Presence of Acyl-Homoserine Lactone in Subtidal Biofilm and the Implication in Larval Behavioral Response in the Polychaete Hydroides elegans

Huang, Yi L.; Dobretsov, Sergey; Ki, Jang Seu; Yang, Lai Hung; Qian, Pei‐Yuan

doi:10.1007/s00248-007-9210-9

Cited by 65 publications

(55 citation statements)

References 41 publications

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“…The observation that only the 1-and 3-day subtidal biofilms could induce coloration of the reporter strain CV026 was similar with that in our previous study, which was carried out at the university pier, a quite different costal marine environment (Huang et al, 2007a). These consistent results suggest that dynamics of AHLs in subtidal biofilms over time may follow similar pattern in coastal marine environments.…”

Section: Dynamics Of Ahls During Subtidal Biofilm Formationsupporting

confidence: 88%

“…AHL-based QS system was firstly discovered from marine Vibrio fischeri (Milton, 2006). Recently, AHL-producing bacteria were isolated from 'marine snow' (Gram et al, 2002), marine sponges (Taylor et al, 2004;Mohamed et al, 2008), marine water column, eukaryotic algae (Wagner-Dobler et al, 2005) and marine subtidal biofilms (Huang et al, 2007a), suggesting the potential impacts of AHLs in marine bacterial communities. It was proposed that bacteria might use QS as a weapon to compete for survival or to dominate in a natural niche (Waters and Bassler, 2005;Defoirdt et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Evidence for the dynamics of Acyl homoserine lactone and AHL-producing bacteria during subtidal biofilm formation

Huang

Lee

et al. 2008

The ISME Journal

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The quorum sensing signals-acyl homoserine lactones (AHLs) were directly detected in 1-9-dayold subtidal biofilms developed in a coastal fish farm by using AHL reporter strains and gas chromatography-mass spectrometry. Both methods showed that the AHL molecules and/or AHLproducing bacterial community were dynamic during biofilm development, with dominant AHLs changed from short-chain to long-chain AHLs. Terminal restriction fragment length polymorphism analysis of the bacterial 16S rRNA genes derived from subtidal biofilms of different ages was compared to that of the 21 AHL-producing bacteria isolated from the same batch of subtidal biofilms. All terminal restriction fragments (TRFs) generated from AHL-producing bacteria matched with the dominant TRFs derived from the biofilm bacterial community samples. Particularly, the TRFs of all AHL-producing Vibrio spp. matched with the TRFs that were dominant only in 1-day-old biofilm, suggesting that AHL-producing vibrios were one of the pioneer groups during subtidal biofilm formation. We reported here for the first time the dynamics of AHLs and AHL-producing bacteria during the formation of a subtidal biofilm.

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Section: Dynamics Of Ahls During Subtidal Biofilm Formationsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Evidence for the dynamics of Acyl homoserine lactone and AHL-producing bacteria during subtidal biofilm formation

Huang

Lee

et al. 2008

The ISME Journal

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“…(Joint et al 2002). In a previous study, we reported the direct detection of AHLs in marine subtidal biofilms and found that AHLs affected larval behavior in the polychaete Hydroides elegans (Huang et al 2007a). However, the diversity of AHLproducing bacteria in marine biofilms has not been shown.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 94%

Diversity and acyl-homoserine lactone production among subtidal biofilm-forming bacteria

Huang¹,

Ki²,

Case³

et al. 2008

Aquat. Microb. Ecol.

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Bacteria isolated from subtidal biofilms were identified via 16S rRNA gene sequencing and screened for acyl-homoserine lactone (AHL) production. In total, 68 strains were isolated from 1 to 9 d-old subtidal biofilms developed at a coastal fish farm. Identification based on partial 16S rRNA gene sequencing revealed that these isolates were distributed among 3 phylogenetic groups (4 Bacteroidetes, 13 α-Proteobacteria and 51 γ-Proteobacteria), with most isolates belonging to the family Rhodobacteraceae and the genera Thalassomonas, Alteromonas, Pseudoalteromonas, Shewanella and Vibrio. AHL screening was performed using 2 AHL reporter strains, Agrobacterium tumefaciens A136 and Chromobacterium violaceum CV026. Results showed that 21 strains (31%) produced AHLs, including 3 Bacteroidetes, 5 α-Proteobacteria and 13 γ-Proteobacteria. All the AHL-producing α-Proteobacteria belonged to the family Rhodobacteraceae, whereas the AHL-producing γ-Proteobacteria consisted of 6 Pseudoalteromonas spp., 6 Vibrio spp. and 1 Thalassomonas sp. This is the first report of AHL-producing marine bacteria in the genera Flammeovirga, Pseudoalteromonas and Thalassomonas. The family Rhodobacteraceae (11 isolates) and the genera Vibrio (15 isolates) and Pseudoalteromonas (17 isolates) had the greatest number of AHL-producing isolates. AHL profiling of the AHL-producing isolates was performed by GC-MS. Most AHL-producing isolates produced several different AHLs, many of which were long-chain-and 3-oxo-AHLs. The widespread occurrence of AHL-producing bacteria in subtidal biofilms suggests that AHLs may play a role in the community development in this environment. KEY WORDS: Subtidal biofilm · Marine bacteria · Acyl-homoserine lactone · AHL Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 52: [185][186][187][188][189][190][191][192][193] 2008 2005). Therefore, we assume that subtidal biofilms may harbor various kinds of AHL-producing strains.Molecular mechanisms of AHL-driven bacterial QS have been well studied in the lab; however, little work has been done concerning its ecological role in the field (McLean et al. 1997, Parsek & Greenberg 2005. It had been reported that the diffusible AHLs produced by bacterial biofilms increase spore settlement of the green alga Ulva sp. (Joint et al. 2002). In a previous study, we reported the direct detection of AHLs in marine subtidal biofilms and found that AHLs affected larval behavior in the polychaete Hydroides elegans (Huang et al. 2007a). However, the diversity of AHLproducing bacteria in marine biofilms has not been shown.In the present study, bacteria were isolated from 1 to 9 d old subtidal biofilms developed in a coastal fish farm of Hong Kong, identified by partial 16S rRNA gene sequencing, and screened for AHL production. AHLs produced by bacteria were identified by gas chromatography-mass spectrometry (GC-MS). MATERIALS AND METHODSDevelopment of subtidal biofilms. Subtidal biofilms were developed from 12 to 21 October 2006 at Yung Shu...

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“…Therefore, elimination of AHL may thus prevent the development of bacterial fouling [25]. AHL acylase degrades AHL, and as the concentration of this enzyme increases, biofilm formation is inhibited and the settlement of Ulva spores and polychaete larvae is affected [110,111]. Consequently, AHL acylases can also inhibit the settlement of macroorganisms to some extent.…”

Section: Enzymes That Interfere With Intercellular Communicationmentioning

confidence: 99%

Progress of marine biofouling and antifouling technologies

et al. 2010

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Presence of Acyl-Homoserine Lactone in Subtidal Biofilm and the Implication in Larval Behavioral Response in the Polychaete Hydroides elegans

Cited by 65 publications

References 41 publications

Evidence for the dynamics of Acyl homoserine lactone and AHL-producing bacteria during subtidal biofilm formation

Evidence for the dynamics of Acyl homoserine lactone and AHL-producing bacteria during subtidal biofilm formation

Diversity and acyl-homoserine lactone production among subtidal biofilm-forming bacteria

Progress of marine biofouling and antifouling technologies

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