microbiological and Clinicoepidemiological Aspects of Plague and Non-Plague Yersinia Infections

Smego

Frean

Koornhof

1999

European Journal of Clinical Microbiology & Infectious Dise

120

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Yersinia

Bottone

Bercovier

Mollaret

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Yer.si' ni.a . M.L. fem. n. Yersinia named for the French bacteriologist A.J.E. Yersin, who first isolated the causal organism of plague in 1894. Proteobacteria / Gammaproteobacteria / Enterobacteriales / Enterobacteriaceae / Yersinia Straight rods to coccobacilli , 0.5–0.8 × 1–3 µm. Endospores are not formed. Capsules are not present, but an envelope occurs in Y. pestis strains grown at 37°C and in cells from clinical sources. Gram negative, nonmotile at 37°C, but motile with peritrichous flagella when grown below 30°C, except for Y. pestis , which is always nonmotile . Growth occurs on ordinary bacteriological media. Colonies of yersiniae are translucent to opaque, 0.1–1.0 mm in diameter after 24 h. Optimum growth temperature, 28–29°C. Facultatively anaerobic, having both a respiratory and a fermentative type of metabolism. Oxidase negative. Catalase positive. Nitrate is reduced to nitrite with a few exceptions in specific biovars. D ‐glucose and other carbohydrates are fermented with acid production but little or no gas. Phenotypic characteristics are often temperature dependent, and usually more characteristics are expressed by cultures incubated at 25–29°C than at 35–37°C . The enterobacterial common antigen is present in all species investigated. Widely distributed in nature with some species adapted to specific animal hosts and humans. Several species are pathogenic for humans and animals including Y. pestis , the causative agent of plague. A significant cause of food‐borne and waterborne disease. Belongs to the Gammaproteobacteria . The mol % G + C of the DNA is : 46–50. Type species : Yersinia pestis (Lehmann and Neumann 1896) Van Loghem 1944, 15 ( Bacterium pestis Lehmann and Neumann 1896, 194; Yersinia pseudotuberculosis subsp. pestis Bercovier, Mollaret, Alonso, Brault, Fanning, Steigerwalt and Brenner 1981a, 383.)

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Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis

Cited by 198 publications

References 12 publications

Cpx-signalling facilitates Hms-dependent biofilm formation by Yersinia pseudotuberculosis

Cpx-signalling facilitates Hms-dependent biofilm formation by Yersinia pseudotuberculosis

Yersiniosis I: Microbiological and Clinicoepidemiological Aspects of Plague and Non-Plague Yersinia Infections

Yersinia

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